abdelfattah badr

abdelfattah badr

Helwan University

H-index: 30

Africa-Egypt

abdelfattah badr Information

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Helwan University

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Citations(all)

3612

Citations(since 2020)

1291

Cited By

2676

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30

hIndex(since 2020)

18

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62

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34

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Helwan University

abdelfattah badr Skills & Research Interests

plant science

Top articles of abdelfattah badr

Genome editing by site-directed nucleases and its applications in producing climate change resilient crop plants

Climate change is long-term variations in weather patterns such as temperature, rainfall, and soil salinity. Several parts of the world are expected to suffer extreme environmental conditions with the expected impacts of climate change. Genome-editing techniques offer potential applications to plants’ adaptations to the environmental extremes imposed by climate changes. Three DNA cleavage enzymes have been used for genome change in the last two decades including the zinc-finger nucleases and the transcription activator-like effector nucleases. However, in the last decade, the clustered regularly interspaced short palindromic repeats (CRISPR-Cas) is the most widely used genome-editing system because of their adaptability and ease of operation. The CRISPR-Cas approach has been increasingly modified, and new techniques based on this approach have been developed based on enzymes that recognize …

Authors

Abdelfattah Badr,Hanaa H El-Shazly

Published Date

2024

ISSR Markers Related to Effects of Gamma Irradiation on Growth and Yield Parameters of Fenugreek (Trigonella foenum-graecum L.)

The impact of gamma irradiation on fenugreek (Trigonella foenum-graecum L.) for two generations was investigated in this research. The study involved exposing dry seeds of fenugreek to five doses of gamma rays (25, 50, 75, 100, and 200 Gy) using a cobalt 60 gamma radiation source. The irradiated and unirradiated seeds were grown in the field for two consecutive seasons. The results showed significant changes in vegetative growth traits as well as yield parameters in both the M1 and M2 generations. Low doses of 25–100 Gy stimulated growth and increased yield in M1 and M2 plants, while the highest dose of 200 Gy showed an inhibitory effect on the same parameters. The 75 Gy resulted in the highest increases in all traits. Eleven ISSR primers were used to identify molecular differences in ISSR fingerprinting in response to γ-irradiation treatments in the M2 plants. The 11 ISSR primers produced 118 markers, revealed as bands on the agarose gel, including 73 monomorphic bands, 26 polymorphic bands, and 19 unique bands with an average polymorphism of 37%. The cluster analysis of ISSR data differentiated the M2 plants grown from seeds exposed to 75 Gy of gamma radiation, indicating that the highest increase in vegetative and yield traits was associated with polymorphic ISSR markers. This result shows that ISSR profiling is a good way to connect genetic differences that happen in plants of the M2 generation in response to γ-irradiation treatments.

Authors

Nora S El-Gazzar,Laila Mekki,Akram A Aboseidah,El-Shahat El-Mursi,Abdelfattah Badr

Journal

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Published Date

2023/12/14

Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings

BackgroundSeaweeds are a viable bioresource for suffering plants against salt stress, as they abundant in nutrients, hormones, vitamins, secondary metabolites, and many other phytochemicals that sustain plants’ growth under both typical and stressful situations. The alleviating capacity of extracts from three brown algae (Sargassum vulgare, Colpomenia sinuosa, and Pandia pavonica) in pea (Pisum sativum L.) was investigated in this study.MethodsPea seeds were primed for 2 h either with seaweed extracts (SWEs) or distilled water. Seeds were then subjected to salinity levels of 0.0, 50, 100, and 150 mM NaCl. On the 21st day, seedlings were harvested for growth, physiological and molecular investigations.ResultsSWEs helped reduce the adverse effects of salinity on pea, with S. vulgare extract being the most effective. Furthermore, SWEs diminished the effect of NaCl-salinity on germination, growth rate, and …

Authors

Marwa M Hamouda,Abdelfattah Badr,Sameh S Ali,Alia M Adham,Hanan I Sayed Ahmed,Khalil M Saad-Allah

Journal

Journal of Genetic Engineering and Biotechnology

Published Date

2023/12/1

Genetic diversity of Citrullus colocynthis populations using phytochemical analysis and SCoT marker variations

Citrullus colocynthis L. Schard (bitter melon) is a drought-resistant medicinal plant growing in Egypt and many other countries in the arid environments of the World. In Egypt, it is abundant in several locations in Egypt's Eastern Desert, extending from the Nile River eastward to the Red Sea, particularly in the Kosseir region on the Red Sea coast. It has a wide range of applications in traditional medicine due to its anti-inflammatory, antibacterial, antioxidant, and anesthetic features. In this study, the genetic diversity was explored using chemical analysis of the secondary metabolites in seed extract in 15 populations from different sites in the Eastern Desert of Egypt to correlate the chemical variation with genetic differences among populations as revealed by DNA fingerprinting using the Start Codon Targeted (SCoT) markers. A total of 81 chemical compounds were identified from the 15 populations. Retention time, peak …

