abdelkarim abousalham

abdelkarim abousalham

Université Claude Bernard Lyon 1

H-index: 25

Europe-France

Professor Information

University

Université Claude Bernard Lyon 1

Position

Professeur de biochimie

Citations(all)

2076

Citations(since 2020)

723

Cited By

1650

hIndex(all)

25

hIndex(since 2020)

14

i10Index(all)

47

i10Index(since 2020)

20

Email

University Profile Page

Université Claude Bernard Lyon 1

Research & Interests List

Biochimie

enzymologie

lipolyse enzymatique

Top articles of abdelkarim abousalham

Development of a highly efficient oil degumming process using a novel phosphatidylinositol-specific phospholipase C enzyme from Bacillus thuringiensis PL14

The present work reports the heterologous expression and biochemical characterization of a novel phosphatidylinositol phospholipase C from Bacillus thuringiensis PL14 (PI-PLCBt) with potential for oil degumming. As degumming is a necessary refining step for all crude vegetable oils, enzymatic degumming tests were performed on crude soybean oil using rPI-PLCBt. Its efficiency was investigated by determining the phosphorus and diacylglycerol (DAG) contents after different types of treatment. Although the water and chemical degumming process can efficiently reduce the phosphorus content, a specific enzymatic process can still be selected giving its high degumming efficiency of 89.4%, with a noticeable reduction in phosphorus content to 13.7 mg kg−1, close to the values of the chemical approach, and a high increase in DAG. Accordingly, enzymatic degumming could be adopted as an alternative to …

Authors

Ahlem Eddehech,Renaud Rahier,Danilo Donnarumma,Francesca Rigano,Alexandre Noiriel,Abdelkarim Abousalham,Francesco Cacciola,Luigi Mondello,Zied Zarai

Journal

Food Bioscience

Published Date

2023/6/1

Antimicrobial activity of Lactiplantibacillus and Levilactobacillus strains isolated from fermented olive and their application in milk and Pastilla leaf biopreservation.

Biological preservatives are increasingly in demand as an alternative to chemicals, because of consumer's health and environmental concerns. Lactic acid bacteria isolated from fermented olives, considered as extreme environment, may encompass significant antimicrobial activity against microorganisms involved in foodborne diseases and food spoilage. Three LAB strains (Levilactobacillus brevis S27, Lactiplantibacillus pentosus S42, and Lactiplantibacillus plantarum S62), isolated from fermented olive, were studied for their antimicrobial activity against pathogenic bacteria (Listeria innocua and E. coli) and yeast (Rhodotorula glutinis), involved in foodborne diseases and food spoilage. The microbial culture, cell-free supernatant (CFS) of all LAB strains showed inhibition zone (IZ) values ranges of 26.33-28.83 mm, 14.16-16.33 mm and 10-12.16 mm against R. glutinis, L. innocua and E. coli, respectively …

Authors

Ismail Hasnaoui,Houssam Abouloifa,Yahya Rokni,Reda Bellaouchi,Meryem Idrissi Yahyaoui,Ennouamane Saalaoui,Alexandre Noiriel,Abdelkarim Abousalham,Kamal Essifi,Abdesselam Tahani,Bassem Jaouadi,Abdeslam Asehraou

Published Date

2023/5/31

Biochemical characterization of an alkaline and detergent-stable Lipase from Fusarium annulatum Bugnicourt strain CBS associated with olive tree dieback

This work describes a novel extracellular lipolytic carboxylester hydrolase named FAL, with lipase and phospholipase A1 (PLA1) activity, from a newly isolated filamentous fungus Ascomycota CBS strain, identified as Fusarium annulatum Bunigcourt. FAL was purified to about 62-fold using ammonium sulphate precipitation, Superdex® 200 Increase gel filtration and Q-Sepharose Fast Flow columns, with a total yield of 21%. The specific activity of FAL was found to be 3500 U/mg at pH 9 and 40°C and 5000 U/mg at pH 11 and 45°C, on emulsions of triocanoin and egg yolk phosphatidylcholine, respectively. SDS-PAGE and zymography analysis estimated the molecular weight of FAL to be 33 kDa. FAL was shown to be a PLA1 with a regioselectivity to the sn-1 position of surface-coated phospholipids esterified with α-eleostearic acid. FAL is a serine enzyme since its activity on triglycerides and phospholipids was completely inhibited by the lipase inhibitor Orlistat (40 μM). Interestingly, compared to Fusarium graminearum lipase (GZEL) and the Thermomyces lanuginosus lipase (Lipolase®), this novel fungal (phospho)lipase showed extreme tolerance to the presence of non-polar organic solvents, non-ionic and anionic surfactants, and oxidants, in addition to significant compatibility and stability with some available laundry detergents. The analysis of washing performance showed that it has the capability to efficiently eliminate oil-stains. Overall, FAL could be an ideal choice for application in detergents.

