Abdelaziz Elrifaie

Abdelaziz Elrifaie

Beni-Suef University

H-index: 8

Africa-Egypt

Abdelaziz Elrifaie Information

University

Beni-Suef University

Position

Professor and Chairman of Dermatology

Citations(all)

169

Citations(since 2020)

107

Cited By

106

hIndex(all)

8

hIndex(since 2020)

6

i10Index(all)

8

i10Index(since 2020)

5

Email

University Profile Page

Beni-Suef University

Abdelaziz Elrifaie Skills & Research Interests

Chairman of Dermatology Department

Top articles of Abdelaziz Elrifaie

Assessment of cbiL gene expression and vitamin B12 levels in acne vulgaris: involvement of both in-vivo and in-vitro studies

Acne vulgaris (AV) is a chronic inflammatory disease of the pilosebaceous follicles that affects nearly 90% of teenagers; half of them continue to experience symptoms as adults. There have been several reports of monomorphic acneiform eruptions in patients treated with intramuscular cobalamin injections. The eruptions resolved after the cessation of the therapy. Cyanocobalamin, pyridoxine (B6), and riboflavin (B2) have been reported to exacerbate existing acne. Moreover, it was reported that in acne patients, the serum level of vitamin B12 was significantly decreased after treatment, but no explanation was given. We propose a vitamin B12-mediated bacterial mechanism for acne pathogenesis on the evidence mentioned above. One hundred twenty AV patients were recruited.     Our results proved that vitamin B12 was involved in the pathogenesis of AV; in vivo, it proved that AV patients had significantly higher serum levels of the vitamin than controls and showed a positive correlation to the disease duration and severity. In vitro, it proved that vitamin B12 supplementation to p. acnes cultures significantly raised the porphyrins produced in these cultures when compared to the non-supplemented ones. It also proved that this shift in the metabolism is secondary to inhibition of the cbiL gene of the vitamin B12 synthesis in the bacteria; as porphyrins and vitamin B12 share the same precursors, vitamin B12 does has a role in either elicitation or aggravation of AV.

Authors

Aly Atef Shoun,Ahmed Abd El-Naser Mohamed,Gina Salah Fouad,Abd-El Aziz El Rifaie,Naglaa Abd-El Moniem Radi

Journal

Journal of Bioscience and Applied Research

Published Date

2023/6/1

SOCS3 expression in cutaneous lichen planus

Background: Lichen Planus (LP) is an idiopathic, inflammatory, autoimmune disease. The aetiopathogenesis of LP is still unclear. Suppressor of cytokine signaling-3 (SOCS3) is known to inhibit cytokine signaling through Janus kinase (JAK)/signal transducers, activators of transcription, NFkB, and focal adhesion kinase (FAK) signaling pathways. Modulation of SOCS3 level leads to immune dysfunction.Aim of the work: Our study aimed to detect a possible role of SOCS3 in cutaneous LPMethods: In this case-control study skin biopsies were collected from 30 LP patients and 30 healthy volunteers. Biopsies were kept in lysis solution frozen at-80 Celsius till analysis of SOCS3 using Real time polymerase chain technique (RT-PCR) was done.Results: The expression of SOCS3 in cutaneous LP was significantly higher when compared to normal skin from healthy controls (p< 0.001).Conclusion: SOCS3 might play a role in the pathogenesis of LPSuppressor of cytokine signaling-3SOCS3Lichen planusqRT PCRFiguresFigure 1Figure 1IntroductionThe suppressors of cytokine signaling (SOCS) are inhibitors of cytokine signaling that function via the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathways. 1

Authors

Reham William Doss,Abdel-Aziz El-Rifaie,Laila Ahmed Rashed,Rehab Naguib

Published Date

2023/4/10

An insight into the value of circular RNA in psoriasis pathogenesis and its correlation with PASI score

BackgroundPsoriasis is a chronic hyperproliferative inflammatory disease, in which genetic and environmental factors have an important role, but the exact cause is yet unknown. Circular RNAs (circRNAs) are regulatory noncoding RNAs. They play an important role in multiple physiological and pathological processes, such as cancer, cardiovascular diseases, and neuronal diseases. Some circRNAs are involved in regulating immunity, inflammation, and cell proliferation in many types of cells.AimTo detect the expression of circRNA (Circ0061012) in psoriatic skin of diseased patients as compared with normal control persons to investigate the possible role of circRNA in psoriasis pathogenesis and its correlation with disease severity.