Authors

Abdelfattah Badr,Hoida Zaki

Journal

Genetic Resources and Crop Evolution

Published Date

2023/11/7

Gamma-irradiation Induces Phenotypic and Genotypic Mutations in M3 and M4 Generations of Phaseolus vulgaris

Gamma irradiation might be an alternative strategy for increasing crop yield and/or introducing new varieties. Reddish-brown Phaseolus vulgaris L. cv Abo Yousuf was exposed to 50 Gy of γ-radiation produced M2 seeds, while 100 and 300 Gy were lethal. M2 seeds were cultivated to investigate the segregated traits in M3 and M4 plants. Segregation of purple and green: hypocotyls, lower venation, and pods, besides purple flowers and emarginated leaf apex, were observed in M3 plants. M3 seeds showed five seed coat colors: reddish-brown, brown, dark brown, grayish brown, and gray. The reddish-brown seeds had the highest weight, 30.74%, whereas the gray seeds had a much lighter weight, 23.14%. The flower color was light purple in three lines viz; control, M2 50Gy, and M3 reddish brown and white in M3 dark brown line. While M3 brown and gray seeds produced four flower colors: dark purple, purple, light purple, and white. Whereas, M3 grayish brown seeds produced three flower colors: dark purple, purple, and white. Furthermore, γ-radiation induced 15 new seed coat colors in M4 progeny. Molecular characterization showed different fingerprints correlated with the segregated morphological traits and seed coat colors. Both the PCR fingerprints and the dendrograms produced by cluster analysis of the selected four RAPD differentiated clearly between the different M3 segregating genotypes of cv Abo Yousuf from M2 and control plants more than the two ISSR primers. Such findings indicated the pleiotropic effects of seed coat color genes and provided helpful information on gene(s) that govern these traits.

Authors

Mohamed E El-Lithy,Shaimaa H Abdelgawad,Abdelfattah Badr

Journal

Egyptian Journal of Botany

Published Date

2023/9/1

Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

BackgroundWheat is a major cereal that can narrow the gap between the increasing human population and food production. In this connection, assessing genetic diversity and conserving wheat genetic resources for future exploitation is very important for breeding new cultivars that may withstand the expected climate change. The current study evaluates the genetic diversity in selected wheat cultivars using ISSR and SCoT markers, the rbcL and matK chloroplast DNA barcoding, and grain surface sculpture characteristics. We anticipate that these objectives may prioritize using the selected cultivars to improve wheat production. The selected collection of cultivars may lead to the identification of cultivars adapted to a broad spectrum of climatic environments.ResultsMultivariate clustering analyses of the ISSR and SCoT DNA fingerprinting polymorphism grouped three Egyptian cultivars with cultivar El-Nielain from …

Authors

Heba H Abouseada,Al-Safa H Mohamed,Samir S Teleb,Abdelfattah Badr,Mohamed E Tantawy,Shafik D Ibrahim,Faten Y Ellmouni,Mohamed Ibrahim

Journal

BMC Plant Biology

Published Date

2023/4/11

Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

BackgroundSilver nanoparticles (AgNPs) are the most widely used nanomaterial in agricultural and environmental applications. In this study, the impact of AgNPs solutions at 20 mg/L, 40 mg/L, 80 mg/L, and 160 mg/L on cell ultrastructure have been examined in pea (Pisum sativum L) using a transmission electron microscope (TEM). The effect of AgNPs treatments on the α, β esterase (EST), and peroxidase (POX) enzymes expression as well as gain or loss of inter-simple sequence repeats (ISSRs) markers has been described.ResultsDifferent structural malformations in the cell wall and mitochondria, as well as plasmolysis and vacuolation were recorded in root cells. Damaged chloroplast and mitochondria were frequently observed in leaves and the osmiophilic plastoglobuli were more observed as AgNPs concentration increased. Starch grains increased by the treatment with 20 mg/L AgNPs. The expressions of …

Authors

May Labeeb,Abdelfattah Badr,Soliman A Haroun,Magdy Z Mattar,Aziza S El-Kholy

Journal

Journal of Genetic Engineering and Biotechnology

Published Date

2022/12/1

Chromosomes as Sources of Taxonomic Information for Plant Systematics and Evolution

