Abdelhadi Abdallah Abdelhadi

Abdelhadi Abdallah Abdelhadi

Cairo University

H-index: 12

Africa-Egypt

Professor Information

University

Cairo University

Position

Professor Dept. of Genetics Faculty of Agriculture

Citations(all)

487

Citations(since 2020)

363

Cited By

215

hIndex(all)

12

hIndex(since 2020)

10

i10Index(all)

16

i10Index(since 2020)

10

Email

University Profile Page

Cairo University

Research & Interests List

Molecular Genetics

Top articles of Abdelhadi Abdallah Abdelhadi

Cereal crop genome editing tools and their applications to sustainable agriculture

Sustainable agriculture is essential to attain food security and address increasing climate change concerns. Population, abiotic and biotic stresses and limited agricultural water supplies and land are important barriers to cereal crops production, in addition to extreme weather. These factors influence their quality and productivity. Therefore, there is a pressing need for effective methods of food production under these conditions. For many individuals in low-and middle-income nations, cereals represent a key food source. Developing cereal crops with enhanced flexibility, high yields, and the ability to tolerate these biotic and abiotic problems is therefore essential. Modern OMICS techniques, next-generation sequencing, bioinformatics tools and the most recent improvements in genome editing tools (GET) have made targeted mutagenesis conceivable. By altering a crop variety's endogenous genome, which is free of any foreign genes, genome editing (GE) enhances the crop variety. Therefore, crops that have undergone GE but have not integrated foreign genes are not considered genetically modified organisms (GMOs) in a number of countries. GE is being used by researchers to promote the nutritional value of cereal crops and increase their tolerance to biotic and abiotic stresses. This review critically discusses the activities of GET, the role of bioinformatics tools and its application to sustainable agriculture for cereal crops.

Authors

Nagwa I ELARABI,Abd El-Latif HESHAM,Hossam S EL-BELTAGI,Tarek A SHALABY,Mohamed S AL-SAIKHAN,Amal A MOHAMED,Abdelhadi A Abdelhadi

Journal

Notulae Botanicae Horti Agrobotanici Cluj-Napoca

Published Date

2024/2/13

Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium

Lead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated by heavily lead-containing industries. Eight lead-resistant strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as a particularly promising candidate, demonstrating an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight. Furthermore, E. kobei FACU6 displayed a remarkable a maximum tolerance concentration (MTC) for lead reaching 3,000 mg/L. To further investigate the morphological changes in E. kobei FACU6 in response to lead exposure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. These analyses revealed significant lead adsorption and intracellular accumulation in treated bacteria in contrast to the control bacterium. Whole-genome sequencing was performed to gain deeper insights into E. kobei’s lead resistance mechanisms. Structural annotation revealed a genome size of 4,856,454 bp, with a G + C content of 55.06%. The genome encodes 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. Notably, genes associated with heavy metal resistance and their corresponding regulatory elements were identified within the genome. Furthermore, the expression levels of four specific heavy metal resistance …

Authors

Hossam S El-Beltagi,Asmaa A Halema,Zainab M Almutairi,Hayfa Habes Almutairi,Nagwa I Elarabi,Abdelhadi A Abdelhadi,Ahmed R Henawy,Heba AR Abdelhaleem

Journal

Frontiers in Bioengineering and Biotechnology

Published Date

2024/1/8

Exopolysaccharide production from agro-industrial wastes by lactic acid bacteria isolated from silage

Materials and methodsLAB-producing EPS isolates were molecularly identified by the 16S rRNA gene sequencing and deposited their DNA sequences to NCBI. EPS production using the examined 13 strains was carried out on MRS as a standard production medium and ranged between 1.53 and 7.53 g/l. Then, the highest significant EPS-producing strains ie, Lacticaseibacillus paracasei strain LAB 64, Lacticaseibacillus rhamnosus strain LAB 160, and Lacticaseibacillus rhamnosus strain LAB 192 were further examined for EPS production from the agro-industrial wastes sugarcane molasses, salted cheese whey, and their mixture.

Authors

Ahmed R Henawy,Abdelhadi A Abdelhadi,Asmaa A Halema,Refae I Refae,Olfat S Barakat

Journal

Egyptian Pharmaceutical Journal

Published Date

2023/7/1

Anaerobic Biotechnology: Implementations and New Advances

Anaerobic technology has gained widespread acceptance in environmental sustainability as a low-cost alternative for pollution control. The anaerobic technologies for contaminants treatment have three essential returns, i.e., bioenergy recovery, energy-saving and low sludge production. Therefore, the anaerobic process will be the favored green treatment technology for a sustainable environment in years to come. Currently, anaerobic treatment remains to flourish in several features, such as reactors development, bio-hythane production, molecular techniques for microbial studies and kinetic modeling and extending applications to a wide range of waste and wastewater effluents. Therefore, this chapter brings together the most up-to-date information on the new developments in anaerobic technology. Also, it sheds light on the current conversion methods and technologies for energy recovery with a focus on the use …

Authors

Samir I Gadow,Hatem Hussein,Abdelhadi A Abdelhadi,Abd El-Latif Hesham

Published Date

2023/4/5

Draft genome of Raoultella planticola, a high lead resistance bacterium from industrial wastewater