Authors

Ahlem Dab,Ismail Hasnaoui,Sondes Mechri,Fawzi Allala,Khelifa Bouacem,Alexandre Noiriel,Amel Bouanane-Darenfed,Ennouamane Saalaoui,Abdeslam Asehraou,Fanghua Wang,Abdelkarim Abousalham,Bassem Jaouadi

Journal

Plos one

Published Date

2023/5/19

Purification, Biochemical and Kinetic Characterization of a Novel Alkaline sn-1,3-Regioselective Triacylglycerol Lipase from Penicillium crustosum Thom Strain …

A novel extracellular lipase from a filamentous fungus Ascomycota strain, P22, was isolated from olive mill wastewater, then purified and characterized. This strain was identified as Penicillium crustosum Thom based on sequencing analyses. Penicillium crustosum Thom strain P22 lipase (PCrL) was purified 63-fold to homogeneity using ammonium sulfate precipitation and chromatography on a Q-Sepharose Fast Flow column, with a total yield of 34%. The purified PCrL had a molecular mass of 28 kDa, estimated by SDS-PAGE. The 20 NH2-terminal amino-acid residues showed a high degree of homology with those of other Penicillium lipases. The specific activity of PCrL at pH 9 and 37 °C were found to be 5000 and 10,000 U/mg on olive oil and trioctanoin emulsions, respectively. PCrL exhibited clear regioselectivity toward the sn-1 position of the surface-coated triglycerides which were esterified with α-eleostearic acid at the sn-1/3 position. PCrL was completely inhibited by 53 µM of Orlistat, 5 mM of phenylmethylsulfonylfluoride, and 2 mM of diiodopropyl fluorophosphate, suggesting that it belonged to the serine lipase family. PCrL showed high activity and stability in the presence of water-immiscible organic solvents, surfactant, and oxidizing agents, and showed considerable compatibility with commercial laundry detergents. Washing performance analysis revealed that it could effectively remove oil stains. Hence, PCrL has several attractive properties that make it a promising potential candidate for detergent formulations.

Authors

Ismail Hasnaoui,Ahlem Dab,Sondes Mechri,Houssam Abouloifa,Ennouamane Saalaoui,Bassem Jaouadi,Alexandre Noiriel,Abdeslam Asehraou,Abdelkarim Abousalham

Journal

International Journal of Molecular Sciences

Published Date

2022/10/7

Antifungal activity of lactic acid bacteria and their application in food biopreservation

Lactic acid bacteria (LAB) are ubiquitous bacteria associated with spontaneous lactic fermentation of vegetables, dairy and meat products. They are generally recognized as safe (GRAS), and they are involved in transformation of probiotic lacto-fermented foods, highly desired for their nutraceutical properties. The antifungal activity is one of the exciting properties of LAB, because of its possible application in food bio-preservation, as alternative to chemical preservatives. Many recent research works have been developed on antifungal activity of LAB, and they demonstrate their capacity to produce various antifungal compounds, (i.e. organic acids, PLA, proteinaceous compounds, peptides, cyclic dipeptides, fatty acids, and other compounds), of different properties (hydrophilic, hydrophobic and amphiphilic). The effectiveness of LAB in controlling spoilage and pathogenic fungi, demonstrated in different agricultural …

Authors

Houssam Abouloifa,Ismail Hasnaoui,Yahya Rokni,Reda Bellaouchi,Nabil Ghabbour,Salwa Karboune,Milena Brasca,Abdelkarim Abousalham,Bassem Jaouadi,Ennouamane Saalaoui,Abdeslam Asehraou