Chromosomes are the units of inheritance within the nuclei of all eukaryote cells and carry the genes as DNA packaged in the chromosomes forming the chromatin fibril with histone proteins. The DNA content of each chromosome is a single linear DNA double helix, which corresponds genetically to a linkage group. The chromosomes are only seen during cell division because of coiling up as chromatin in the non-dividing nucleus. In somatic cells, chromosomes occur as pairs of homologous chromosomes or homologs. During cell division, the chromosomes, condense into individual chromosomes during the stages of division (prophase, metaphase, anaphase, and telophase). The chromosomes are best studied at metaphase in somatic cells as each chromosome appears as two longitudinal chromatids. Each chromosome is composed of two arms separated by the centromere and terminated at the ends by telomeres. One member of each homologous chromosome is inherited from either the male or female plants, these homologs pair as bivalents in the first meiotic prophase. In about 45% of plants, more than two sets of chromosomes have been reported, these are known as polyploid numbers. The chromosome features that have been used as sources of taxonomic information include various attributes of chromosomes; the most common features are: 1) chromosome number, 2) chromosome size and gross morphology, 3) chromosomal variations in number and shape, 4) chromosome’s behavior at meiosis, 5) chromosome banding as revealed by differential staining of some chromosome parts and 6) in situ hybridization and chromosome …

Authors

Abdelfattah Badr

Journal

Taeckholmia

Published Date

2022/6/8

Molecular Phylogeny of Trifolium L. Section Trifolium with Reference to Chromosome Number and Subsections Delimitation

The genus Trifolium is one of the largest genera of the legume family Fabaceae with ca. 255 species. The genus is divided into eight sections; the section Trifolium is a major section of the genus, comprising 73 species mainly distributed in the Mediterranean region. We used nuclear ribosomal DNA internal transcribed spacer (ITS) and morphological variation to reconsider the delimitation and phylogenetic relationships of species in the section Trifolium with reference to chromosomal variations. Bayesian analysis of ITS data delimited the species as three clades based on the analysis of ITS sequence and informative indels in combination with morphological variation. The phylogeny of the species by different analyses methods does not support their current delimitation in 17 subsections. The basic chromosome number x = 8 is the number for the genus Trifolium, from which x = 7, 6 and 5 were derived through successive aneuploidy events. With reference to the distribution of these numbers in the species of the section Trifolium, species in clade III and clade II are more evolved than species in clade I.

Authors

H.I.S. Ahmed,A. Badr,H.H. El-Shazly,L. Watson,A.S. Fuoad,F.Y. Ellmouni

Journal

Plants

Published Date

2021

Expression of OsDREB2A in Transgenic Tomato Improves Drought Tolerance

Dehydration responsive element binding (DREB) are important regulatory molecules which have a crucial role in abiotic stress tolerance. The productivity of tomato, as a drought-sensitive crop, is highly restricted by drought stress. The current study aimed at introducing the OsDERB2A gene into two tomato genotypes via Agrobacterium-mediated transformation system. Cotyledonary explants were pre-cultured for two days with Agrobacterium strain LBA4404 harboring pCAMBIA1301 with OsDREB2A driven by the constitutive promoter CaMV35S for transformation. Shoots were directly regenerated on MS medium containing 1 mg l-1 zeatin and 1 mg l-1 BAP, and in presence of 30 mg l-1 hygromycin as selective agent. Only eight weeks were needed to regenerate transgenic tomato using this protocol. An OD600 of 0.4 resulted in 64.3-76.9% transformation efficiency. Stable integration and expression of the OsDREB2A gene were confirmed in transgenic tomato using PCR and RT-PCR analyses, and drought tolerance of T0 transgenic lines was confirmed by leaf disc assay in 300 mM mannitol. The superior biomass, photosynthetic pigments, free soluble sugars and proline accumulation of OsDREB2A transgenic lines over wild type in response to mannitol-stress revealed their enhanced drought tolerance and indicated that the constitutive expression of OsDREB2A might modulate the expression of other drought responsive genes.

Authors

Nada Hassan,Nadia M El-Shafey,Salah El-Din A Khodary,Hattem El-Shabrawi,Abdelfattah Badr

Published Date

2021

Plant responses to induced genotoxicity and oxidative stress by chemicals

In recent decades, the use of chemicals of anthropogenic activities such as agrochemicals, industrial and environmental chemicals, and nanoparticle has been growing with great benefits for food production and human health and welfare. However, hazards imposed by different categories of chemicals on plants and the whole ecosystem have been widely reported. Plants under stress suffer a rapid and transient overproduction of reactive oxygen species (ROS) that lead to DNA damage. Two of the major impacts of chemicals, not only in plants but also in all living organisms, are genotoxicity and oxidative stress. Oxidative stress may trigger some reactions that can be involved in stimulating genotoxicity in plants by inducing DNA damage that results in a variety of impairments to cell division and chromosomes. Genotoxicity assays have been developed in the last few decades to test permanent DNA-damage …

Authors

Abdelfattah Badr,Hanaa H El-Shazly,Heba I Mohamed

Journal

Induced genotoxicity and oxidative stress in plants

Published Date

2021

Studies on exogenous elicitors promotion of sulforaphane content in broccoli sprouts and its effect on the MDA-MB-231 breast cancer cell line