Authors

Rehab M Naguib,Abd-El A El-Rifaie,Abd-El A El-Taweel,Mohamed MFAA Dahab

Journal

Egyptian Journal of Dermatology and Venerology

Published Date

2022/9/1

Usefulness of Vaspin Marker and C-reactive Protein in Assessment of the Severity of Psoriasis

This study aims to study the level of Vaspin and CRP in the serum of psoriatic patients as compared to normal control persons and to correlate their levels with the severity of the disease. Methods 30 psoriasis patients as cases vs 30 normal patients as control were tested for CRP and Vaspin correlating the results with the Psoriasis Severity Index PSI. Results There was a highly statistically significant difference between cases and controls regarding their VASPIN and CRP levels (P-value<0.001). in cases there was a significant positive moderate linear correlation between CRP and PASI. There was a significant strong negative linear correlation between the VASPIN and the PASI score (P-value=0.009), showed that at a cut off 75.6 of vaspin predicted that the patient had no psoriasis with 100% sensitivity and 75.6% specificity. Conclusions CRP and Vaspin could be used as markers to assess the severity of the disease and treatment response.

Authors

Aya Emad Eldin Mohamed Sadek,Abd El-Aziz El Rifaie,Laila Ahmed Rashed,Rehab Mohamed Naguib

Journal

Egyptian Journal of Medical Research

Published Date

2022/1/1

Detection of Desmoglein-3 Auto-Antibodies in Patients with Lichen Planus

There is controversy about the role of auto anti-bodies against desmoglein 3 (Dsg 3) in the pathogenesis of lichen planus (LP). The goal of this study is to detect desmoglein-3 auto-antibodies in lichen planus patients comparted to normal control persons via investigating its potential role in the pathogenesis of this disease. A case control study for detection of Desmoglein-3 autoantibodies in serum of 40 lichen planus patients and 40 healthy controls using enzyme-linked immunosorbent assay (ELISA) technique.The patients and healthy controls were recruited from Dermatology outpatient clinic at Beni-Suef University hospital. The measurement of anti Dsg3 antibodies in LP patients was significantly higher as compared to normal control persons. The mean scores were 1361.37 ± 300.5 in oral LP & 2118.70 ± 584.37 in cutaneous LP vs. 66.98 ±70.95 in controls (p-value < 0.001). Desmoglein-3 auto-antibodies in LP may have a role in the pathogenesis of the disease. Further investigations are needed for detection of Dsg 3 auto-antibodies in LP skin biopsy or blood samples by other methods such as Immunofluorescence.

Authors

Abd-El Aziz El-Rifaie,Laila Ahmed Rashed,Mona El Sayed Ahmed Abdellatif,Asmaa Abdel Ghany Mohamed

Journal

Egyptian Journal of Medical Research

Published Date

2022/7/1

Study Of Role Of Lipoxin–A4 In Psoriasis

The aim of this work was to evaluate the level of lipoxin – A4 (LXA4) in patients  with psoriasis and its correlation with clinical parameters. This study included 25 psoriatic patients who were recruited from the outpatient clinics of dermatology department, Faculty of Medicine, Beni-Suef University. In addition, 25 age and sex-matched volunteers were included as control group. Four mm punch skin biopsy will be taken from the tissue and will be kept in lysis solution for the stability of the studied parameters and will be kept frozen at -80°C till detection of LXA4 in tissue by real time-PCR. Peripheral blood samples for serum LXA4 are taken. Blood samples will be centrifuged and serum will be stored at 80°C and used for ELISA. The LPXA levels in the case ranged from 1.01 -7.8 ng/mL with a mean of 2.94 ±1.7 ng/mL. In addition, the LXA4 levels in the control ranged from 4.5 – 14.7 ng/mL with a mean of 9.45 ±2.26 ng/mL. The level of LXA4 is significantly downregulated in patients with psoriasis, compared to healthy volunteers. Moreover, the affection of LXA4 appears to be correlated with the severity of the diseases. LXA4 and its analogue may be potential therapeutic candidates for psoriasis.

