Abdelhalim Loukil, Ph.D.

Abdelhalim Loukil, Ph.D.

Duke University

H-index: 7

North America-United States

Description

Abdelhalim Loukil, Ph.D., With an exceptional h-index of 7 and a recent h-index of 7 (since 2020), a distinguished researcher at Duke University, specializes in the field of Cilia and Human Disease.

Professor Information

University

Duke University

Position

___

Citations(all)

209

Citations(since 2020)

160

Cited By

109

hIndex(all)

7

hIndex(since 2020)

7

i10Index(all)

6

i10Index(since 2020)

5

Email

University Profile Page

Duke University

Research & Interests List

Cilia and Human Disease

Top articles of Abdelhalim Loukil, Ph.D.

Identification of new ciliary signaling pathways in the brain and insights into neurological disorders

Primary cilia are conserved sensory hubs essential for signaling transduction and embryonic development. Ciliary dysfunction causes a variety of developmental syndromes with neurological features and cognitive impairment, whose basis mostly remains unknown. Despite connections to neural function, the primary cilium remains an overlooked organelle in the brain. Most neurons have a primary cilium; however, it is still unclear how this organelle modulates brain architecture and function, given the lack of any systemic dissection of neuronal ciliary signaling. Here, we present the first in vivo glance at the molecular composition of cilia in the mouse brain. We have adapted in vivo BioID (iBioID), targeting the biotin ligase BioID2 to primary cilia in neurons. We identified tissue-specific signaling networks enriched in neuronal cilia, including Eph/Ephrin and GABA receptor signaling pathways. Our iBioID ciliary network presents a wealth of neural ciliary hits that provides new insights into neurological disorders. Our findings are a promising first step in defining the fundamentals of ciliary signaling and their roles in shaping neural circuits and behavior. This work can be extended to pathological conditions of the brain, aiming to identify the molecular pathways disrupted in the brain cilium. Hence, finding novel therapeutic strategies will help uncover and leverage the therapeutic potential of the neuronal cilium.

Authors

Abdelhalim Loukil,Emma Ebright,Akiyoshi Uezu,Yudong Gao,Scott H Soderling,Sarah C Goetz

Journal

bioRxiv

Published Date

2023

Multiple ciliary localization signals control INPP5E ciliary targeting

Primary cilia are solitary membrane protrusions acting as cellular antennae. They emanate from the basal body, a specialized mother centriole, and consist of a microtubule shaft, or axoneme, surrounded by the ciliary membrane, which is topologically continuous with, but compositionally distinct from, the plasma membrane (PM). For cilia to perform their signaling functions, they must accumulate specific receptors and signal transducers. For this to happen, these proteins must first reach the ciliary base, from where they can enter cilia by crossing the transition zone (TZ), the border region separating the ciliary compartment from the rest of the cell. If they can make it inside cilia, the ciliary levels of these proteins will then depend on the balance between ciliary entry and exit rates, a balance that can shift over time. Ciliary entry and exit rates in turn depend on how proteins interact with TZ components, and on whether they associate with specialized ciliary trafficking machinery, such as intraflagellar transport (IFT) trains, microtubule motor-driven multiprotein assemblies whose components, like the IFT-B, IFT-A, and BBSome complexes, selectively bind ciliary cargoes to mediate their transport into or out of cilia (Nachury and Mick, 2019; Reiter and Leroux, 2017; Garcia-Gonzalo and Reiter, 2017).Ciliary malfunction causes ciliopathies, a diverse group of human diseases, many of which are rare autosomal recessive syndromes. One such disease is Joubert syndrome (JBTS), affecting≈ 1 in 100,000 people worldwide and whose pathognomonic signature is the molar tooth sign (MTS), a cerebellar and midbrain malformation observable by magnetic …

Authors

Dario Cilleros-Rodriguez,Raquel Martin-Morales,Pablo Barbeito,Abhijit Deb Roy,Abdelhalim Loukil,Belen Sierra-Rodero,Gonzalo Herranz,Olatz Pampliega,Modesto Redrejo-Rodriguez,Sarah C Goetz,Manuel Izquierdo,Takanari Inoue,Francesc R Garcia-Gonzalo

Journal

Elife

Published Date

2022/9/5

A complex of distal appendage–associated kinases linked to human disease regulates ciliary trafficking and stability

Cilia biogenesis is a complex, multistep process involving the coordination of multiple cellular trafficking pathways. Despite the importance of ciliogenesis in mediating the cellular response to cues from the microenvironment, we have only a limited understanding of the regulation of cilium assembly. We previously identified Tau tubulin kinase 2 (TTBK2) as a key regulator of ciliogenesis. Here, using CRISPR kinome and biotin identification screening, we identify the CK2 catalytic subunit CSNK2A1 as an important modulator of TTBK2 function in cilia trafficking. Superresolution microscopy reveals that CSNK2A1 is a centrosomal protein concentrated at the mother centriole and associated with the distal appendages. Csnk2a1 mutant cilia are longer than those of control cells, showing instability at the tip associated with ciliary actin cytoskeleton changes. These cilia also abnormally accumulate key cilia assembly and …

Authors

Abdelhalim Loukil,Chloe Barrington,Sarah C Goetz

Journal

Proceedings of the National Academy of Sciences

Published Date

2021/4/20

Professor FAQs

What is Abdelhalim Loukil, Ph.D.'s h-index at Duke University?

The h-index of Abdelhalim Loukil, Ph.D. has been 7 since 2020 and 7 in total.

What are Abdelhalim Loukil, Ph.D.'s research interests?

The research interests of Abdelhalim Loukil, Ph.D. are: Cilia and Human Disease

What is Abdelhalim Loukil, Ph.D.'s total number of citations?

Abdelhalim Loukil, Ph.D. has 209 citations in total.

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