Broccoli (Brassica oleracea var. italica) belongs to the Brassicaceae family and is one of the most important cruciferous vegetables. It has gained popularity due to its high glucosinolate concentrations that have positive potential in cancer treatment. In this study, the effects of two elicitors; methyl jasmonate (MeJA) and salicylic acid (SA), on the production of sulforaphane from broccoli 7-days old seedlings, its antiapoptotic activity and its gene expression have been studied. Real-time PCR was used to quantify myrosinase (MY) gene expression associated with sulforaphane production. The antiapoptotic activity of sulforaphane treatments was evaluated and tested using MDA-MB-231 breast cancer cell line. The highest amount of sulforaphane was produced at 80 μM SA and 40 μM MeJA after 24 h of elicitation. Increased production of sulforaphane was found to be associated with over-expression of myrosinase gene …

Authors

May Ahmed Amer,Thoria Rashad Mohamed,Raoufa A Abdel Rahman,Manal Ali,Abdelfattah Badr

Journal

Annals of Agricultural Sciences

Published Date

2021/6/1

Efficacy of metal oxide nanoparticles as novel antimicrobial agents against multi-drug and multi-virulent Staphylococcus aureus isolates from retail raw chicken meat and giblets

Staphylococcus aureus is among the most common zoonotic pathogens originating from animals consumed as food, especially raw chicken meat (RCM). As far as we know, this might be the first report that explores the efficacy of metal oxide nanoparticles (MONPs), such as zinc peroxide nanoparticles (ZnO2-NPs), zinc oxide nanoparticles (ZnO-NPs), and titanium dioxide nanoparticles (TiO2-NPs) against multidrug resistant (MDR) and/or pandrug resistant (PDR) S. aureus strains with a strong biofilm-producing ability isolated from RCM and giblets. The overall prevalence of coagulase-positive staphylococci was 21%, with a contamination level range between 102 and 104 CFU/g. The incidence of virulence genes See (21/36), pvl (16/36), clfA (15/36), sec (12/36), tst (12/36), and sea (11/36) among S. aureus strains were relatively higher those of seb, sed, fnbA, and fnbB. For antimicrobial resistance gene …

Authors

Sameh S Ali,Mohamed S Moawad,Mohamed A Hussein,Maha Azab,Esraa A Abdelkarim,Abdelfattah Badr,Jianzhong Sun,Maha Khalil

Journal

International journal of food microbiology

Published Date

2021/4/16

Genetic diversity of a global collection of maize genetic resources in relation to their subspecies assignments, geographic origin, and drought tolerance

The genetic diversity among an international collection of 40 maize accessions has been evaluated using DNA ISSR fingerprinting. Among the 180 ISSR markers scored by 15 primers, 161 markers (89.59%) were polymorphic and 19 were unique in 16 accessions. A cluster tree based on the average distance coefficients and the Dice similarity indices divided the accessions into three major groups, each including clusters of accessions assigned to their subspecies. However, a low level of genetic differentiation among the accessions was demonstrated by the STRUCTURE analysis of ISSR data in agreement with the low gene flow (Nm) value among the accessions. A scatter diagram of the principal component analysis (PCA) based on ISSR data analysis revealed that the accessions were differentiated into three groups comparable to those produced by the cluster analysis, in which some accessions of the same subspecies showed a close similarity to each other. A scatter diagram of the principal coordinate analysis (PCoA) based on the drought tolerance indices (DTIs) showed that nine genetically similar accessions share drought tolerance characteristics; these include four of subsp. indurata, three of subsp. everata, and two of subsp. indentata. An abundance of unique ISSR alleles found in the 16 accessions, including the nine drought-tolerant accessions, represents rich untapped genetic resources and these accessions may be exploited in the future breeding of maize commercial lines.

Authors

Elham RS Soliman,Hanaa H El-Shazly,Andreas Börner,Abdelfattah Badr

Journal

Breeding science

Published Date

2021

Genetic diversity and volatile oil components variation in Achillea fragrantissima wild accessions and their regenerated genotypes

BackgroundWild medicinal plants are suffering natural environmental stresses and habitat destruction. The genetic diversity evaluation of wild accessions and their in vitro raised genotypes using molecular markers, as well as the estimation of substances of pharmaceutical value in wild plants and their regenerated genotypes are convenient approaches to test the genetic fidelity of regenerated plants as a source of substances of pharmaceutical value. In this study, the genetic diversity of 12 accessions of the medicinal plant Achillea fragrantissima, representing five sites in the mountains of South Sinai, Egypt, were estimated by the inter simple sequence repeats (ISSR) fingerprinting and their volatile oil components were identified using gas chromatography-mass spectrometry (GC-MS) analysis. The same accessions were regenerated in vitro and the genetic diversity and volatile oil components of propagated …

