Alex Fernando Almeida

Alex Fernando Almeida

Universidade Federal do Tocantins

H-index: 15

Latin America-Brazil

About Alex Fernando Almeida

Alex Fernando Almeida, With an exceptional h-index of 15 and a recent h-index of 13 (since 2020), a distinguished researcher at Universidade Federal do Tocantins, specializes in the field of Microbiologia, Fermentação, Biotecnologia, Enzimas.

His recent articles reflect a diverse array of research interests and contributions to the field:

CROSS-LINKED WHOLE CELLS FOR THE SUCROSE TRANSFRUCTOSYLATION REACTION IN A CONTINUOUS REACTOR: Original scientific paper

Malnutrition Risk In Gastrointestinal Cancer Patients And Its Association With Disease Burden

Use of enzymes in the food industry: A review

Enhancement of fructosyltransferase stability by immobilization on polyhydroxybutyrate and glutaraldehyde‐activated polyhydroxybutyrate for fructooligosaccharides production

Stabilization of β-Galactosidase Encapsulated in Pectin-Alginate Hydrogel and Hydrolysis of Whey Lactose and Whole Milk

CARACTERIZAÇÃO FÍSICO-QUÍMICA DA FARINHA DE BACURI (PLANTONIA INSIGNIS, MART)

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

PARAMETERS FOR THE IMMOBILIZATION OF A LIPASE PRODUCED BY AN AMAZONIAN FILAMENTOUS FUNGUS ON A HYDROPHOBIC SUPPORT ACTIVATED WITH GLUTARALDEHYDE

Alex Fernando Almeida Information

University

Universidade Federal do Tocantins

Position

___

Citations(all)

645

Citations(since 2020)

462

Cited By

305

hIndex(all)

15

hIndex(since 2020)

13

i10Index(all)

18

i10Index(since 2020)

16

Email

University Profile Page

Universidade Federal do Tocantins

Alex Fernando Almeida Skills & Research Interests

Microbiologia

Fermentação

Biotecnologia

Enzimas

Top articles of Alex Fernando Almeida

CROSS-LINKED WHOLE CELLS FOR THE SUCROSE TRANSFRUCTOSYLATION REACTION IN A CONTINUOUS REACTOR: Original scientific paper

Authors

Beatriz Menossi Ribeiro,Leandro da Rin de Sandre Junior,Giancarlo de Souza Dias,Michelle da Cunha Abreu Xavier,Alex Fernando De Almeida,Elda Sabino Da Silva,Alfredo Eduardo Maiorano,Rafael Firmani Perna,Sergio Andres Villalba Morales

Journal

Chemical Industry & Chemical Engineering Quarterly

Published Date

2024

Fructooligosaccharides (FOS) are fructose oligomers beneficial to human health and nutrition for prebiotic sugars. Their production occurs by a transfructosylation reaction in sucrose molecules catalyzed by fructosyltransferase enzymes (FTase, EC 2.4. 1.9) adhered to microbial cells. The purpose of this work was to study the preparation, enzymatic activity, and stability of glutaraldehyde-crosslinked Aspergillus oryzae IPT-301 cells used as a biocatalyst for the transfructosylation reaction of sucrose in a packed bed reactor (PBR), aiming at FOS production. The highest transfructosylation activity (A T) was presented by the biocatalyst prepared by cross-linking at 200 rpm and 45 min. The highest A T in the PBR was obtained at 50 C, with flow rates from 3 mL min-1 to 5 mL min-1 and sucrose concentrations of 473 g L-1 and 500 g L-1. The enzymatic kinetics was described using the Michaelis-Menten model. Finally, the biocatalyst showed constant A T of approximately 75 U g-1 and 300 U g-1 for 12 h of reaction in the PBR operating in continuous and discontinuous flow, respectively. These results demonstrate a high potential of glutaraldehyde-crosslinked A. oryzae IPT-301 cells as heterogeneous biocatalysts for the continuous production of FOS in PBR reactors.

