Abdel Nasser B.Singab

Abdel Nasser B.Singab

Ain Shams University

H-index: 41

Africa-Egypt

About Abdel Nasser B.Singab

Abdel Nasser B.Singab, With an exceptional h-index of 41 and a recent h-index of 36 (since 2020), a distinguished researcher at Ain Shams University, specializes in the field of Phytochemistry, hepatoprotective effects of natural products, Pharmacognosy, Phytotherapy.

His recent articles reflect a diverse array of research interests and contributions to the field:

The impact of seasonal variation on the composition of the volatile oil of Polyalthia suberosa (Roxb.) Thwaites leaves and evaluation of its acetylcholinesterase …

Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities

Dehydrocostus lactone from the root of Ajuga integrifolia (Buch.-Ham. Ex D. Don): Quantitative determination and in-silico study for anti-breast cancer activity

Unveiling the Antimicrobial and Larvicidal Potential of Butyrolactones and Orsellinic Acid Derivatives from the Morus alba‐derived Fungus Aspergillus terreus via Integrated In …

Benzohydrazide as a good precursor for the synthesis of novel bioactive and anti-oxidant 2-phenyl-1, 3, 4-oxadiazol-aminoacid derivatives: Structural determination, biological …

Medicinal and aromatic plants-I

Role of Nutraceuticals in Obesity Management: A Mechanism and Prospective Supported by Molecular Docking Studies

Unearthing the Potential Secondary Metabolites Isolated from the Fungus Aspergillus flocculosus: A Comprehensive Review.

Abdel Nasser B.Singab Information

University

Ain Shams University

Position

Vice President for Postgraduate and Research Egyptn

Citations(all)

5740

Citations(since 2020)

3855

Cited By

3116

hIndex(all)

41

hIndex(since 2020)

36

i10Index(all)

138

i10Index(since 2020)

112

Email

University Profile Page

Ain Shams University

Abdel Nasser B.Singab Skills & Research Interests

Phytochemistry

hepatoprotective effects of natural products

Pharmacognosy

Phytotherapy

Top articles of Abdel Nasser B.Singab

The impact of seasonal variation on the composition of the volatile oil of Polyalthia suberosa (Roxb.) Thwaites leaves and evaluation of its acetylcholinesterase …

Authors

Orchid A Mahmoud,Iriny M Ayoub,Omayma A Eldahshan,Abdel Nasser B Singab

Journal

BMC Complementary Medicine and Therapies

Published Date

2024/4/12

BackgroundPolyalthia suberosa (Roxb.) Thwaites (Annonaceae) is a medicinal plant that has been reported for its various pharmacological potentials, such as its anti-inflammatory, analgesic, antioxidant, and neuropharmacological activities. This study aimed to analyze the leaf essential oils of P. suberosa (PSLO) collected in different seasons, to evaluate the acetylcholinesterase inhibitory activity, and to corroborate the obtained results via in-silico molecular docking studies.MethodsThe leaf essential oils of P. suberosa collected in different seasons were analyzed separately by GC/MS. The acetylcholinesterase inhibitory activity of the leaves oil was assessed via colorimetric assay. In-silico molecular docking studies were elucidated by virtual docking of the main compounds identified in P. suberosa leaf essential oil to the active sites in human acetylcholinesterase crystal structure.ResultsA total of 125 compounds …

Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities

Authors

Shaza H Aly,Ahmed M Elissawy,Mahmoud A El Hassab,Taghreed A Majrashi,Fatma E Hassan,Eslam B Elkaeed,Wagdy M Eldehna,Abdel Nasser B Singab

Journal

Journal of Enzyme Inhibition and Medicinal Chemistry

Published Date

2024/12/31

This study aims to investigate the phytoconstituents of the chloroform fraction of three Cystoseira spp. namely C. myrica, C. trinodis, and C. tamariscifolia using UPLC/ESI/MS technique. The results revealed the identification of 19, 20 and 11 metabolites in C. myrica, C. trinodis, and C. tamariscifolia, respectively mainly terpenoids, flavonoids, phenolic acids and fatty acids. Also, an in vitro antioxidant study using FRAP and DPPH assays was conducted where the chloroform fraction of C. trinodis displayed the highest antioxidant activity in both assays, which would be attributed to its highest total phenolics and total flavonoids. Besides, the investigation of COX-1, α-glucosidase and α-amylase inhibitory activities were performed. Regarding C. trinodis, it showed the strongest inhibitory activity towards COX-1. Moreover, it showed potent inhibitory activity towards α-glucosidase and α-amylase enzymes. According to the …