Authors

Abdelfattah Badr,Hanaa H El-Shazly,Mahmoud Sakr,Mai M Farid,Marwa Hamouda,Eman Elkhateeb,Hanan Syed Ahmad

Journal

Journal of Genetic Engineering and Biotechnology

Published Date

2021/12/1

Biodiversity of some Solanum species from southwestern Saudi Arabia’s highlands

The wild species of Solanum are important genetic resources for the medicinal and vegetable crop species of the genus and reservoir of genes for developing new economic uses of Solanum species. In this article, the genetic diversity and systematics of 14 Solanum accessions representing 11 species growing in the southwestern highlands of Saudi Arabia were evaluated using morphological variations and molecular polymorphism generated by 13 ISSR primers. Substantial variations were scored in 26 morphological characters and reflected in the cluster analysis of the examined accessions based on morphological variation. In the cluster trees illustrating the relatedness of the examined accessions based on morphological variation and ISSR polymorphism, the accessions of S. villosum and S. nigrum were differentiated as one cluster and S. dulcamara was associated with S. sisymbriifolium. In the meantime, S …

Authors

Gamal A El-Shaboury,Husain M Al-Wadi,Abdelfattah Badr

Journal

Botany Letters

Published Date

2021/4/3

Nanobiotechnological advancements in agriculture and food industry: Applications, nanotoxicity, and future perspectives

The high demand for sufficient and safe food, and continuous damage of environment by conventional agriculture are major challenges facing the globe. The necessity of smart alternatives and more sustainable practices in food production is crucial to confront the steady increase in human population and careless depletion of global resources. Nanotechnology implementation in agriculture offers smart delivery systems of nutrients, pesticides, and genetic materials for enhanced soil fertility and protection, along with improved traits for better stress tolerance. Additionally, nano-based sensors are the ideal approach towards precision farming for monitoring all factors that impact on agricultural productivity. Furthermore, nanotechnology can play a significant role in post-harvest food processing and packaging to reduce food contamination and wastage. In this review, nanotechnology applications in the agriculture and …

Authors

Sameh S Ali,Rania Al-Tohamy,Eleni Koutra,Mohamed S Moawad,Michael Kornaros,Ahmed M Mustafa,Yehia A-G Mahmoud,Abdelfattah Badr,Mohamed EH Osman,Tamer Elsamahy,Haixin Jiao,Jianzhong Sun

Published Date

2021/10/20

Molecular phylogeny of trifolium section trifolium with reference to chromosome number and subsections delimitation”. Plants, 2021, 10, 1985

The genus Trifolium is one of the largest genera of the legume family Fabaceae with ca. 255 species. The genus is divided into eight sections; the section Trifolium is a major section of the genus, comprising 73 species mainly distributed in the Mediterranean region. We used nuclear ribosomal DNA internal transcribed spacer (ITS) and morphological variation to reconsider the delimitation and phylogenetic relationships of species in the section Trifolium with reference to chromosomal variations. Bayesian analysis of ITS data delimited the species as three clades based on the analysis of ITS sequence and informative indels in combination with morphological variation. The phylogeny of the species by different analyses methods does not support their current delimitation in 17 subsections. The basic chromosome number x= 8 is the number for the genus Trifolium, from which x= 7, 6 and 5 were derived through successive aneuploidy events. With reference to the distribution of these numbers in the species of the section Trifolium, species in clade III and clade II are more evolved than species in clade I.

Authors

HIS Ahmed,A Badr,HH El-Shazly,L Watson,AS Fouad,FY Ellmouni

Published Date

2021

Umweltfreundliche Synthese von Silber-Nanopartikeln und ihre Auswirkungen auf das frühe Wachstum und die Zellteilung in den Wurzeln der Grünen Erbse (Pisum sativum L.)

The present study deals with the eco-synthesis and effects of silver nanoparticles (AgNPs) on pea (Pisum sativum L.). AgNPs were synthesized by using gelatine/glucose mixture as a reducing/stabilizing agent for silver nitrate. The AgNPs were characterized and their effects on early growth and cytotoxicity on cell division and chromosomes have been studied. Seeds of Pisum sativum cv. Master B were soaked in AgNPs solutions at concentrations of 20, 40, 80 and 160 mg/L for two hours, control seeds were simultaneously soaked in distilled water. Seeds were then germinated on filter papers moistened with the above concentrations. Seed germination was gradually enhanced at lower concentrations of AgNPs (20 and 40 mg/L) and decreased at higher concentrations (80 and 160 mg/L) compared to control. Seedling growth parameters except root length were all reduced. Deformation of root shape (twisted …

Authors

May Labeeb,Abdelfattah Badr,Soliman A Haroun,Magdy Z Mattar,Aziza S El-Kholy,Ibrahim M El-Mehasseb

Journal

Gesunde Pflanzen

Published Date

2020/6

Comparative analysis of drought stress response of maize genotypes using chlorophyll fluorescence measurements and leaf relative water content