Authors

Al Nemr,Hanan Abo Zaid Mohamed,Abd El Aziz El-Rifaie,Rehab Mohamed Nagib,Laila Ahmed Rashed

Journal

Egyptian Journal of Medical Research

Published Date

2022/1/1

Estimation of Advanced Glycation end-Products in Patients of Vitiligo

The goal of this study is to detect a relationship between advanced glycation end-products and the pathogenesis of vitiligo in diseased patients as compared to normal control persons using Enzyme-Linked Immunosorbent Assay (ELISA) technique on tissue skin biopsy. The present study included 30 Egyptian vitiligo patients, their age ranged from (20 to 50 years). They were recruited from Dermatology outpatient clinic at Beni-Suef University hospital. Thirty unrelated apparent healthy controls with similar demographic (matched age and sex) were taken.The studied subjects were divided into two groups as follows, Group I: (n = 30) vitiligo patients and Group II: (n = 30) unrelated apparent healthy controls. Four mm punch skin biopsy will be taken from patients( vitiligo lesion) & control and it will be kept in lysis solution for the stability of the studied parameters and will be kept frozen at -80 Celsius till analysis of  advanced glycation end-products by ELISA. The result of the study showed that expression of AGEs in lesional skin of Vitiligo patients was significantly higher as compared to normal control persons.  Also reveled moderate negative correlation between the expression of Advanced Glycation end-Products (AGEs) in lesional skin of vitiligo patients and Vitiligo disease activity (VIDA) score of the studied cases and that confirms that AGE levels were, instead, significantly higher in vitiligo patients with older lesions.

Authors

Amany Gamal Ali,Mona El Sayed Ahmed,Abd-El Aziz El-Rifaie

Journal

Egyptian Journal of Medical Research

Published Date

2022/1/1

Tissue Expression of Visfatin in Psoriatic Patients

Background Psoriasis is a chronic disease, in which genetic, immunological, and environmental factors play a significant role. The immunological mechanism involved in pathogenesis causes cutaneous inflammation and keratinocytes hyperproliferation. Subcutaeous adipose tissue secretes adipokines which play a role in cutaneous inflammation. Visfatin is an adipokine that induces inflammatory process by increasing cytokines production and activation. It affects inflammatory and immune response through induction of chemotaxis. Aim of this work To estimate visfatin level in lesional tissues of psoriatic patients in comparison with healthy controls and its correlation with disease severity. Patients and Methods The expression of visfatin was detected in the skin of 30 psoriasis patients and 30 healthy controls using using ELISA technique. Results Visfatin expression was significantly higher in psoriasis patients compared to healthy controls. In addition, there was no significant difference between visfatin expression and severity of the disease.  Conclusion Visfatin may play a role in pathogenesis of psoriasis.

Authors

Abd El-Aziz El-Rifaie,Laila Ahmed Rashed,Ahlam Hosny Abd Alla,Doaa Gamal Zaki

Journal

Egyptian Journal of Medical Research

Published Date

2022/10/1

Downregulation of microRNA-520 in chronic plaque psoriasis

BackgroundPsoriasis is a chronic disease, in which genetic and environmental factors play an important role, but the exact cause is yet unknown. It includes inflammation and increased proliferation of skin cells. MicroRNAs (miRNAs) are small, single-stranded,∼ 22-nucleotide-long, noncoding gene-regulatory RNA molecules that inhibit gene expression through translational repression or mRNA turnover. MicroRNA-520 suppresses the mitotic entry and proliferation of keratinocytes.AimThis case–control study is to detect the expression of miRNA-520 in serum samples of diseased patients as compared with normal control persons to investigate the possible role of miRNA-520 in the pathogenesis of psoriasis.

Authors

Rehab M Naguib,Abd-El A El-Rifaie,Manar A Awad,Laila A Rashed

Journal

Egyptian Journal of Dermatology and Venerology

Published Date

2022/1/1

The importance of micro-RNA 224-3p in pathogenesis of NonSegmental Vitiligo

BackgroundVitiligo is a skin and less common hair disease characterized by a decline in melanocyte function with a prevalence of 0.5–1% in most populations. Micro-RNAs (miRNAs) are single-stranded, noncoding, short RNA molecules, they act as regulators of gene expression and play critical roles in many biological processes such as differentiation, development, and metabolism of the human-body cells.AimThis case–control study aimed at detection of the expression of miRNA-224-3p in lesional skin of vitiligo patients as compared with normal-control persons to detect the possible role of miRNA-224-3p in the pathogenesis of vitiligo.

Authors

Rehab M Naguib,Hanan A Ibrahim,Laila A Rashed,Abd-El A El-Rifaie

Journal

Egyptian Journal of Dermatology and Venerology

Published Date

2022/9/1

Study of detection of serum CXCL10 in vitiligo patients

The goal of this study was to detect the level of CXCL10 in the serum of  the vitiligo patients as compared to the normal control persons to investigate the possible role of CXCL10 in the pathogenesis of  this disease. The present study included 30 Egyptian vitiligo patients, their age ranged from (20 to 50 years). They were recruited fromDermatology outpatient clinic at Beni-Suef University hospital. Thirty unrelated apparent healthy controls with similar demographic (matched age and sex) were taken during the period from (1st May 2018 to 1st May 2019).5ml blood sample was taken from patients and controls and  was left to clot for 30 minutes then serum was separated  by centrifugation and kept frozen at -80c till analysis of CXCL10 by ELISA. The patients and controls were subjected to estimation of the serum level of CXCL10. The result of the study showed that  increased levels of CXCL10 in the vitiligo patients when compared to controls and the  patients in the progressive stage showed higher expression of CXCL10 than those in the stable stage,  suggesting a mechanistic  role of CXCL10 in vitiligo.