Dehydrocostus lactone from the root of Ajuga integrifolia (Buch.-Ham. Ex D. Don): Quantitative determination and in-silico study for anti-breast cancer activity

Authors

Fekade Beshah Tessema,Yilma Hunde Gonfa,Tilahun Belayneh Asfaw,Mesfin Getachew Tadesse,Archana Joshi Bachheti,Abdel Nasser Singab,Rakesh Kumar Bachheti

Journal

Plant Science Today

Published Date

2024/1/1

Many biological activities were reported for the Ajuga species, specifically for Ajuga integrifolia and its synonyms. These include antioxidant, anti-inflammatory, antidiabetic, antibacterial, blood purifier effects, and anticancer activity. This study quantitatively determines dehydrocostus lactone (DHCL) from the root of Ajuga integrifolia and its in silico study for anti-breast cancer activity. Camag HPTLC was used for TLC–densitometric estimation of dehydrocostus lactone. Estrogen receptor alpha (ER?) protein (PDB ID: 3ERT) was selected for its involvement in cell proliferation within the breast cancer cell. Tamoxifen is a reference drug commonly used in hormonal therapy, and DHCL was used as a ligand. Molecular docking was performed using AutoDock Vina in PyRx v. 0.8 to get the best

Unveiling the Antimicrobial and Larvicidal Potential of Butyrolactones and Orsellinic Acid Derivatives from the Morus alba‐derived Fungus Aspergillus terreus via Integrated In …

Authors

Khadiga Amr,Ahmed M Elissawy,Nehal Ibrahim,Mohamed S Elnaggar,Iten M Fawzy,Abdel Nasser B Singab

Journal

Chemistry & Biodiversity

Published Date

2024/3

The emergence of multi‐drug‐resistant microbial strains spurred the search for antimicrobial agents; as a result, two distinct approaches were combined: four in vitro studies and four corresponding molecular docking investigations. Antituberculosis, anti‐methicillin‐resistant Staphylococcus aureus (anti‐MRSA), antifungal, and larvicidal activities of the crude extract, two fractions, and seven isolated compounds from Aspergillus terreus derived from Morus alba roots were explored. The isolated compounds (5 butyrolactones and 2 orsellinic acid derivatives) showed potent to moderate antitubercular activity with MIC values ranging from 1.95 to 62.5 μg/mL (compared to isoniazid, 0.24 μg/mL) and promising anti‐MRSA potential with inhibition zone diameters ranging from 8 to 25 mm. Additionally, the in silico study proved that the isolated compounds bind to the two corresponding proteins’ active sites with high to …

Benzohydrazide as a good precursor for the synthesis of novel bioactive and anti-oxidant 2-phenyl-1, 3, 4-oxadiazol-aminoacid derivatives: Structural determination, biological …

Authors

Khaled Briki,Talal Lahreche,Mouna Souad Abbassi,Mokhtar Boualem Lahrech,Adil Ali Othman,Ahmed M Elissawy,Abdel Nasser B Singab

Journal

Arabian Journal of Chemistry

Published Date

2024/6/1

The synthesis and biological assessment of 2,5-disubstituted-1,3,4-oxadiazole derivatives from benzo hydrazide and amino acids as novel potential antioxidant and antibacterial agents have been reported. The structures of the new compounds were characterized by physicochemical properties and spectral methods. The synthesized compounds were screened for their in vitro antibacterial activity against three Gram-positive bacterial strains, namely Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 14579, Listeria innocua ATCC 33090, and two Gram-negative bacterial strains, namely Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and antifungal activity against Candida albicans ATCC 10231 in comparison with Amoxicillin, Tetracycline, Gentamicin and Oxacillin as standards. Most of the compounds have excellent efficacy, and some of them, such as 5i, 5g, 5d, 5c, and 5j can …

Medicinal and aromatic plants-I

Authors

Soumya Sinha,Sana Tabanda Saeed,Asifa Khan,Abdul Samad

Published Date

2024/1/1

Plant viruses have obvious importance for food crops and ornamental plants, and a range of viruses are responsible for an estimated $60 billion in crop losses worldwide each year. Medicinal and aromatic plants (MAPs), viz., Andrographis paniculata, Catharanthus roseous, Mentha spp., Hyoscyamus spp., and Senna spp. become very important globally as their demands increase in pharmaceutical, nutraceuticals, immunological, fragrance, and flavor industries. These MAPs have been found to be infected by different types of plant viruses such as Potyvirus, Begomovirus, Tobamovirus, Tobravirus, Cucumovirus, Closterovirus, Tospovirus, and Potexvirus.