Malnutrition Risk In Gastrointestinal Cancer Patients And Its Association With Disease Burden

Authors

CA Santos,C Santos,A Almeida

Journal

Clinical Nutrition ESPEN

Published Date

2023/4/1

Methods: Observational and retrospective study using records from oncology ward patients with at least one inpatient stay. Included age, gender, cancer type, admission length, and nutritional risk assessed with the NRS questionnaireResults: Data gathered from 81 patients,(60, 5% male) with a median age of 70 years. Four-thirds (75%) had solid cancers, the most prevalent being colorectal (25%). On average 66% had metastization. Mean length of admission was 13, 4 days, higher lengths for solid cancers (14, 38 vs 11 days for hematologic, p< 10%), with a weak relationship to mortality (pearson correlation 15%). Most patients (58%) died from the pathology (64% in solid cancers, 35% in hematologic, p< 1%). Over half of the sample presented mild disease burden (54, 3%). Admission was longer for moderate disease burden (14, 6 days) and shorter for severe (10 days) due to increased mortality. Mean NRS was …

Use of enzymes in the food industry: A review

Authors

Joyce Fagundes Gomes Motta,Bárbara Catarina Bastos de FREITAS,Alex Fernando de ALMEIDA,Glêndara Aparecida de Souza MARTINS,Soraia Vilela BORGES

Published Date

2023/3/27

Enzymes are biological catalysts that play a key role in the food industry, responsible for desirable and undesirable chemical reactions. These compounds can occur spontaneously in food products or even be incorporated on purpose. The main enzyme classes (oxidoreductase, transferase, hydrolase, lyase, isomerase, and ligase) and their subclasses can be obtained from several sources, presenting numerous applications in foods such as inhibition of microorganisms, insertion of aromas, improvement of physico-chemical properties, decreased candy crystallization, meat tenderization, antioxidants, indicators of heat treatment, and so on. Each enzyme acts effectively under specific conditions of pH, temperature, concentration, and water activity. Enzymatic immobilization techniques have been studied to minimize adverse conditions that enzymes are subjected to during food processing. Among the immobilization technique, the enzymes can be immobilized to the packages, being known as enzymatic active packages, the packages allow the enzymes to be migrated to the product or trapped, increasing the shelf life of the products, this technique being innovative both for the packaging market and for the enzyme market. Thus, the main enzymes and their applications in food were briefly discussed in this review article, as well as the main techniques of immobilization and insertion of these compounds in active packaging.

Enhancement of fructosyltransferase stability by immobilization on polyhydroxybutyrate and glutaraldehyde‐activated polyhydroxybutyrate for fructooligosaccharides production

Authors

Isabella Medeiros Araújo,Pedro Capalbo Becalette,Elda Sabino da Silva,Giancarlo de Souza Dias,Michelle da Cunha Abreu Xavier,Alex Fernando de Almeida,Alfredo Eduardo Maiorano,Sergio Andres Villalba Morales,Rafael Firmani Perna

Journal

Journal of Chemical Technology & Biotechnology

Published Date

2023/2

BACKGROUND Fructosyltransferase (FTase) enzyme is an important biocatalyst for fructooligossaccaride (FOS) production, but it has low stability in soluble form. The aim of this work was to analyze the stability and kinetics of an fructosyltransferase (FTase E.C.2.4.1.9) from Aspergillus oryzae IPT‐301 immobilized on polyhydroxybutyrate (PHB) and glutaraldehyde‐activated polyhydroxybutyrate (GLU‐PHB). RESULTS The immobilization yields of the FTase on PHB and GLU‐PHB were about 41% and 55%, respectively. The recovery activities of the FTase immobilized on PHB and GLU‐PHB were about 17% and 11%, respectively. At 30 °C, the FTase immobilized on GLU‐PHB showed a half‐life 2.6 and 1.4 times higher than the soluble FTase and the FTase immobilized on PHB, respectively. The FTase immobilized on PHB and GLU‐PHB retained more than 40% and 55% of their initial activity after six …

Stabilization of β-Galactosidase Encapsulated in Pectin-Alginate Hydrogel and Hydrolysis of Whey Lactose and Whole Milk

Authors

Maykon J Paiva,Fabrício C Paula-Elias,Luciana A Pereira,Solange C Carreiro,Erika C Vieira-Almeida,Ezequiel M Silva,Giancarlo S Dias,Michelle AC Xavier,Sergio A Morales,Rafael F Perna,Alex F Almeida