Isolation of heavy metals-resistant bacteria from their original habitat is a crucial step in bioremediation. Six lead (Pb) resistant bacterial strains were isolated and identified utilizing 16S rRNA to be Enterobacter ludwigii FACU 4, Shigella flexneri FACU, Microbacterium paraoxydans FACU, Klebsiella pneumoniae subsp. pneumonia FACU, Raoultella planticola FACU 3 and Staphylococcus xylosus FACU. It was determined that all these strains had their Minimum inhibitory concentration (MIC) to be 2500 ppm except R. planticola FACU 3 has a higher maximum tolerance concentration (MTC) up to 2700 ppm. We evaluated the survival of all six strains on lead stress, the efficiency of biosorption and lead uptake. It was found that R. planticola FACU 3 is the highest MTC and S. xylosus FACU was the lowest MTC in this evaluation. Therefore, transmission electron microscopy (TEM) confirmed the difference between the …

Authors

Nagwa I Elarabi,Asmaa A Halema,Abdelhadi A Abdelhadi,Ahmed R Henawy,Omar Samir,Heba AR Abdelhaleem

Journal

AMB Express

Published Date

2023/1/30

Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase gene

The phenylurea herbicides are persistent in soil and water, necessitating the creation of methods for removing them from the environment. This study aimed to examine the soil microbial diversity, searching for local bacterial isolates able to efficiently degrade the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1, 1-dimethylurea (IPU). The best isolates able to effectively degrade IPU were selected, characterized, and identified as Pseudomonas putida and Acinetobacter johnsonii. The catechol 1, 2-dioxygenase enzyme's catA gene was amplified, cloned, and expressed in E. coli M15. The Expressed E. coli showed high degradation efficiency (44.80%) as analyzed by HPLC after 15 days of inoculation in comparison to P. putida (21.60%). The expression of the catA gene in P. putida and expressed E. coli was measured using quantitative polymerase chain reaction (qPCR). The results displayed a significant …

Authors

Nagwa I Elarabi,Abdelhadi A Abdelhadi,Amr A Nassrallah,Mahmoud SM Mohamed,Heba AR Abdelhaleem

Journal

AMB Express

Published Date

2023/9/26

Biochar and compost-based integrated nutrient management: potential for carbon and microbial enrichment in degraded acidic and charland soils

Soil acidification and charland formation through alluvial sand depositions are emerging threats to food security in Bangladesh, in that they endanger crop production in about 35% of its territory. The integrated plant nutrient system (IPNS) is a globally accepted nutrient management approach designed to revive the damaged soils’ fertility level. Total organic carbon (TOC) in soil is a composite index of soil quality that has consequences for agricultural productivity and natural soil ecosystems. The present research was done to assess the impacts using biochar, compost, poultry litter, and vermicompost-based IPNS approaches on labile and TOC pools, TOC stocks, lability and management indices, and microbial population under different cropping patterns after two years in acidic and charland soils. The application of IPNS treatments raised microbial biomass carbon (MBC) by 9.1-50.0% in acidic soil and 8.8-41.2% in charland soil compared to the untreated soil, with the largest increase in poultry manure biochar (PMB). Microbial biomass nitrogen (MBN) increased from 20% to 180% in charland soil compared to the control although no effect was observed in acidic soil. Basal respiration (BR) rose by 43% to 429% in acidic soil and 16% to 189% in charland soil compared to the control, exhibiting the highest value in PMB. IPNS treatments significantly improved SOC and POC but did not affect POXc and bulk density in both soils. PMB and organic fertilizer (OF, compost)-based IPNS wielded the greatest influence on lability index of MBC in acidic soil and management index of MBC in both soils. This is despite the fact that IPNS did not affect the …

Authors

MM Rahman,Md Rafiqul Islam,Shihab Uddin,Mohammad Mahmudur Rahman,Ahmed Gaber,Abdelhadi A Abdelhadi,MMR Jahangir

Journal

Frontiers in Environmental Science

Published Date

2022/1/7

Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

Genome editing tools have rapidly been adopted by plant scientists for crop improvement. Genome editing using a multiplex sgRNA-CRISPR/Cas9 genome editing system is a useful technique for crop improvement in monocot species. In this study, we utilized precise gene editing techniques to generate wheat 3’(2’), 5’-bisphosphate nucleotidase (TaSal1) mutants using a multiplex sgRNA-CRISPR/Cas9 genome editing system. Five active TaSal1 homologous genes were found in the genome of Giza168 in addition to another apparently inactive gene on chromosome 4A. Three gRNAs were designed and used to target exons 4, 5 and 7 of the five wheat TaSal1 genes. Among the 120 Giza168 transgenic plants, 41 lines exhibited mutations and produced heritable TaSal1 mutations in the M1 progeny and 5 lines were full 5 gene knock-outs. These mutant plants exhibit a rolled-leaf phenotype in young leaves and …

Authors

Abdelhadi A. Abdelhadi & Nagwa I. Elarabi Naglaa A. Abdallah,Hany Elsharawy,Hamiss A. Abulela,Roger Thilmony

Journal

gm crops & food

Published Date

2022/10/6

Professor FAQs

What is Abdelhadi Abdallah Abdelhadi's h-index at Cairo University?

The h-index of Abdelhadi Abdallah Abdelhadi has been 10 since 2020 and 12 in total.

What are Abdelhadi Abdallah Abdelhadi's research interests?

The research interests of Abdelhadi Abdallah Abdelhadi are: Molecular Genetics

What is Abdelhadi Abdallah Abdelhadi's total number of citations?

Abdelhadi Abdallah Abdelhadi has 487 citations in total.

academic-engine

Useful Links