Role of Nutraceuticals in Obesity Management: A Mechanism and Prospective Supported by Molecular Docking Studies

Authors

Abdel Nasser B Singab,Esraa A Elhawary,Yasmin A Elkhawas,Iten M Fawzy,Ashaimaa Y Moussa,Nada M Mostafa

Journal

Journal of Medicinal Food

Published Date

2024/2/6

Obesity and its comorbidities represent a major health problem worldwide. Treatment by reducing food intake and physical activity interventions has limited success especially with elderly people with chronic diseases. Nutraceuticals are naturally originated and successfully used for their physiological and nutritional benefit in health care. They might be alternative means to help lose weight and reduce obesity-associated metabolic disorders with the improvement of health, delay the aging process, prevention of chronic diseases, increase of life expectancy, or support to the structure or function of the body. The current study enumerates the inherent role of nutraceuticals in the management of obesity and its related comorbidities. The study is supported with the molecular docking studies discussing the mechanism of action. An attempt to optimize the role of nutraceuticals is made in this article in addition to widen the …

Unearthing the Potential Secondary Metabolites Isolated from the Fungus Aspergillus flocculosus: A Comprehensive Review.

Authors

Ashraqat Ismail,Ahmed M Elissawy,Haidy A Gad,Abdel-Nasser Singab

Published Date

2024/6/1

Aspergillus flocculosus represents a relatively recent fungal source of interesting secondary metabolites. A. flocculosus, as a fungal endophyte, had been isolated from different sources including plants such as Markhamia platycaly, sponges, for example, Phakellia fusca, algae for example Padina sp. as well as sea sediment. This review presents a literature survey for the reported secondary metabolites isolated from A. flocculosus, demonstrating the potential biological activities of the reported metabolites. This review presents a comprehensive literature survey of the secondary metabolites isolated from the fungus A. flocculosus reporting eighty secondary metabolites belonging to different chemical classes including steroids, meroterpenoids and diketopiperazine alkaloids, drimane sesquiterpenoids as well as their nitro benzoyl derivatives as well as cerebrosides. Secondary metabolites reported from A. flocculosus possess numerous biological activities including anticancer, anti-inflammatory, antitrypanosomal, antimicrobial, and neuroprotective activities. Despite the relatively limited reports for secondary metabolites represented in this review, the chemical diversity and the potential biological activities of the reported metabolites reported herein encourage further in-depth research for novel bioactive secondary metabolites from A. flocculosus especially those derived as endophytes from marine resources.

Genus Curcuma: chemical and ethnopharmacological role in aging process

Authors

Esraa A Elhawary,Ashaimaa Y Moussa,Abdel Nasser B Singab

Published Date

2024/1/11

Aging or senescence is part of human life development with many effects on the physical, mental, and physiological aspects which may lead to age-related deterioration in many organs. Genus Curcuma family Zingieraceae represents one of the well-studied and medically important genera with more than eighty species. The genus is reported to contain different classes of biologically active compounds that are mainly presented in diphenylheptanoids, diphenylpentanoids, diphenylalkanoids, phenylpropene derivatives, alkaloids, flavonoids, chromones, terpenoids, phenolic acids and volatile constituents. Rhizomes and roots of such species are rich with main phytoconstituents viz. curcumin, demethoxycurcumin and bis-demethoxycurcumin. A wide variety of biological activities were demonstrated for different extracts and essential oils of genus Curcuma members including antioxidant, anti-inflammatory, cytotoxic …

Synthesis, characterization, antimicrobial and antioxidant activity of 2-(2′-hydroxyphenyl)-1, 3, 4-oxadiazolyl-5-amino acid derivatives

Authors

Sultan T. Abu-Orabi Asma K. Alshamari,Mahmoud A. Al-Qudah,Fedaa Hamadeh,Lo’ay A. Al-Momani