The initial photochemical quantum efficiency of photosystem II (Fv/Fm) and performance index (PI), describing the ability of the photosynthetic apparatus to collect light energy, have been used to screen tolerance to drought stress by ten maize accessions, monitored by leaf relative water content (RWC) and soil water content (SWC). The Fv/Fm, PI, and RWC values were significantly reduced in drought-stressed plants. The analysis of chlorophyll a fluorescence induction rise from the basic dark-adapted fluorescence yield to the maximum (OJIP transient), distinguished accession Zea 1006 from Libya and Zea 612 from Italy, as the most tolerant and the least tolerant genotypes. The maize genotypes were classified using the Principal Component Analysis (PCA) and clustering methods, based on Fv/Fm and PI values, leaf RWC and SWC. Genotypes from Egypt and Libya were differentiated from genotypes from Europe, Russia and the USA.

Authors

A Badr,W Brüggemann

Journal

Photosynthetica

Published Date

2020/1/1

Ecofriendly synthesis of silver nanoparticles and their effects on early growth and cell division in roots of green pea (Pisum sativum L.)

The present study deals with the eco-synthesis and effects of silver nanoparticles (AgNPs) on pea (Pisum sativum L.). AgNPs were synthesized by using gelatine/glucose mixture as a reducing/stabilizing agent for silver nitrate. The AgNPs were characterized and their effects on early growth and cytotoxicity on cell division and chromosomes have been studied. Seeds of Pisum sativum cv. Master B were soaked in AgNPs solutions at concentrations of 20, 40, 80 and 160mg/L for two hours, control seeds were simultaneously soaked in distilled water. Seeds were then germinated on filter papers moistened with the above concentrations. Seed germination was gradually enhanced at lower concentrations of AgNPs (20 and 40mg/L) and decreased at higher concentrations (80 and 160mg/L) compared to control. Seedling growth parameters except root length were all reduced. Deformation of root shape (twisted, folded and hocked roots) was induced upon exposure to AgNPs. Cytologically, mitotic index declined, and chromosomal abnormalities raised as the concentration of AgNPs increased. Observed abnormalities comprised disturbed mitotic phases and cladistic aberrations such as chromosome bridges, rings, breaks, and micronuclei indicating a genotoxic potential for the AgNPs at high concentrations.

Authors

May Labeeb,Abdelfattah Badr,Soliman A Haroun,Magdy Z Mattar,Aziza S El-Kholy,Ibrahim M El-Mehasseb

Journal

Gesunde Pflanz

Published Date

2020/6/1

Effect of L-carnitine on Recovery and Maturation Rates and Quality of Embryos Produced from Vitrified Immature Bovine Oocytes

The present study aimed to assess the effects of supplementation of the vitrification solution (VS) with L-carnitine (LC) on post-thawing recovery and maturation rates of immature bovine oocytes obtained from slaughterhouse ovaries. Immature oocytes (N= 702) were divided into six groups for vitrification. Oocytes of group one (N= 118) were vitrified in 40% dimethyle sulphoxide (40% DMSO; VS1), the second group included 105 oocytes which were vitrified in 40% ethylene glycol (40% EG; VS2). The previous two VS were used in the vitrification of group 3 (N= 98), meanwhile oocytes of group 4 (N= 98) were exposed to VS1+ VS2 supplemented with 5 mM LC. In group 5 (145), oocytes were vitrified as those of group 4 but the VS was supplemented with 10 mM LC, while oocytes in group 6 (N= 138) were vitrified in VS1+ VS2 supplemented with 15 mM LC. After thawing, viable COCs were submitted to in vitro maturation. The differences in recovery rates and maturation rates were compared using chi square analysis. The study revealed important results and cleared the protective effects of LC against oxidative damage provoked by vitrification. A significantly higher recovery rate was observed for COCs vitrified in media containing LC. Recovered COCs in LC groups achieved higher maturation rates (79 and 81.25% for LC groups 10 and 15 mM, respectively), compared to those recovered from un-supplemented vitrification media (27.27 and 45.00%, for DMSO and EG groups, respectively). When matured oocytes were submitted to in vitro fertilization and culture, the morula-blastocyst rates were high in all groups (55.56 to 85.71%). The addition of …

Authors

A Badr,E Abdel-Gawad,B Abdel-Halim,T Mohamed,R Abdel

Journal

Curr. Sci. Int

Published Date

2020

Special issue in honour of Prof. Reto J. Strasser–Comparative analysis of drought stress response of maize genotypes using chlorophyll fluorescence measurements and leaf …