Journal

Journal of the Brazilian Chemical Society

Published Date

2023/11/27

The operational conditions of β-galactosidase encapsulated in pectin-alginate hydrogel were investigated as well as its catalytic activity in the hydrolysis of lactose from whey and whole milk. β-Galactosidase encapsulated in pectin-alginate hydrogel showed the best results to yields and diffusional effect and pH range. Thermostability showed considerable income gain for half-life (1.7-fold), activation energy of denaturation (31.6-fold), activation of denaturation (217.3-fold at 40 ºC; 266.8-fold at 50 ºC; 345.6-fold at 60 ºC), and entropy of activation of denaturation (-42.0 J mol 1 K-1). Reusability of encapsulated β-galactosidase was observed in 8-cycles for the milk whey lactose hydrolysis (51.9%) and 7-cycles for whole milk (55.6%), and the lactose hydrolysis was observed in 13% in milk whey and 10.3% in whole milk after 10 cycles. These results revealed an industrial application potential of β-galactosidase encapsulated in pectin-alginate hydrogel for lactose hydrolysis of milk whey and whole milk processes.

CARACTERIZAÇÃO FÍSICO-QUÍMICA DA FARINHA DE BACURI (PLANTONIA INSIGNIS, MART)

Authors

Cecília Marques Tenório PEREIRA,Catharina do Couto Caetano COSTA,Caroline Roberta Freitas PIRES,Alex Fernando de ALMEIDA

Published Date

2023

O processamento da farinha através do método de secagem ajuda a reduzir o desperdício de resíduos ricos em nutrientes, além de promover a qualidade nutricional do produto. O Bacuri (Platonia insignis Mart) possui compostos bioativos que conferem características sensoriais importantes. O objetivo deste trabalho foi avaliar as características físico-químicas e antioxidantes da farinha do Bacuri. A farinha foi obtida pela secagem do endocarpo em estufa, à 90 C, e foram realizadas análises de composição nutricional, pH, acidez titulável, cor, vitamina C, carotenoides, compostos fenólicos, atividade antioxidante pelo método de ABTS+ e FRAP. Apresentou altos teores de fibra (13, 7%), carboidrato (71, 11%), e pode ser considerado um alimento de alto valor energético (349, 05%). Com pH de 3, 24, indica alta acidez, uma vez que alimentos com pH< 4, 5 são considerados muito ácidos, além de contribuir para …

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

Authors

Lucely Nogueira Dos Santos,Rafael Firmani Perna,Ana Carolina Vieira,Alex Fernando de Almeida,Nelson Rosa Ferreira

Published Date

2023/8/15

Scientific mapping using bibliometric data network analysis was applied to analyze research works related to lipases and their industrial applications, evaluating the current state of research, challenges, and opportunities in the use of these biocatalysts, based on the evaluation of a large number of publications on the topic, allowing a comprehensive systematic data analysis, which had not yet been conducted in relation to studies specifically covering lipases and their industrial applications. Thus, studies involving lipase enzymes published from 2018 to 2022 were accessed from the Web of Science database. The extracted records result in the analysis of terms of bibliographic compatibility among the articles, co-occurrence of keywords, and co-citation of journals using the VOSviewer algorithm in the construction of bibliometric maps. This systematic review analysis of 357 documents, including original and review articles, revealed studies inspired by lipase enzymes in the research period, showing that the development of research, together with different areas of knowledge, presents good results related to the applications of lipases, due to information synchronization. Furthermore, this review showed the main challenges in lipase applications regarding increased production and operational stability; establishing well-defined evaluation criteria, such as cultivation conditions, activity, biocatalyst stability, type of support and reactor; thermodynamic studies; reuse cycles; and it can assist in defining goals for the development of successful large-scale applications, showing several points for improvement of future studies on lipase enzymes.