Journal

Letters in Organic Chemistry

Published Date

2022

For the reason of distinct place in the field of medicinal and pharmaceutical chemistry of 1,2,4-triazole derivatives, a new class of fused 1,2,4-triazolyl-isoxazole moieties was prepared from 3- (2,4,6-trimethoxyphenyl)isoxazolo-4,5-bis[carbonyl-(4#141;-phenyl) thiosemicarbazide via dehydration reactions of carbohydrazides by using the appropriate chemical reagents. The structures of the compounds were elucidated by both elemental and spectral (IR, NMR and MS) analyses. The in vitro antioxidant activity of the new compounds was determined by free radical scavenging and metal chelating activity. All the synthesized compounds showed good activity according to free radical scavenging and metal chelating activity compared with standards. The new compounds were screened in vitro antibacterial activity against three gram-positive bacteria and three gram-negative bacteria.

Egyptian plants stand a shield against diabetic mellitus: Scientific evidence and mechanistic approaches

Authors

Dina M Bahgat,Shaza H Aly,Heba AS El-Nashar,Ahmed E Elissawy,Omayma A Eldahshan,Abdel Nasser B Singab

Published Date

2024/1/1

Diabetes mellitus (DM) is considered an endocrine disease leading to a disorder in glucose metabolism and caused by deficiency of insulin release (type 1) or insulin insensitivity (type 2), affecting about 10% of the world’s population. Egypt occupies the ninth position in the prevalence of DM. Conventional hypoglycemic drugs such as metformin, acarbose voglibose, glimepiride, and pioglitazone are well known for their side effects like gastrointestinal disorders and vitamin B12 deficiency. Thus, there is continuous research for the development of an effective antidiabetic drug with fewer side effects since the treatment is long term for the rest of life. Since ancient times, daily Egyptian food has been rich in functional antidiabetic foods such as Raphanus sativus (Radish), Lupinus albus (termes), and Trigonella foenum-graecum (fenugreek). A wide array of Egyptian plants stands as a wealthy source of antidiabetic …

Acremochlorin O and Other Prenylated Chlorophenol Antimicrobial Metabolites from the Fungus Acremonium sp. Strain MNA-F-1

Authors

Mohamed S Elnaggar,Nada M Mostafa,Ahmed M Elissawy,Kunthida Phutthacharoen,Paul Eckhardt,Birthe Sandargo,Lasse van Geelen,Sherif S Ebada,Till Opatz,Abdel Nasser B Singab,Rainer Kalscheuer

Journal

Beilstein Archives

Published Date

2024/4/15

Chemical investigation of the ethyl acetate extract of the fungus Acremonium sp., derived from the inner tissue of anise roots, led to the isolation and characterization of one new chlorinated compound, acremochlorin O (1), together with five related derivatives (2–6) and an alkaloidal metabolite, ilicicolin H (7). Structure elucidation of the isolated compounds was determined based on extensive 1D/2D NMR spectroscopic analyses and HR-ESI-MS measurements. The absolute configuration of acremochlorin O (1) was established using an electronic circular dichroism (ECD) experiment. All isolated compounds were evaluated for their antibacterial activity against Staphylococcus aureus, Escherichia coli and Mycobacterium tuberculosis, where several compounds revealed potent activities against tested Gram-positive strains.

Role of Egyptian Essential Oils in Health Care and Industry

Authors

Hagar A Sobhy,Ahmed E Elissawy,Omayma A Eldahshan,Abdel Nasser B Singab

Published Date

2024

Essential oils – also known as volatile oils or essences - represent a cornerstone in natural product chemistry. Throughout written human history essential oils have established their specific character as nature’s gift to mankind providing solutions to different problems related to healthcare, agriculture, and industry, among others. Egyptian civilization, lasting from 3300 to 525 BC, had a well-established medical system, in which essential oils played a major role not only confined to the medical use represented by aromatherapy but also extending to the different aspects of life including their ritual ceremonies, personal care, mummification, and others. This book chapter provides a comprehensive overview of the different applications of essential oils from the Egyptian flora, bridging the historical gap from the old uses as recorded in the medical papyri to the most recent applications in the pharmaceutical industry …

Unearthing the Potential Fungal Bioactive Secondary Metabolites from the Red Sea: A Comprehensive Review