The initial photochemical quantum efficiency of photosystem II (F v/F m) and performance index (PI), describing the ability of the photosynthetic apparatus to collect light energy, have been used to screen tolerance to drought stress by ten maize accessions, monitored by leaf relative water content (RWC) and soil water content (SWC). The F v/F m, PI, and RWC values were significantly reduced in drought-stressed plants. The analysis of chlorophyll a fluorescence induction rise from the basic dark-adapted fluorescence yield to the maximum (OJIP transient), distinguished accession Zea 1006 from Libya and Zea 612 from Italy, as the most tolerant and the least tolerant genotypes. The maize genotypes were classified using the Principal Component Analysis (PCA) and clustering methods, based on F v/F m and PI values, leaf RWC and SWC. Genotypes from Egypt and Libya were differentiated from genotypes from …

Authors

A Badr,W Brüggemann

Journal

Photosynthetica

Published Date

2020/5/28

Screening for Drought Tolerance in Maize (Zea mays L.) Germplasm Using Germination and Seedling Traits under Simulated Drought Conditions

Drought stress is an abiotic factor affecting growth badly and ultimately, yields of crop plants. The current study was planned to explore the variations and determine the performance of target traits under drought conditions. Six seedling characters; that is, number of crown roots, number of seminal roots, primary root length, number of lateral roots, fresh root weight and dry root weight were evaluated under three moisture levels. On the basis of mean values, hybrids of tropical yellow H3, H4, H8, H11, H15, H19 and highland yellow H27, H29 showed best performance under the drought conditions. Principal component analysis was also used to assess the contribution of major traits which were attributing maximum variations among maize hybrids. The first two components with eigen values greater> 1 contributed 76.94% of the variability among the hybrids. The PC-1 was related to the number of seminal roots while the PC-2 was related to the number of crown roots and number of seminal roots. The magnitude of broad sense heritability was high for all the traits. It suggested that all traits were genetically determined and there is an ample scope for the improvement of these traits by selection and breeding.

Authors

Abdul Qayyum,Shahzad Ahmad,Shoaib Liaqat,Waqas Malik,Etrat Noor,Hafiz Muhammad Saeed,Memoona Hanif

Journal

African Journal of Agricultural Research

Published Date

2012/6/26

STABILITY OF SOME COMMON BEAN CULTIVARS UNDER DIFFERENT PLANT DENSITIES

A field experiment was carried out during two successive summer season at years 2016 and 2017 at privet farm, in Al-Rahmania district, El-Behira governorate, Egypt, to examine the magnitude of genotype-environment interactions over six different environments. The five commercial cultivars of common bean (Paulista, Valentino, Bronko, Nebraska, and Giza-6) were evaluated under six environments, i.e., combinations of one and two planting sides with densities (25, 20 and 15 cm). Pooled analysis of variance over all densities (environments) displayed significant to highly significant differences between genotypes and environment relative to studied traits, while significant genotypes × environment (G×E) were shown for each of plant height (cm), number of leaves/plant, number of pods/plant, pod yield/m2 and pod yield/feddan in both seasons as well as leaf area and each of number of branches, total chlorophyll and pod diameter in 1st and 2nd season, respectively indicating that genotypes responded differently to various environments which indicated a wide range of variability among the genotypes performance. The value of Regression coefficient “bi” approached nearly unity in some genotypes for some traits, indicating average response to the fluctuating environmental conditions prevailed the different densities across both seasons. On the other hand, “bi” value was more than one (bi>1) for some genotypes and on contrary, regression coefficient was less than 1 (bi<1) for 4 genotypes at least two to six studied traits in both seasons. Conclusively,  high potential response for Nebrasca genotype in favorable environments with adequate …

Authors

Abd El Fattah Badr

Journal

Journal of Productivity and Development

Published Date

2020/10/1

Genetic diversity and population structure of the medicinal plant Achillea fragrantissima (Forssk.) Sch. Bip. in the mountains of South Sinai, Egypt

The Mediterranean sesame core collection contains agro-morphologically superior sesame accessions from geographically diverse regions in four continents. In the present investigation, the genetic diversity and population structure of this collection was analyzed with 5292 high-quality SNPs discovered by double-digest restriction site associated DNA (ddRAD) sequencing, a cost-effective and flexible next-generation sequencing method. The genetic distance between pairs of accessions varied from 0.023 to 0.524. The gene diversity was higher in accessions from Asia than from America, Africa, and Europe. The highest genetic differentiation was observed between accessions collected from America and Europe. Structure analysis showed the presence of three subpopulations among the sesame accessions, and only six accessions were placed in an admixture group. Phylogenetic tree and principal coordinate analysis clustered the accessions based on their countries of origin. However, no clear division was evident among the sesame accessions with regard to their continental locations. This result was supported by an AMOVA analysis, which revealed a genetic variation among continental groups of 5.53% of the total variation. The large number of SNPs clearly indicated that the Mediterranean sesame core collection is a highly diverse genetic resource. The collection can be exploited by breeders to select appropriate accessions that will provide high genetic gain in sesame improvement programs. The high-quality SNP data generated here should also be used in genome-wide association studies to explore qualitative trait loci and SNPs …