PARAMETERS FOR THE IMMOBILIZATION OF A LIPASE PRODUCED BY AN AMAZONIAN FILAMENTOUS FUNGUS ON A HYDROPHOBIC SUPPORT ACTIVATED WITH GLUTARALDEHYDE

Authors

Lucely Nogueira dos SANTOS,Amanda Pietra Santerio CAVINI,Gustavo Aparecido Nagae TEIXEIRA,Ana Clara Miranda BATISTA,Kalebe Ferreira FURTADO,Ana Carolina VIEIRA,Rafael Firmani PERNA,Alex Fernando de ALMEIDA,Nelson ROSA

Published Date

2023

Immobilization positively affects enzyme stability, since the immobilized lipase exhibits higher activity compared to the soluble lipase. This work aimed to immobilize a lipase from an Amazonian filamentous fungus isolated from bacaba (Oenocarpus bacaba), a typical Amazonian fruit, on glutaraldehyde-activated polyhydroxybutyrate (GLU-PHB) for further essential fatty acids production. The soluble enzyme was produced by submerged cell cultivation in an orbital shaker at 30 C and 200 rpm for 168 h, inoculating 1x10^ 7 spores/mL in a synthetic culture medium (pH 5.5) formulated with 5.5 g/L of K2HPO4, 15 g/L of KHPO4, 0.5 g/L of MgSO4. 7H2O, 1.0%(m/v) of olive oil and 0.2% of yeast extract (m/v). The crude extract was used for the immobilization assays. PHB particles of 1.2 mm were washed with 95%(v/v) ethanol pa at the proportion of 1: 10 (w/v) at 120 rpm and 30 ºC for 6 h. Subsequently, the biopolymer was …

Selection and validation of reference genes for RT-qPCR gene expression studies in Candida viswanathii cultivated under different grown conditions

Authors

Dayoung Oh,Ward De Spiegelaere,Hans J Nauwynck

Journal

Scientific Reports

Published Date

2023/5/31

Porcine alveolar macrophages (PAMs) are widely used for in vitro studies of porcine respiratory viruses. Gene expression in these cells is altered by viral infection and cellular immune response. Real-time reverse transcription polymerase chain reaction (RT-qPCR) is a powerful technique for analyzing these changes. In order to obtain reliable quantitative RT-qPCR data and come to sound conclusions, stable reference genes are needed for normalization of target gene expression. In the present study, we evaluated the expression stability of nine reference genes in PAMs during cultivation and upon porcine reproductive and respiratory syndrome virus (PRRSV) inoculation. Using geNorm and NormFinder algorithms, we identified PSAP and GAPDH as the most stable reference genes under all experimental conditions. The selected reference genes were used for the normalization of CD163 expression under …

Imobilização de invertase bacteriana em polihidroxibutirato para a produção de açúcar invertido

Authors

Larissa Santos SARAIVA,Natanael do Carmo Sousa GOMES,Pedro Henrique César MORAIS,Érica Daniely SANTOS,Rafaela Rocha PINTO,Rafael Firmani PERNA,Alex Fernando de ALMEIDA,Michelle Cunha Abreu XAVIER,Sergio Andres Vilalba MORALES

Published Date

2023

A enzima invertase catalisa a reação de conversão da sacarose para produção do açúcar invertido, o qual é de alta importância para a indústria de alimentos, pois possui elevado grau de doçura e não cristaliza durante o processamento. Recentemente, microrganismos produtores de invertase foram descobertos em espécies frutíferas do bioma amazônico, dentre elas a pupunha (Bactris gasipaes). Porém, a invertase microbiana em sua forma livre apresenta baixa estabilidade e não pode ser reutilizada, limitando a sua aplicação na produção do açúcar invertido. Este trabalho objetivou avaliar a imobilização, por adsorção física e ligação covalente, da invertase produzida por Bacillus tequilensis, isolado da pupunha, em polihidroxibutirato (PHB) e PHB funcionalizado com glutaraldeído, visando a obtenção de biocatalisadores heterogêneos estáveis para a reação de hidrólise da sacarose. Para isso, o PHB foi …

Crescimento, demanda agregada e distribuição pessoal da renda no Brasil: 1996 a 2015

Authors

Alex Almeida,Douglas Alencar

Journal

Brazilian Keynesian Review

Published Date

2022/10/3

Considering the Post-Keynesian economic growth literature, the demand regimes emerge as an important factor. The demand regimes explore the impact of changes in the functional income distribution on economic growth. In the case of redistribution of income from profits to wages promotes economic growth, it’s said the economy is wage-led, in the opposite case, the economy is profit-led. This research estimates a Vector Autoregression (VAR) from 1996 to 2015 to investigate if the Brazilian demand regime can be considered wage-led or profit-led. Furthermore, it is included the personal income distribution with the purpose to avoid a possible omitted variable bias arising for ignoring the effects that a changing on that distribution can has over demand regimes.