Authors

Ahmed M Elissawy,Abdel-Nasser Singab

Published Date

2023/9/21

The Red Sea represents a potential ecosystem with very rich biodiversity in the production of unique natural compounds. Recently, fungal secondary metabolites had been a major domain of research in the meadow of natural compounds with special emphasis on fungal strains isolated from marine ecosystems. More than 150 interesting secondary metabolites going with different classes of natural compounds had been reported with potential biological actions as cytotoxic activity with emphasis to the application of different approaches for example OSMAC ( one strain many compounds ) approach. The current review presents a comprehensive report for fungal secondary metabolites isolated from the Red Sea ecosystems either as endophytes from marine invertebrates (for example corals and sponges) and plants for example (algae and mangrove) or as free living strains collected from sea sediments from different locations of Red Sea. Different databases had been utilized including the EKB (Egyptian Knowledge Bank), SciFinder and MarinLit.. In no doubt, the richness of published data directs to the marine fungi's medicinal potential. It will continue to be challenging to incorporate marine fungus into traditional therapy. The clinical environment demonstration of therapeutic activity is a fundamental task for medicine research. For the profit of patients all through the world, it is to be wished that this significant barrier will be removed in the years to arise.

Protective effect of acrocarpus fraxinifolius extract against hepatic fibrosis induced by Gamma irradiation and carbon tetrachloride in albino rats

Authors

Omama E El-Shawi,Heba AS El-Nashar,Sahar S Abd El-Rahman,Omayma A Eldahshan,Abdel Nasser B Singab

Journal

International Journal of Radiation Biology

Published Date

2023/2/1

PurposeLiver fibrosis is considered as one of the ultimate outcomes of chronic liver disorders, characterized by outrageous cell proliferation and abnormal deposition of extracellular matrix, resulting in sever pathological distortions in the architecture and performance of liver tissues. The present study aimed to investigate the protective properties of aqueous methanol extract of Acrocarpus fraxinifolius leaves (AFL) against liver fibrosis induced by dual toxicity of γ-irradiation and carbon tetrachloride (CCl4) in rats.MethodsThe animals were exposed to 2 Gy irradiation once/week concurrently with intraperitoneal administration of CCl4 (0.2 mL/100 g body weight) for seven weeks. Afterwards, liver toxicity and fibrosis were assessed biochemically at cellular and molecular as well as histopathological levels.ResultsThe livers of intoxicated rats showed distinct structural and functional changes, compared with the …

Metabolic profiling, antioxidant, and enzyme inhibition potential of Iris pseudacorus L. from Egypt and Japan: A comparative study

Authors

Suzan M Yehia,Iriny M Ayoub,Masato Watanabe,Hari Prasad Devkota,Abdel Nasser B Singab

Journal

Scientific Reports

Published Date

2023/3/30

Genus Iris comprises numerous and diverse phytoconstituents displaying marked biological activities. The rhizomes, and aerial parts of Iris pseudacorus L. cultivars from Egypt and Japan were subjected to comparative metabolic profiling using UPLC-ESI-MS/MS. The antioxidant capacity was determined using DPPH assay. In vitro enzyme inhibition potential against α-glucosidase, tyrosinase and lipase was evaluated. In silico molecular docking was conducted on the active sites of human α-glucosidase and human pancreatic lipase. Forty-three compounds were tentatively identified including flavonoids, isoflavonoids, phenolics and xanthones. I. pseudacorus rhizomes extracts (IPR-J and IPR-E) exhibited the highest radical scavenging activity with IC50 values of 40.89 µg/mL and 97.97 µg/mL, respectively (Trolox IC50 value was 14.59 µg/mL). Moreover, IPR-J and IPR-E exhibited promising α-glucosidase …

Erythrina Alkaloids: An Updated Review with Neurological Perspective.

