AbdelHameed MS

AbdelHameed MS

Beni-Suef University

H-index: 14

Africa-Egypt

Professor Information

University

Beni-Suef University

Position

Professor ( Egypt and Jazan University KSA)

Citations(all)

718

Citations(since 2020)

343

Cited By

504

hIndex(all)

14

hIndex(since 2020)

11

i10Index(all)

18

i10Index(since 2020)

12

Email

University Profile Page

Beni-Suef University

Research & Interests List

wastewater treatment

immobilized

antimicrobial activity

wadi el rayan

Top articles of AbdelHameed MS

Glycine differentially improved the growth and biochemical composition of Synechocystis sp. PAK13 and Chlorella variabilis DT025

The potential of microalgae to produce valuable compounds has garnered considerable attention. However, there are various challenges that hinder their large-scale industrial utilization, such as high production costs and the complexities associated with achieving optimal growth conditions. Therefore, we investigated the effects of glycine at different concentrations on the growth and bioactive compounds production of Synechocystis sp. PAK13 and Chlorella variabilis cultivated under nitrogen availability. Glycine supplementation resulted in increased biomass and bioactive primary metabolites accumulation in both species. Sugar production, particularly glucose content, significantly improved in Synechocystis at 3.33 mM glycine (1.4 mg/g). This led to enhanced organic acid, particularly malic acid, and amino acids production. Glycine stress also influenced the concentration of indole-3-acetic acid, which was significantly higher in both species compared to the control. Furthermore, fatty acids content increased by 2.5-fold in Synechocystis and by 1.36-fold in Chlorella. Overall, the exogenous application of glycine is a cheap, safe, and effective approach to enhancing sustainable microalgal biomass and bioproducts production.

Authors

Wael A Fathy,Hamada AbdElgawad,Ehab A Essawy,Eman Tawfik,Mohamed S Abdelhameed,Ola Hammouda,Shereen Magdy Korany,Khaled NM Elsayed

Journal

Frontiers in Bioengineering and Biotechnology

Published Date

2023/6/1

Seasonal changes in the biochemical composition of dominant macroalgal species along the Egyptian Red Sea Shore

Simple Summary Macroalgae play a significant role as primary producers in marine ecosystems. The most dominant species at the three collection sites studied along the Egyptian Red Sea were seasonally harvested. Among several species collected, five dominant macroalgae (Caulerpa prolifera, Acanthophora spicifera, Cystoseira myrica, Cystoseira trinodis and Turbinaria ornata) were selected for further studies. These macroalgae were identified using morphological and molecular characteristics. During summer and winter, the mineral content and biochemical composition of the selected macroalgal species were evaluated. These analyses indicated that macroalgae are rich in minerals as well as primary and secondary metabolites. Moreover, the findings reported that the macroalgae studied possess high nutritional value in the summer more than in the winter season. Abstract Macroalgae are significant biological resources in coastal marine ecosystems. Seasonality influences macroalgae biochemical characteristics, which consequentially affect their ecological and economic values. Here, macroalgae were surveyed from summer 2017 to spring 2018 at three sites at 7 km (south) from El Qusier, 52 km (north) from Marsa Alam and 70 km (south) from Safaga along the Red Sea coast, Egypt. Across all the macroalgae collected, Caulerpa prolifera (green macroalgae), Acanthophora spicifera (red macroalgae) and Cystoseira myrica, Cystoseira trinodis and Turbinaria ornata (brown macroalgae) were the most dominant macroalgal species. These macroalgae were identified at morphological and molecular (18s …

Authors

Marwa Kamal,Neveen Abdel-Raouf,Khairiah Alwutayd,Hamada AbdElgawad,Mohamed Sayed Abdelhameed,Ola Hammouda,Khaled NM Elsayed

Journal

Biology

Published Date

2023/3/7

Applying an internal transcribed spacer as a single molecular marker to differentiate between Tetraselmis and Chlorella species