Authors

Merve Basak,Bulent Uzun,Engin Yol

Journal

PLoS one

Published Date

2019/10/10

Genetic Diversity among Selected Medicago sativa Cultivars Using Inter-Retrotransposon-Amplified Polymorphism, Chloroplast DNA Barcodes and Morpho …

Alfalfa (Medicago sativa L.) is a major forage crop of family Fabaceae and is frequently cultivated in Egypt. The present study is concerned with the genetic discrimination of fifteen alfalfa cultivars from three different countries (Egypt, Australia, and USA) using two molecular approaches: inter-retrotransposon-amplified polymorphism (IRAP) markers and two chloroplast DNA barcodes matK and the trnH in addition to the analysis of fifteen morpho-agronomic traits. The genetic relatedness, based on analysis of IRAP marker polymorphism and produced using eleven primers by clustering via principal component analysis (PCA) and multivariate heatmap biostatistical methods differentiated the two Egyptian cultivars EGY1-Ismailia1 and EGY2-Nubaria1 from the three Australian and seven American cultivars, with some distinction of the cv. USA6-SW9720 and cv. AUS4-SuperFast. The results were also supported by the sequence analysis of the matK and the trnH genes on the genetic relatedness between eight cultivars. Moreover, it might be suggested that breeding lines from M. sativa cultivars may provide novel insights and a better understanding of the domestication of M. sativa genetic diversity. The classification of the eight cultivars, as revealed by morpho-agronomic traits, confirmed the close genetic relationship between the two Egyptian cultivars and indicated some resemblance between them and the AUS2-Siri Nafa, whereas the two American cultivars, USA1-Super supreme and USA4-Cuf101, were clearly isolated from a cluster of other three cultivars USA7-SW9628, USA8-Magna901, and USA9-Perfect. The results are useful sources of …

Authors

Abdelfattah Badr,Nahla El-Sherif,Sara Aly,Shafik D Ibrahim,Mohamed Ibrahim

Journal

Plants

Published Date

2020/8/5

Role of salicylic acid in biotic and abiotic stress tolerance in plants

Salicylic acid (SA) is an endogenous growth regulator of phenolic nature and also a signaling molecule, which participates in the regulation of physiological processes in plants such as growth, photosynthesis, and other metabolic processes. Several studies support a major role of SA in modulating the plant response to various biotic and abiotic stresses. Its role in plant disease resistance is well documented for dicotyledonous plants, where it is required for basal resistance against pathogens as well as for the inducible defense mechanism and systemic acquired resistance (SAR); this confers resistance against a broad spectrum of pathogens. The activation of SAR is associated with the heightened level of expression of the pathogenesis-related proteins, some of which possess antimicrobial activity. Also, SA potentially generates a wide array of metabolic responses in plants and also affects plant-water relations …

Authors

Heba I Mohamed,Hanaa H El-Shazly,Abdelfattah Badr

Journal

Plant Phenolics in Sustainable Agriculture: Volume 1

Published Date

2020

abdelfattah badr FAQs

What is abdelfattah badr's h-index at Helwan University?

The h-index of abdelfattah badr has been 18 since 2020 and 30 in total.

What are abdelfattah badr's top articles?

The articles with the titles of

Genome editing by site-directed nucleases and its applications in producing climate change resilient crop plants

ISSR Markers Related to Effects of Gamma Irradiation on Growth and Yield Parameters of Fenugreek (Trigonella foenum-graecum L.)

Growth, physiological, and molecular responses of three phaeophyte extracts on salt-stressed pea (Pisum sativum L.) seedlings

Genetic diversity of Citrullus colocynthis populations using phytochemical analysis and SCoT marker variations

Gamma-irradiation Induces Phenotypic and Genotypic Mutations in M3 and M4 Generations of Phaseolus vulgaris

Genetic diversity analysis in wheat cultivars using SCoT and ISSR markers, chloroplast DNA barcoding and grain SEM

Ultrastructural and molecular implications of ecofriendly made silver nanoparticles treatments in pea (Pisum sativum L.)

Chromosomes as Sources of Taxonomic Information for Plant Systematics and Evolution

...

are the top articles of abdelfattah badr at Helwan University.

What are abdelfattah badr's research interests?

The research interests of abdelfattah badr are: plant science

What is abdelfattah badr's total number of citations?

abdelfattah badr has 3,612 citations in total.

What are the co-authors of abdelfattah badr?

The co-authors of abdelfattah badr are William Martin, Ruediger Cerff.

    Co-Authors

    H-index: 104
    William Martin

    William Martin

    Heinrich-Heine-Universität Düsseldorf

    H-index: 37
    Ruediger Cerff

    Ruediger Cerff

    Technische Universität Braunschweig

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