Authors

Abd-El Aziz El-Rifaie,Rehab Mohamed Naguib,Laila Ahmed Rashed,Marwa Fathelbab Ahmed

Journal

Egyptian Journal of Medical Research

Published Date

2022/1/1

The role of autophagy-related gene 9B in lichen planus

Background Lichen planus (LP) is an idiopathic, chronic, relapsing, inflammatory, autoimmune dermatological disease. The etiopathogenesis of LP is still unclear. Autophagy is a strictly regulated lysosomal degradation pathway that is crucial for maintaining intracellular homeostasis and normal development. The dysregulation of autophagy-associated genes was recognized to increase the susceptibility to multiple diseases, including inflammation, autoimmune disorders and cancer. Aims Our study aimed to detect the expression of autophagy-related gene 9 b (ATG9B) in LP patients compared to normal control persons to investigate the possible role of autophagy in pathogenesis of this disease. Methods This case–control study included 30 LP patients and 30 age-, gender-matched healthy controls. Four millimeters punch skin biopsies were obtained from LP lesions and from the controls and they were kept in lysis solution for the stability of the studied parameters and were kept frozen at –80°C till analysis of ATG9B using real-time polymerase chain reaction. Results The level of ATG9B in lesional skin of LP was significantly decreased compared to normal control persons (P < 0.01); also, there was a non-significant relation between ATG9B level and age, sex, duration and family history among LP patients. Limitations Limited number of patients included in our study (30 patients). Conclusion Autophagy may play a role in the pathogenesis of cutaneous LP.

Authors

Rehab Mohamed Naguib,Ayat Mohammed Abd El Wahab,Laila Ahmed Rashed

Journal

Indian Journal of Dermatology, Venereology and Leprology

Published Date

2021/12/31

Expression of MicroRNA-224-3p in vitiligo.

Background Vitiligo is a skin and less common hair disease characterized by decline melanocytes function and depigmentation with a prevalence of 0.5-1% in most populations. MicroRNAs (miRNAs) are small single stranded; approximately 22 nucleotide-long, non-coding generegulatory RNA molecules that inhibit gene expression through translational repression or mRNA turnover. Objective This case control study aimed at detection of the expression of microRNA-224-3p in lesional skin of vitiligo patients as compared to normal control persons to detect the possible role of microRNA-224-3p in the pathogenesis of vitiligo. Methods This study included 60 patients with vitiligo and 60 age, gender matched healthy controls. 4 mm punch skin biopsy had been taken from patients (vitiligo lesion) & controls and it had been kept in lysis solution for the stability of the studied parameters and was kept frozen at-80 Celsius till …

Authors

Abd-El Aziz El-Rifaie,Rehab Mohamed Naguib,Hanan Ahmed Ibrahim,Laila Ahmed Rashed

Journal

Journal of Pakistan Association of Dermatologists

Published Date

2021/10/1

COMPARISON BETWEEN THE EFFECT OF TITANIUM DIOXIDE NANOPARTICLES AND IVERMECTIN ON HEAD LICE

Head lice cause social problems for patients as embarrassment, inflammation, skin irritationand secondary bacterial infection. Treating head lice is difficult because of development of resistanceto drug. In this study, our aim was to evaluate the effect of titanium dioxide, Ivermectinand Ivermectin-loaded on Titanium dioxide nanoparticles on head lice in vitro. This study wasconducted on 50 patients infected with head lice from Beni Suef Governorate. These patientswere of all age groups and both sexes.Patients were 13 males and 37 females, with ages ranged from 5 to72years (26.84±19.3). Theywere 34 rural residents, and 16 urban ones, 37 were at a low socioeconomic level and 13 at amoderate socioeconomic one. Number of family members ranged from (3 to 11) with an averageof (6.42±1.9).The results showed that mortality rate for topical Ivermectin (0.2% & 0.5%) was 86% & 94%,respectively, against adult head lice, while 88% and 94%, respectively, against nymphs.Regarding the effect of Titanium dioxide, the percentage mortality effects of Titanium dioxidewere 34, 58, & 100% at (2, 6, & 10 mg/L), respectively, against adult head lice, while Titaniumdioxide mortality rate was 40, 68 & 96% at (2, 6 &10 mg/L), respectively, against nymphs.