In the realm of applied phycology, algal physiology, and biochemistry publications, the absence of proper identification and documentation of microalgae is a common concern. This poses a significant challenge for non-specialists who struggle to identify numerous eukaryotic microalgae. However, a promising solution lies in employing an appropriate DNA barcoding technique and establishing comprehensive databases of reference sequences. To address this issue, we conducted a study focusing on the molecular characterization and strain identification of Tetraselmis and Chlorella species, utilizing the internal transcribed spacer (ITS) barcode approach. By analyzing the full nuclear ITS region through the Sanger sequencing approach, we obtained ITS barcodes that were subsequently compared with other ITS sequences of various Tetraselmis and Chlorella species. To ensure the reliability of our identification procedure, we conducted a meticulous comparison of the DNA alignment, constructed a phylogenetic tree, and determined the percentage of identical nucleotides. The findings of our study reveal the significant value of the ITS genomic region as a tool for distinguishing and identifying morphologically similar chlorophyta. Moreover, our results demonstrate that both the ITS1 and ITS2 regions are capable of effectively discriminating isolates from one another; however, ITS2 is preferred due to its greater intraspecific variation. These results underscore the indispensability of employing ITS barcoding in microalgae identification, highlighting the limitations of relying solely on morphological characterization.

Authors

Wael A Fathy,Natascha Techen,Khaled NM Elsayed,Ehab A Essawy,Eman Tawfik,Khairiah Mubarak Alwutayd,Mohamed S Abdelhameed,Ola Hammouda,Samir A Ross

Journal

Frontiers in Microbiology

Published Date

2023/8/23

Antibacterial, antifungal, and anticancer effects of camel milk exosomes: an in vitro study

Simple Summary Camel milk (CM) and its exosomes (CM-EXO) have many health-promoting effects due to their antibacterial, antifungal, and anticancer effects. Herein, we investigated the CM-EXO antimicrobial effect on Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus, and Enterococcus feacalis), Gram-negative strains (Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis), and Candida albicans and found only bacteriostatic effects against Gram-negative strains, and fungistatic effect. To the best of our knowledge, this is the first study to report a selective apoptotic effect of CM-EXOs on HepG2 and CaCo2 cells, but not on normal Vero cells. CM-EXOs also induced the elevation of intracellular reactive oxygen species and reduced antioxidant status in cancer cells but not in normal cells. Abstract Camel milk (CM) has potent antibacterial and antifungal effects and camel milk exosomes (CM-EXO) have been shown to inhibit the proliferation of a large variety of cancer cells including HepaRG, MCF7, Hl60, and PANC1. However, little is known regarding the effects of CM-EXO on bacteria, fungi, HepG2, CaCo2, and Vero cells. Therefore, this study aimed to evaluate the antibacterial, antifungal, and anticancer effects of CM-EXO. EXOs were isolated from CM by ultracentrifugation and characterized by transmission electron microscope and flow cytometry. Unlike CM, CM-EXO (6 mg/mL) had no bactericidal effects on Gram-positive bacteria (Staphylococcus aureus, Micrococcus luteus, and Enterococcus feacalis) but they had bacteriostatic effects, especially against Gram-negative strains …

Authors

Amira M Shaban,Mai Raslan,Zeina Walid Sharawi,Mohamed Sayed Abdelhameed,Ola Hammouda,Hossam M El-Masry,Khaled NM Elsayed,Mohammed A El-Magd

Journal

Veterinary Sciences

Published Date

2023/2/6

Exploring Exogenous Indole-3-acetic Acid’s Effect on the Growth and Biochemical Profiles of Synechocystis sp. PAK13 and Chlorella variabilis

Microalgae have garnered scientific interest for their potential to produce bioactive compounds. However, the large-scale industrial utilization of microalgae faces challenges related to production costs and achieving optimal growth conditions. Thus, this study aimed to investigate the potential role of exogenous indole-3-acetic acid (IAA) application in improving the growth and production of bioactive metabolites in microalgae. To this end, the study employed different concentrations of exogenously administered IAA ranging from 0.36 µM to 5.69 µM to assess its influence on the growth and biochemical composition of Synechocystis and Chlorella. IAA exposure significantly increased IAA levels in both strains. Consequentially, improved biomass accumulation in parallel with increased total pigment content by approximately eleven-fold in both strains was observed. Furthermore, the application of IAA stimulated the accumulation of primary metabolites. Sugar levels were augmented, providing a carbon source that facilitated amino acid and fatty acid biosynthesis. As a result, amino acid levels were enhanced as well, leading to a 1.55-fold increase in total amino acid content in Synechocystis and a 1.42-fold increase in Chlorella. Total fatty acids content increased by 1.92-fold in Synechocystis and by 2.16-fold in Chlorella. Overall, the study demonstrated the effectiveness of exogenously adding IAA as a strategy for enhancing the accumulation of microalgae biomass and biomolecules. These findings contribute to the advancement of microalgae-based technologies, opening new avenues to produce economically important compounds derived from …