Study on the transfructosylation activity of Aspergillus oryzae IPT‐301 cells in a packed bed reactor aiming at fructooligosaccharide production

Authors

Giancarlo S Dias,Erica D Santos,Michelle CA Xavier,Alex F Almeida,Elda S Silva,Alfredo E Maiorano,Rafael F Perna,Sergio AV Morales

Journal

Journal of Chemical Technology & Biotechnology

Published Date

2022/10

BACKGROUND Fructooligosaccharides (FOS) are prebiotic sugars used in the production of functional foods. These sugars can be produced by the transfructosylation reaction of sucrose catalyzed by fructosyltransferase enzymes (FTase, E.C.2.4.1.9). The aim of this work was to evaluate the use of cells of Aspergillus oryzae IPT‐301 as biocatalysts for the sucrose transfructosylation reaction in a packed bed reactor (PBR). RESULTS The morphology of the cells was observed by scanning electron microscopy, and BET specific surface areas were examined by nitrogen physisorption The highest transfructosylation activity (660 U g−1) of the cells was obtained at 25 min of reaction by adopting a reaction temperature of 50 °C, sucrose concentration of 470 g L−1, and volumetric flow of 15 mL min−1. The rise in volumetric flow provided an increase in the transfructosylation activity, but also a faster deactivation …

Lipase from Fusarium solani: optimization of culture conditions, biochemical properties, and production

Authors

Danylo Bezerra Mendes,Fabiane Fernandes da Silva,Patricia Martins Guarda,Alex Fernando de Almeida,Emerson Adriano Guarda

Journal

Research, Society and Development

Published Date

2022/8/19

A Plackett-Burman factorial design with 15 experiments was conducted to evaluate the influence of seven factors on lipases production by Fusarium solani. The factors investigated were peptone, tryptone, yeast extract, calcium chloride, potassium phosphate, magnesium sulfate, and copper sulphate. Five fixed variables (cotton oil, pH, temperature, agitation, and time) were maintained and as a response to the enzymatic activity. The concentration of tryptone, calcium chloride, and magnesium sulphate had a significant effect (p< 0.10) on lipase production and was studied consecutively through a complete DCCR (central rotational compound design), to optimize lipase production of the fungi F. solani. After optimization using DCCR, maximum lipolytic activities of 24.84 U/ml were obtained with the use of 10 gL-1 tryptone, 3.50 gL-1 calcium chloride and 0.50 gL-1 magnesium sulfate, 1 gL-1 potassium phosphate and 1% soybean oil. The statistical model showed a correlation of 85.67% with the experimental data. The biochemical characterization of lipase showed that the enzyme has a better performance at pH 7 at a temperature of 40 C, where the statistical model had a correlation of 94.15% with the experimental data. In this way, lipases produced by F. solani have potential for application and use in biodiesel production.

Performance of wild Saccharomyces and Non‐Saccharomyces yeasts as starter cultures in dough fermentation and bread making

Authors

Bárbara Marques Bianchini Condessa,Kamila Veloso da Silva,Juliana Fonseca Moreira da Silva,Paula Benevides de Morais,Fernanda MA Leal Zimmer,Alex Fernando de Almeida,Edenilson dos Santos Niculau,Karolina Lima Nogueira,Claudia Cristina Auler do Amaral Santos

Journal

International Journal of Food Science & Technology

Published Date

2022/5

The use of wild Saccharomyces and non‐Saccharomyces yeasts might result in bread with different and attractive sensory characteristics. This study aimed to evaluate the performance of Saccharomyces and non‐Saccharomyces yeasts as starter culture in dough fermentation to bread making and the physicochemical parameters and aromatic profile of bread. All 26 wild yeasts strains isolated from Brazilian Cerrado fruit and tree bark were osmotolerant, and 19.4% were able to ferment maltose. Candida tropicalis ART101.3 and Saccharomyces cerevisiae SC5952 had the best growth capacity under high concentrations of glucose and maltose. Also, they were resistant to lyophilisation. Kinetic parameters of bioreactor cultivations showed high cell growth and lower generation time with 10 g L−1 maltose. Bread produced with C. tropicalis ART101.3 and the control bread had similar physicochemical properties …

