Abdelbary Prince

Abdelbary Prince

Cairo University

H-index: 15

Africa-Egypt

Abdelbary Prince Information

University

Cairo University

Position

Faculty of Veterinary Medicine

Citations(all)

515

Citations(since 2020)

384

Cited By

217

hIndex(all)

15

hIndex(since 2020)

12

i10Index(all)

18

i10Index(since 2020)

14

Email

University Profile Page

Cairo University

Abdelbary Prince Skills & Research Interests

Genomics and Proteomics

Top articles of Abdelbary Prince

The puzzling etiologies of transient black discoloration in Nile Tilapia (Oreochromis niloticus) intensively cultured under RAS system

The aim of this study is to identify the hidden etiologies of the black chromo-shifting transient phenomenon affecting Nile tilapia, Oreochromis niloticus, farmed in RAS-controlled ponds together with the assessment of the immunological reaction against the chronic irritating effects of the invasive agent. A total of 100 Nile Tilapia were collected from a private farm at Kafrelsheikh Province, located on the northern side of the Egyptian Nile Delta. The clinical history of the affected fish farm showed an unknown chromo-shifting phenomenon where tilapias were exhibiting severe black skin coloration, which gradually disappeared after removal from the tank’s water. A comprehensive gross examination of the collected fish; including parasitological examination of skin and gill scraps, was performed. Blood biochemical testing was performed on the infested blackish O. niloticus and control non-infested fish. The current study …

Authors

Alaa Eldin Eissa,Marwa M Attia,Rabia A El Zlitne,Ayad A Magdy,Asmaa Edrees,Mahmoud S Sharaf,Abeer E Mahmoud,Awad A Abdelbaky,Rehab R Abd ElMaged,Elshaimaa Ismael,Rehab A Qorany,Doaa F El Moghazi,Abdelbary Prince,Emad A Afiffy,Said El Behiri,Nehal A Younis

Journal

Aquaculture International

Published Date

2024/2

Efficiency of xylanase, emulsifier, and guanidinoacetic acid in restoring energy deficit in male broilers fed low metabolisable energy diets

The study aimed to examine the distinct impacts of incorporating xylanase (Xyl), emulsifier (EM), and guanidinoacetic acid (GAA) as dietary supplements in low metabolisable energy (ME) diets on performance, energy and protein efficiency ratios, oxidative biomarkers, gene expression, and gut morphology. Seven hundred one-day-old (Ross 308) male-broilers were assigned to 5 dietary treatments with 5 replicates of 28 birds each. The experimental group denoted as the positive control (PC) fed on diets in accordance with the breed recommendations. The negative control (NC) was subjected to a dietary intervention reducing the ME by 200 kcal/kg compared to the PC. The remaining experimental diets comprised NC diet that were supplemented with 0.01% Xyl, 0.03% EM and 0.06% GAA. Results showed that birds fed low-ME-diets increased their voluntary feed intake to meet their energy needs but was at the expense of their productive efficiency. Only NC+ GAA partially restored broiler performance compared to PC. However, compared to the PC group Xyl, EM and GAA improved the energy and growth-related gene expression, oxidative biomarkers, and gut histomorphology (p< 0.05). The key features associated with Xyl and EM were growth-related genes and intestinal mucus, while GAA was associated with energy-related genes, oxidative biomarkers and jejunum-villi height and villus: crypt ratio. In conclusion, Xyl, EM and GAA supplementation to NC group were able to improve the health status of the birds. However, to improve the production efficiency, future research is needed to elucidate the effect of combined products in birds fed on …

Authors

Shady Khalil,Hossam Abdellatif,Ahmed Al-Sagan,Tarek Melegy,Abdelbary Prince,Ramadan El-Banna

Journal

Journal of Advanced Veterinary Research

Published Date

2024

Comparative Study between Mushroom-extracted and Commercial Lectins: Impact of Immune Response to H5N1, NDV, and IBD Vaccines in Broiler Chicken