Authors

Ahmed Salem,Nada M Mostafa,Eman Al-Sayed,Abdel-Nasser Singab

Published Date

2023/6/1

Erythrina is a genus comprising several plant species that are distributed in tropical and subtropical regions. This genus is a source of many phytoconstituents, with alkaloids and flavonoids as the main bioactive components. Erythrina alkaloids are characterized by a unique nucleus. One hundred ninety-eight alkaloids were isolated from Erythrina plants till early 2023. Recently, an increase in the number of isolated dimeric alkaloids has been witnessed. Erythrina plants have been used in the management of different conditions as oxidative stress and diseases like cancer, inflammation, viral diseases, malaria, and diabetes, with the alkaloids showing a good potential against certain neurological disorders like anxiety, epilepsy, and Alzheimer’s disease. All data on Erythrina alkaloids were collected from Google Scholar, ScienceDirect, SCOPUS, SciFinder. For compounds isolated from multiple Erythrina species, the plant source was traced only from 2008 till 2023. The reported anxiolytic, anti-convulsant, anti-Alzheimer’s activities of Erythrina plant metabolites were traced from 2002 till 2023.

Phytoecdysteroids and Anabolic Effect of Atriplex dimorphostegia: UPLC-PDA-MS/MS Profiling, In Silico and In Vivo Models

Authors

Eman Zaghloul,Heba Handousa,Abdel Nasser B Singab,Mohey M Elmazar,Iriny M Ayoub,Noha Swilam

Journal

Plants

Published Date

2023/1/3

Atriplex dimorphostegia (Saltbush) is an annual halophytic shrub that is widely distributed across various parts of Asia. The current study is the first to report the metabolites profile of the total ethanol extract of the aerial parts of A. dimorphostegia (TEAD), and its anabolic activity together with the isolated 20-hydroxyecdysone (20-HE) in orchidectomized male rats. TEAD was analyzed and standardized utilizing UPLC-PDA-ESI–MS/MS and UPLC-PDA-UV techniques, resulting in tentative identification of fifty compounds including polyphenols, steroids and triterpenoids. In addition, 20-HE was quantified, representing 26.79 μg/mg of the extract. Phytochemical investigation of TEAD resulted in the isolation of 20-HE from the ethyl acetate fraction (EFAD) and was identified by conventional spectroscopic methods of analysis. Furthermore, the anabolic effect of the isolated 20-HE and TEAD was then evaluated using in silico and in vivo models. Molecular docking experiments revealed in vitro selectivity of 20-HE towards estrogen receptors (ERs), specifically ERβ over ERα and androgenic receptor (AR). The anabolic efficacy of TEAD and 20-HE was studied in orchidectomized immature male Wistar rats using the weight of gastrocnemius and soleus muscles. The weights of ventral prostate and seminal vesicles were used as indicators for androgenic activity. Rats administered 20-HE and TEAD showed a significant increase (p = 0.0006 and p < 0.0001) in the net muscle mass compared to the negative control, while the group receiving TEAD showed the highest percentage among all groups at p < 0.0001. Histopathological investigation of skeletal muscle …

Eremophila purpurascens: Anti-oxidant, Anti-hyperglycemic, and Hepatoprotective Potential of Its Polyphenolic Rich Leaf Extract and Its LC–ESI–MS/MS Chemical …

Authors

Fadia S Youssef,Mariam I Gamal El-Din,Hesham A El-Beshbishy,Mohamed L Ashour,Abdel-Nasser B Singab

Journal

ACS omega

Published Date

2023/8/24

The genus Eremophila, despite comprising more than 250 species, has scarce literature studies that could be traced concerning the chemical profile and bioactivity of Eremophila purpurascens. The current study targets the investigation of the in vitro and in vivo anti-oxidant, anti-hyperglycemic, and hepatoprotective potential of the polyphenol-rich leaf extract of E. purpurascens (EP). EP showed promising total anti-oxidant capacity with IC50 values of 106 and 114 μg/mL in 2,2′-azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS) and diphenyl-1-picrylhydrazyl (DPPH) assays, respectively, with total anti-oxidant capacities of 331, 245, and 1767 μmol/g in ABTS, DPPH, and ferric reducing anti-oxidant power assays, respectively. In HepG2 cells, pre-treated with CCl4, a dose of 100 μg/mL EP ameliorated the reduced superoxide dismutase and glutathione levels and total anti-oxidant capacity …

Synergistic Effect of Sophora japonica and Glycyrrhiza glabra Flavonoid-Rich Fractions on Wound Healing: In Vivo and Molecular Docking Studies

Authors

Shaza H Aly,Ahmed M Elissawy,Abdulla MA Mahmoud,Fatma Sa’eed El-Tokhy,Sherif S Abdel Mageed,Hadia Almahli,Sara T Al-Rashood,Faizah A Binjubair,Mahmoud A El Hassab,Wagdy M Eldehna,Abd El-Nasser B Singab