Simultaneous high nutritional single cell oil and lipase production by Candida viswanathii

Authors

Kleydiane B Dias,Nayra ML Oliveira,Bruno SAF Brasil,Erika C Vieira-Almeida,Fabrício C Paula-Elias,Alex F Almeida

Journal

Acta Scientiarum Polonorum Technologia Alimentaria

Published Date

2021/3/30

Background. Omega fatty acids are a family of polyunsaturated fats associated with several health benefits. Lipases are enzymes with potential application in several food processes such as flavor and aroma, surfactants and formulations for the dairy and bakery industries. In this study, single cell oil and lipase production by Candida viswanathii CCR8137 were evaluated simultaneously from renewable carbon sources under nitrogen limitation.Materials and methods. Enzyme and single cell oil were obtained in submerged cultivations supplemented with triolein, tributyrin, corn oil, sunflower oil, canola oil and olive oil. The effects of glucose on lipid accumulation, fatty acid profile, enzyme production and cell morphology were also evaluated.Results. The highest lipid accumulation (44.5%, w/w) was obtained from triolein, whereas olive oil was the best inducer of lipase synthesis (26.8 U/mL). Nitrogen limiting cultivations were a key parameter for an organic source which showed higher lipid accumulation and enzyme production than the tested inorganic nitrogen source. Glucose was a poor inducer of lipase synthesis, though increased values of lipid accumulation were observed from this carbon source with a maximum of 63.1%(w/w). The fatty acid profile of lipids produced by C. viswanathii CCR8137 showed a high content of omega-9 fatty acid (C18: 1 n-9). The addition of glucose to the culture media resulted in the synthesis of essential fatty acids: vaccenic, linolenic and eicosadienoic acids.Conclusion. Therefore, C. viswanathii CCR8137 strain can be considered as an oleaginous yeast able to accumulate high concentrations of intracellular lipids …

Effect of co-encapsulation using a calcium alginate matrix and fructooligosaccharides with gelatin coating on the survival of Lactobacillus paracasei cells

Authors

Rosetania Correia Neves da Conceicao,Rayssa Dias Batista,Fernanda Munhoz dos Anjos Leal Zimmer,Ianna Kelly Martins Trindade,Alex Fernando de Almeida,Claudia Cristina Auler do Amaral Santos

Journal

BRAZILIAN JOURNAL OF MICROBIOLOGY

Published Date

2021/9/1

The demand for functional foods is increasing each year because consumers are gaining awareness about the importance of a healthy diet in the proper functioning of the body. Probiotics are among the most commonly known, commercialized, and studied foods. However, the loss of viability of probiotic products is observed during their formulation, processing, and storage. This study aimed to investigate the co-encapsulation of two Lactobacillus paracasei probiotic strains (LBC81 and ELBAL) with fructooligosaccharides (FOS) in a calcium alginate matrix using extrusion technology with gelatin as a coating material. The viability of the strains under gastrointestinal conditions and in storage at low temperature was also assessed. An immobilization yield of more than 59% was observed for both bacterial strains. Exposure to 2% biliary salts led to a decrease in the viability of free cells in the two L. paracasei strains …

Produção de goma xantana por cepa selvagem de Xanthomonas spp. usando soro de leite como substrato na fermentação

Authors

Fabriele de Sousa Ferraz,Ana Clara Alves Braga Rodrigues,Danilo José Machado de Abreu,Roseane Veras de Souza,Alex Fernando de Almeida,Claudia Cristina Auler do Santos