Authors

Wael A Fathy,Hamada AbdElgawad,Amr H Hashem,Ehab Essawy,Eman Tawfik,Abdulaziz A Al-Askar,Mohamed S Abdelhameed,Ola Hammouda,Khaled NM Elsayed

Journal

Molecules

Published Date

2023/7/19

Insights into random mutagenesis techniques to enhance biomolecule production in microalgae: Implications for economically viable bioprocesses

Microalgae, as a diverse photosynthetic group of microorganisms with substantial ecological significance, have enormous potential for use in the food, medicine, and energy industries. However, current limitations in cost-effective production restrict their ubiquitous use. The purpose of this comprehensive mini-review is to investigate the impact of random mutagenesis techniques on microalgae and their biomolecule content, with the intention of improving the productivity and sustainability of cell factories for economically viable bioprocesses. Moreover, due to their simplicity and ease of cultivation in laboratory settings, microalgae serve as valuable model organisms for numerous scientific studies. This study investigates the effects of chemical and physical mutagens on microalgae in order to generate a heterogeneous microalgal population with advantageous traits such as increased lipids, proteins, and carotenoids, accelerated development, and enhanced nutrient absorption. This study findings will provide valuable insights into the manipulation of microalgae to increase biomolecule production and release their potential for a variety of applications.

Authors

Wael A Fathy,Natascha Techen,Khaled Elsayed,Ehab Essawy,Eman Tawfik,Mohamed Sayed Abdelhameed,Ola Hammouda,Samir A Ross

Journal

International Aquatic Research

Published Date

2023/6/1

Overexpressing key enzymes in lipogenesis to boost microalgae cellular oil content for biofuel production: a mini-review

Rapid population growth globally and urbanization have caused a significant drop in fossil fuel feedstocks, pushing countries to seek alternative sources. Microalgae are a feasible biofuel feedstock due to their high photosynthetic efficiency, which gives various potential benefits for the environmentally friendly biofuel production process, besides the high biomass productivity strains required for a long-term integrated platform. Consequently, modifying lipid metabolic pathways to increase lipid production in microalgae cells consider a viable strategy. Overexpression and transformation of key genes involved in lipid biosynthesis are regarded to be crucial methods for increasing lipid production. Whereas transformation and gene editing are two approaches affecting these alterations, that have been sufficiently established in microalgae, transforming these strains into a very adaptive stage for successfully designing ways to produce biofuel. The current mini-review focuses on potential strategies for improving microalgae metabolic engineering, specifically lipogenesis, by regulated overexpression of native genes or transgenes such as acetyl coenzyme-A carboxylase, diacylglycerol acyltransferase, and malic enzyme genes, as well as current limitations and gaps and future directions.

Authors

Wael A Fathy,Ehab Essawy,Eman Tawfik,Rania Omar,Mohamed S Abdelhameed,Ola Hammouda,Neveen Abdel-Raouf,Khaled NM Elsayed

Published Date

2022/6/1

Flocculation of microalgae using calcium oxide nanoparticles; process optimization and characterization.

The massive energy required for biomass gathering makes current commercial microalgal biofuel manufacturing economically unsustainable. A present harvesting method is provided as an inexpensive and energy-efficient way; nano-flocculation by employing nanoparticles to concentrate and flocculate microalgae of interest. In this study, we used calcium oxide nanoparticles (CaONPs) derived from waste products of the Fayoum Sugar Factory (FSF) as a low-cost flocculent for a microalgae flocculation process that was tuned using three control parameters: temperature, flocculent dose, and medium pH values. Furthermore, FSF wastewater was employed as a nourishment source for microalgae production. FT-IR, X-RD, Zeta potential, and TEM were used to further identify and characterize CaONPs. According to the current data, using CaONPs under the best conditions for algae harvesting (pH 9.8 and temperature …

Authors

Mamdouh HH Mohamed,Ayman H Zaki,Neveen Abdel-Raouf,Khawla I Alsamhary,Wael A Fathy,Mohamed S Abdelhameed,Khaled NM Elsayed

Journal

International Aquatic Research

Published Date

2022/3/1

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