This study's objective was to look into the impact of lectin on the immune response to live and inactivated vaccines of Avian influenza, Newcastle disease virus, and Gumboro in broiler chicken. Moreover, a comparison between mushroom-extracted lectin and commercial lectin is investigated. Accordingly,100 broiler chicken were divided into 4 groups each one consisting of 25 chicken: the first group is -ve control, and the second one was treated with extracted lectin only. Followed by a third group, which is treated with commercial lectin only. Finally, the fourth group was treated with both extracted and commercial lectins. The vaccination program was VAXXITEK® HVT+IBD in the hatchery, then ValleyVac® Penta-Pro injection on the sixth day, and Nobilis® Gumboro 228E on the 16th day in drinking water. Lectin dose is 0.25 ml/L of drinking water for 2 days before live vaccines and 2 days after inactivated vaccines. After that, samples were collected before and after 48 hrs lectin administration in case of Infectious Bursal Disease (IBD). Otherwise, samples were taken 48 hrs before and 10 days after lectin administration in case of Highly Pathogenic Avian Influenza (H5N1) and Newcastle Disease Virus (NDV). Then, 3 samples from each group in each measurement are extracted for testing. The results obvious that there is no change in immune response in four groups to the Newcastle vaccine before and after lectin, indicating no effect of lectin on NDV immunity. Additionally, the titre of avian influenza after lectin administration is the same as before lectin, indicating no effect of lectin on H5N1. On the other hand, there is a superior impact of mushroom …

Authors

Abdelrahman MF Darwish,Abdelbary Prince,Yakout A El Senosi,Hassan SI Alharthy

Journal

Benha Journal of Applied Sciences

Published Date

2024/3/12

Effects of phytase enzyme supplementation on growth performance, intestinal morphology and metabolism in Nile tilapia (Oreochromis niloticus)

Phytase is crucial in enhancing the bioavailability and release of phosphorus and other nutrients bound to phytic acid, making them more bioavailable for animal absorption. This study was carried out to inspect the effect of supplementing low phosphorus (P) diet with di‐calcium phosphate (DCP) and liquid phytase enzyme (LP), which contains 1500 FTU/kg, on growth performance, intestinal morphometry, proximate body chemical composition, blood profile, immunity status, liver mitochondrial enzyme activities, the expression response and economic returns of Nile tilapia (Oreochromis niloticus). Three triplicate groups of fish (initial weight 5.405 ± 0.045 g, N = 90) were fed on three different diets for 90 days. The first was a control diet with zero DCP; the second was a control diet supplemented with 0.71% DCP; the third was a control diet supplemented with 0.03% LP. The groups were designated as CG, DCP …

Authors

Ahmed E Negm,Mohamed H Abo‐Raya,Asmaa M Gabr,Samar H Baloza,Asmaa El‐Nokrashy,Abdelbary Prince,Daniel Arana,Youji Wang,Shimaa Abdelazeem,Ghadeer M Albadrani,Muath Q Al‐Ghadi,Ahmed Abdeen,Mustafa Shukry,Malik M El‐Sayed Khalafallah

Journal

Journal of Animal Physiology and Animal Nutrition

Published Date

2024/2/14

Biochemical and Molecular Identification of the Most Common Streptococci Affecting Common pandora (Pagellus erythrinus Linnaeus, 1758) From the Mediterranean Coast of Tripoli

Streptococcosis is one of the septicaemic bacterial diseases of public health concern due to its zoonotic potential. Its luxurious existence in wild marine fish from an open water body strongly suggests presence of massive sewage pollution. The aim of the current study was to identify and characterize most common streptococci  affecting common pandora (Pagellus erythrinus) inhabiting the Mediterranean coast of Tripoli. A total number of 270 common pandora  were clinically examined for possible streptococcal infection and non-streptococcal infection. The fishes were collected from the area extending from Tripoli to Tajoura (east to Tripoli) during the three seasons, summer, autumn, and spring. No fish samples were available during winter due to the bad climate / storms along the entire western Libyan Mediterranean coasts. Streptococcus iniae, Streptococcus dysgalactiae, Streptococcus phocae, Enterococcus faecalis, and other non-streptococcal species, such as Aeromonas hydrophila, Pseudomonas anguilliseptica, and Photobacterium damselae sub species damselae were biochemically identified. Regardless of the season, S. iniae and E. faecalis were the most prevalent streptococcal species (13% and 8.88%, respectively). In contrast, the most retrieved non-streptococcal species were A. hydrophila, followed by Pseudomonas anguilliseptica, with a prevalence of 10%, and 14.44%, respectively. The highest infections were recorded during autumn, followed by summer, then spring with percentages of 53.34%, 50%, and 43.4%, respectively. The majority of the isolates were sensitive to erythromycin, florfenicol, and sulfamethoxazole …