Authors

SAMAH S ABDEL GAWAD,ABDEL-AZIZ EL-RIFAIE,HANAN ABD ELRAZIK KAMEL,SHIMAA S IBRAHIM,MONA IBRAHIM ALI

Journal

Journal of the Egyptian Society of Parasitology

Published Date

2021/8/1

Estimation of the Level of Interlukien-33 and Interferon–γ in Lesional and Nonlesional Tissues of Patients with Viral Warts and in Healthy Controls

Background Viral warts are benign skin growths that are caused by Human Papilloma Virus (HPV). There are many types of warts either genital or non-genital. Interlukien-33 (IL-33) is a member of IL-1 family. It acts as alarmin molecule that provides protective antiviral immunity and plays an important role in many diseases. Interferon- γ (IFN-γ) has a well-known antiviral role and improves the expression of IL-33 in human keratinocytes in various diseases. Aim of this work Estimation of the level of IL-33 and IFN-γ in lesional and non-lesional tissues of patients with viral warts and in healthy controls and evaluate their role in the pathogenesis of viral warts. Subjects and Methods We did our study on two groups; 25 patients with viral warts and 25 matching relatively healthy controls. We used enzyme linked immunosorbent assay (ELISA) kits to measure the levels of IL-33 and IFN-γ in 4mm diameter skin biopsies from the lesion itself, the non-lesional skin of the same patients and from skin of healthy controls to compare between the results. Results The levels of IL-33 and IFN-γ in lesional and non-lesional tissues of viral warts were significantly higher than in healthy controls. Conclusion Our presenting study is suggesting that IL-33 and IFN-γ may play a role in the pathogenesis of viral warts.

Authors

Abd El-Aziz El-Rifaie,Laila A Rashed,Ahlam H AbdAllah,Maha I Taghyan

Journal

Minia Journal of Medical Research

Published Date

2020/4/1

Cutaneous syndecan-1 expression before and after phototherapy in psoriasis

Syndecans are transmembrane proteins that comprise a major family of cell surface heparan sulfate proteoglycans. Syndecan‑1, present on the surface of keratinocytes, has the ability to interact with other cell surface receptors and to regulate their actions, controlling cell adhesion, migration and proliferation. 1The expression of syndecan is markedly diminished in diseases that exhibit acantholysis, spongiosis or loss of cellular adhesion such as pemphigus vulgaris, bullous pemphigoid and herpes simplex. 1 Its serum levels has also been used as a marker of severity of inflammation in Crohns disease. 2

Authors

Reham William Doss,Abdel-Aziz El-Rifaie,Aya Nagy Said,Laila A Rashed

Journal

Indian Journal of Dermatology, Venereology and Leprology

Published Date

2020/7/1

See List of Professors in Abdelaziz Elrifaie University(Beni-Suef University)

Abdelaziz Elrifaie FAQs

What is Abdelaziz Elrifaie's h-index at Beni-Suef University?

The h-index of Abdelaziz Elrifaie has been 6 since 2020 and 8 in total.

What are Abdelaziz Elrifaie's top articles?

The articles with the titles of

Assessment of cbiL gene expression and vitamin B12 levels in acne vulgaris: involvement of both in-vivo and in-vitro studies

SOCS3 expression in cutaneous lichen planus

An insight into the value of circular RNA in psoriasis pathogenesis and its correlation with PASI score

Usefulness of Vaspin Marker and C-reactive Protein in Assessment of the Severity of Psoriasis

Detection of Desmoglein-3 Auto-Antibodies in Patients with Lichen Planus

Study Of Role Of Lipoxin–A4 In Psoriasis

Estimation of Advanced Glycation end-Products in Patients of Vitiligo

Tissue Expression of Visfatin in Psoriatic Patients

...

are the top articles of Abdelaziz Elrifaie at Beni-Suef University.

What are Abdelaziz Elrifaie's research interests?

The research interests of Abdelaziz Elrifaie are: Chairman of Dermatology Department

What is Abdelaziz Elrifaie's total number of citations?

Abdelaziz Elrifaie has 169 citations in total.

What are the co-authors of Abdelaziz Elrifaie?

The co-authors of Abdelaziz Elrifaie are Reham William Naguib Doss.

    Co-Authors

    H-index: 6
    Reham William Naguib Doss

    Reham William Naguib Doss

    Beni-Suef University

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