Journal

Scientia Plena

Published Date

2021/9/9

A goma xantana é um biopolímero microbiano de grande importância na indústria alimentícia. A produção desse polissacarídeo é influenciada diretamente pelas condições de cultivo, linhagens e substratos empregados como sacarose e glicose, o que tornam o processo oneroso. A busca por linhagens que apresentem boa relação quantidade/qualidade e por substratos que diminuam o custo de produção, como por exemplo os resíduos agroindustriais, tem sido foco de pesquisas recentes. O presente estudo visou produzir goma xantana utilizando o soro de leite em substituição parcial da sacarose no meio de fermentação, na proporção de 1: 1. Utilizou-se a cepa selvagem de Xanthomonas sp. MN37. 2, isolada de tecidos foliares de mangueira. A Fermentação controle foi conduzida com meio contendo 50g/L de sacarose. As fermentações foram realizadas à 28 C, à 180 rpm por 96 horas. O pH decaiu de 6, 54 para 5, 18 durante o processo fermentativo da cepa e a população de células viáveis aumentou de 10, 15 log UFC/mL para 11, 69 log UFC/mL. O rendimento de produção da goma na fermentação com adição de soro de leite foi de 62, 13% quando comparado à fermentação com sacarose como única fonte de carbono, sendo 30, 35 g/L e 48, 85 g/L, respectivamente. Portanto, o soro de leite demonstra potencial para ser empregado como substrato de baixo custo na obtenção de goma xantana por bactérias do gênero Xanthomonas. Entretanto, estudos de otimização da formulação do meio de fermentação, bem como controle das etapas de recuperação da goma devem ser realizados afim de viabilizar a utilização em escala …

Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept

Authors

Carolina Bilia Chimello de Paula,Fabricio Coutinho de Paula-Elias,Marcela Nogueira Rodrigues,Luciana Fontes Coelho,Nayra Morgana Lima de Oliveira,Alex Fernando de Almeida,Jonas Contiero

Journal

Frontiers in Bioengineering and Biotechnology

Published Date

2021/1/13

Polyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared to pure sucrose, with intracellular polymer accumulation values of 41.1–46.6% cell dry weight. Whereas, xylose and bagasse hydrolysate were poor inducers of microbial growth and polymer synthesis, the addition of 25% (v/v) sugarcane vinasse to the culture media containing molasses was not deleterious and resulted in a statistically similar maximum polymer content of 44.8% and a maximum PHA yield of 0.18 g/g, at 34°C and initial pH of 6.5, which is economic and ecologically interesting to save water required for the industrial processes and especially to offer a fermentative recycling for this final byproduct from bioethanol industry, as an alternative to its inappropriate disposal in water bodies and soil contamination. Ammonium sulfate was better even than tested organic nitrogen sources to trigger the PHA synthesis with polymer content ranging from 29.7 to 44.8%. GC-MS analysis showed a biopolymer constituted mainly of poly(3-hydroxybutyrate) although low fractions of 3-hydroxyvalerate monomer were achieved, which were not higher than 1.5 mol% free of copolymer precursors. B. glumae MA13 has been demonstrated to be adapted to synthesize bioplastics from different sugarcane feedstocks and corroborates to support a …

Effect of temperature, pH and storage time on the stability of an extracellular fructosyltransferase from Aspergillus oryzae IPT-301

Authors

Maria Beatriz Pregnolato de Oliveira Silva,Daniela Abdal,José Pedro Zanetti Prado,Giancarlo de Souza Dias,Sergio Andres Villalba Morales,Michelle da Cunha Abreu Xavier,Alex Fernando de Almeida,Elda Sabino da Silva,Alfredo Eduardo Maiorano,Rafael Firmani Perna

Journal

Brazilian Journal of Food Technology

Published Date

2021/7/16

In this work, it was determined the influence of temperature, pH and storage time on the enzymatic activity and stability of an extracellular fructosyltransferase (FTase E.C.2.4.1.9) from Aspergillus oryzae IPT-301 produced by submerged fermentation. The thermodynamic parameters showed a tendency for increasing enzyme denaturation with the rise in temperature. The maximum transfructosylation activity was obtained at the incubation pH 5.5. During storage at 4 °C, the transfructosylation activity decreased, whereas the hydrolytic activity increased, especially in the first nine hours, a time in which the enzyme presented 45.6% of its initial transfructosylation activity. These results contributed to the improvement of the conditions of storage, immobilization and use of the soluble fructosyltransferases (FTase) in fructooligosaccharide (FOS) production.