Journal

Molecules

Published Date

2023/3/27

Glycyrrhiza glabra and Sophora japonica (Fabaceae) are well-known medicinal plants with valuable secondary metabolites and pharmacological properties. The flavonoid-rich fractions of G. glabra roots and S. japonica leaves were prepared using Diaion column chromatography, and the confirmation of flavonoid richness was confirmed using UPLC-ESI-MS profiling and total phenolics and flavonoids assays. UPLC-ESI-MS profiling of the flavonoid-rich fraction of G. glabra roots and S. japonica leaves resulted in the tentative identification of 32 and 23 compounds, respectively. Additionally, the wound healing potential of topical preparations of each fraction, individually and in combination (1:1) ointment and gel preparations, were investigated in vivo, supported by histopathological examinations and biomarker evaluations, as well as molecular docking studies for the major constituents. The topical application of G. glabra ointment and gel, S. japonica ointment and gel and combination preparations significantly increase the wound healing rate and the reduction of oxidative stress in the wound area via MDA reduction and the elevation of reduced GSH and SOD levels as compared to the wound and Nolaver®-treated groups. The molecular docking study revealed that that major compounds in G. glabra and S. japonica can efficiently bind to the active sites of three proteins related to wound healing: glycogen synthase kinase 3-β (GSK3-β), matrix metalloproteinases-8 (MMP-8) and nitric oxide synthase (iNOS). Consequently, G. glabra roots and S. japonica leaves may be a rich source of bioactive metabolites with antioxidant, anti-inflammatory and …

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Abdel Nasser B.Singab FAQs

What is Abdel Nasser B.Singab's h-index at Ain Shams University?

The h-index of Abdel Nasser B.Singab has been 36 since 2020 and 41 in total.

What are Abdel Nasser B.Singab's top articles?

The articles with the titles of

The impact of seasonal variation on the composition of the volatile oil of Polyalthia suberosa (Roxb.) Thwaites leaves and evaluation of its acetylcholinesterase …

Comparative metabolic study of the chloroform fraction of three Cystoseira species based on UPLC/ESI/MS analysis and biological activities

Dehydrocostus lactone from the root of Ajuga integrifolia (Buch.-Ham. Ex D. Don): Quantitative determination and in-silico study for anti-breast cancer activity

Unveiling the Antimicrobial and Larvicidal Potential of Butyrolactones and Orsellinic Acid Derivatives from the Morus alba‐derived Fungus Aspergillus terreus via Integrated In …

Benzohydrazide as a good precursor for the synthesis of novel bioactive and anti-oxidant 2-phenyl-1, 3, 4-oxadiazol-aminoacid derivatives: Structural determination, biological …

Medicinal and aromatic plants-I

Role of Nutraceuticals in Obesity Management: A Mechanism and Prospective Supported by Molecular Docking Studies

Unearthing the Potential Secondary Metabolites Isolated from the Fungus Aspergillus flocculosus: A Comprehensive Review.

...

are the top articles of Abdel Nasser B.Singab at Ain Shams University.

What are Abdel Nasser B.Singab's research interests?

The research interests of Abdel Nasser B.Singab are: Phytochemistry, hepatoprotective effects of natural products, Pharmacognosy, Phytotherapy

What is Abdel Nasser B.Singab's total number of citations?

Abdel Nasser B.Singab has 5,740 citations in total.

What are the co-authors of Abdel Nasser B.Singab?

The co-authors of Abdel Nasser B.Singab are Prof. Dr. Michael Wink, Chiara Dall'Asta, Shigang Chen, Fadia Salah Youssef, Eman Kamal Al Sayed.

    Co-Authors

    H-index: 114
    Prof. Dr. Michael Wink

    Prof. Dr. Michael Wink

    Ruprecht-Karls-Universität Heidelberg

    H-index: 56
    Chiara Dall'Asta

    Chiara Dall'Asta

    Università degli Studi di Parma

    H-index: 54
    Shigang Chen

    Shigang Chen

    University of Florida

    H-index: 29
    Fadia Salah Youssef

    Fadia Salah Youssef

    Ain Shams University

    H-index: 26
    Eman Kamal Al Sayed

    Eman Kamal Al Sayed

    Ain Shams University

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