Published Date

2022/1/1

Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis strain TN-X30

We recently described the production of a detergent-biocompatible crude protease from Streptomyces mutabilis strain TN-X30. Here, we describe the purification, characterization, and immobilization of the serine alkaline protease (named SPSM), as well as the cloning, sequencing, and over-expression of its corresponding gene (spSM). Pure enzyme was obtained after ammonium sulphate precipitation followed by heat-treatment and Sephacryl® S-200 column purification. The sequence of the first 26 NH2-terminal residues of SPSM showed a high sequence identity to subtilisin-like serine proteases produced by actinobacteria. The spSM gene was heterologously expressed in Escherichia coli BL21(DE3)pLysS and E. coli BL21-AI™ strains using pTrc99A (rSPSM) and Gateway™ pDEST™ 17 [(His)6-tagged SPSM] vectors, respectively. Results obtained indicated that the (His)6-tagged SPSM showed the highest …

Authors

Sondes Mechri,Fawzi Allala,Khelifa Bouacem,Ismail Hasnaoui,Hassan Gwaithan,Taha Bilel Chalbi,Ennouamane Saalaoui,Abdeslam Asehraou,Alexandre Noiriel,Abdelkarim Abousalham,Hocine Hacene,Amel Bouanane-Darenfed,Marilize Le Roes-Hill,Bassem Jaouadi

Journal

International Journal of Biological Macromolecules

Published Date

2022/12/1

Heterologous expression, kinetic characterization and molecular modeling of a new sn-1, 3-regioselective triacylglycerol lipase from Serratia sp. W3

A recombinant Serratia sp. W3 lipase (rSmL) was expressed in E. coli and purified to homogeneity. The kinetic properties, regio-selectivity, and interfacial performances of rSmL were compared with those of the native lipase (nSmL). rSmL has a molecular mass of 67 kDa and a specific activity of 3530 U mg−1 which is around 12-fold higher than that of nSmL (300 U mg−1) when using olive oil as the substrate. Both rSmL and nSmL were able to hydrolyze the ester bond at the sn-1 and sn-3 positions but exhibited a clear regio-preference towards the sn-3 position of the surface-coated triglycerides (TG), which were esterified with α-eleostearic acid at the sn-1/3 position or dicaprin isomers spread as monomolecular films. Molecular modeling and docking of TG into the active site of rSmL indicated that this regio-preference may be due to steric hindrance, created by the residues Ile308 and Trp311, with distances …

Authors

Ahlem Eddehech,Renaud Rahier,Nabil Smichi,Yani Arhab,Alexandre Noiriel,Abdelkarim Abousalham,Adel Sayari,Zied Zarai

Journal

Process Biochemistry

Published Date

2021/4/1

Phospholipase D inhibitors screening: Probing and evaluation of ancient and novel molecules

Phospholipase D (PLD) is a ubiquitous enzyme that cleaves the distal phosphoester bond of phospholipids generating phosphatidic acid (PA). In plants, PA is involved in numerous cell responses triggered by stress. Similarly, in mammals, PA is also a second messenger involved in tumorigenesis. PLD is nowadays considered as a therapeutic target and blocking its activity with specific inhibitors constitutes a promising strategy to treat cancers. Starting from already described PLD inhibitors, this study aims to investigate the effect of their structural modifications on the enzyme's activity, as well as identifying new potent inhibitors of eukaryotic PLDs. Being able to purify the plant PLD from Vigna unguiculata (VuPLD), we obtained a SAXS model of its structure. We then used a fluorescence-based test suitable for high-throughput screening to review the effect of eukaryotic PLD inhibitors described in the literature. In this …

Authors

Yani Arhab,Karim Bessaa,Houda Abla,Meryem Aydin,Renaud Rahier,Arnaud Comte,Leyre Brizuela,Saïda Mebarek,Florent Perret,Mickaël V Cherrier,Abdelkarim Abousalham,Alexandre Noiriel

Journal

International Journal of Biological Macromolecules

Published Date

2021/1/1

Professor FAQs

What is abdelkarim abousalham's h-index at Université Claude Bernard Lyon 1?

The h-index of abdelkarim abousalham has been 14 since 2020 and 25 in total.

What are abdelkarim abousalham's research interests?

The research interests of abdelkarim abousalham are: Biochimie, enzymologie, lipolyse enzymatique

What is abdelkarim abousalham's total number of citations?

abdelkarim abousalham has 2,076 citations in total.

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