Hydrolysis of pineapple crown biomass by cellulolytic enzymes produced by Fusarium oxysporum

Authors

Iara Leandro dos Santos,Gustavo Garcia do Amaral,Alanna Cristinne Martins Lima,Lunara Thaís Alves de Bastos,Annanda Carvalho dos Santos,Fabrício Coutinho de Paula-Elias,Cláudia Cristina Auler do Amaral Santos,Alex Fernando de Almeida

Journal

Scientia Plena

Published Date

2021/9/9

O desenvolvimento de enzimas de origem microbiana é de grande importância nas indústrias de processos bioquímicos. Dentre as enzimas de importância industrial, destacam-se as enzimas celulolíticas e xilanolíticas grandes responsáveis pela degradação do material lignocelulósico que costuma ser descartado pelo setor agroindustrial. Com objetivo de produção das enzimas CMCase, β-glicosidase, xilanase e β-xilosidase, foi utilizado o fungo Fusarium oxysporum em fermentação líquida usando biomassa pré-tratada de coroa de abacaxi como fonte de carbono. Visando otimizar a produção dessas enzimas os cultivos foram realizados por 168 horas, 30 C a 180 rpm. A biomassa passou por um pré-tratamento em duas etapas, sendo a primeira submetida à 121 C por 7 minutos, já à segunda foi acrescentado ácido sulfúrico diluído a 1%, na proporção de 1: 5 (m/v), a 121 C por 27 min. A caracterização parcial do extrato bruto apontou temperatura de 50 ºC como a mais satisfatória para ação dessas enzimas, e a faixa de pH ideal variou de 3.5 a 5.5. As hidrólises enzimáticas de coroa de abacaxi com e sem pré-tratamento, foram realizadas usando extrato bruto liofilizado (CMCase= 9, 78 U/mL, β-glicosidase= 10, 86 U/mL, xilanase= 27, 90 U/mL e β-xilosidase= 0,018 U/mL). Para tal, foi realizado um planejamento experimental de Delineamento Composto Central Rotacional (DCCR). O qual foi possível avaliar o efeito e influência do pH, da concentração de biomassa e tempo de incubação na liberação de glicose no meio (R 2> 0, 85); o pH e a biomassa (%) apresentaram efeito significativo sobre a liberação de glicose.

See List of Professors in Alex Fernando Almeida University(Universidade Federal do Tocantins)

Alex Fernando Almeida FAQs

What is Alex Fernando Almeida's h-index at Universidade Federal do Tocantins?

The h-index of Alex Fernando Almeida has been 13 since 2020 and 15 in total.

What are Alex Fernando Almeida's top articles?

The articles with the titles of

CROSS-LINKED WHOLE CELLS FOR THE SUCROSE TRANSFRUCTOSYLATION REACTION IN A CONTINUOUS REACTOR: Original scientific paper

Malnutrition Risk In Gastrointestinal Cancer Patients And Its Association With Disease Burden

Use of enzymes in the food industry: A review

Enhancement of fructosyltransferase stability by immobilization on polyhydroxybutyrate and glutaraldehyde‐activated polyhydroxybutyrate for fructooligosaccharides production

Stabilization of β-Galactosidase Encapsulated in Pectin-Alginate Hydrogel and Hydrolysis of Whey Lactose and Whole Milk

CARACTERIZAÇÃO FÍSICO-QUÍMICA DA FARINHA DE BACURI (PLANTONIA INSIGNIS, MART)

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

PARAMETERS FOR THE IMMOBILIZATION OF A LIPASE PRODUCED BY AN AMAZONIAN FILAMENTOUS FUNGUS ON A HYDROPHOBIC SUPPORT ACTIVATED WITH GLUTARALDEHYDE

...

are the top articles of Alex Fernando Almeida at Universidade Federal do Tocantins.

What are Alex Fernando Almeida's research interests?

The research interests of Alex Fernando Almeida are: Microbiologia, Fermentação, Biotecnologia, Enzimas

What is Alex Fernando Almeida's total number of citations?

Alex Fernando Almeida has 645 citations in total.

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