Authors

Alaa Eldin Eissa,Abdulatif A Asheg,Abdulsalam Abu Mhara,Mahmoud Sharaf,Awad A Abdelbaky,Amira SA Attia,Tarek D Dakhil,Alkhateib Y Gaafar,Eman M Ismail,Rasmia HM Abu Leila,Heba A Abdel Hady,Emad A Afify,Abdelbary Prince,Reham H Ragab,Khalid Shahin

Journal

Journal of Applied Veterinary Sciences

Published Date

2024/2/1

International Journal of Veterinary Science

This study aimed to determine the effect of using ammoniated Cymbopogon nardus waste (ACNW) as a substitute for grass on growing male Ettawa Crossbreed dairy goats (ECDG). A total of eighteen male ECDGs aged between 24-30 months with a body weight of 26.4±1.01 kg were used in this study. The study used a Randomized Block Design (RBD) which consisted of three treatments and six replication. Treatments were A= 40% native grass+ 60% concentrate, B= 30% native grass+ 10% ammoniated C. nardus waste (ACNW)+ 60% concentrate, and C= 20% native grass+ 20% ACNW+ 60% concentrate. Parameters observed were nutrient intake, digestibility of nutrients, and body weight gain of ECDG. The results showed that the treatment did not have a different effect on nutrient intake and digestibility of nutrients, body weight gain, and ration efficiency in male ECDGs that were given ammoniated C. nardus as a substitute for grass. Nutrient intake for each treatment were A= 1.13 kg/h/d, B= 1.09 kg/h/d, C= 1.05 kg/h/day. Digestibility of dry matter were A= 77.85%, B= 76.47%, C= 76.46%. Daily weight gain were A= 135g/d, B= 122g/d, C= 118g/d. The conclusion is the use of ammoniated C. nardus waste of up to 50% as a grass substitute can be used for fattening Ettawa Crossbreed dairy goat.

Authors

Rusmana Wijaya Setia Ningrat,Mardiati Zain,Erpomen Elihasridas,Windu Negara,Ezi Masdia,Roni Pazla Putri,Putri Okta Shafura,Ummi Amanah

Journal

Int J Vet Sci

Published Date

2024

Occurrence of toxigenic Clostridium difficile among diarrheic sheep and goats in rural settings: public health concern.

Clostridium difficile is a global pathogen with great public health concern. This study was conducted to inquire into the presence of C. difficile amongst diarrheic sheep and goats in rural environments. Fecal samples were collected from 60 diarrheic animals (36 sheep and 24 goats). Samples were cultivated for isolation of C. difficile using selective medium while suspected colonies were confirmed by both serological and molecular techniques. Afterwards, toxigenic C. difficile isolates were recognized using PCR after finding tcdA and tcdB genes encoding toxin A and toxin B, respectively. The overall incidence of C. difficile was 20%(12/60) whereas the prevalence rates were 19.4% and 20.8% for sheep and goats, respectively. Only 3/12 isolates were toxigenic, two isolates (one from sheep and another from a goat) were tcdA-positive, but only one isolate from sheep was tcdB-positive. The phylogenetic analysis of the obtained tcdA gene sequence from sheep revealed that this sequence was grouped in the same clade with those isolated from beef, pig and human case. In conclusion, the current study highlights the occurrence of toxigenic C. difficile strains among diarrheic sheep and goats, a matter which has a high concern in both veterinary medicine and public health.

Authors

Mohamed Fathy,Khaled A Abdel-Moein,Wafaa A Osman,Ahmed M Erfan,Abdelbary Prince,Alaa A Elgabaly,Adel M Elkattan,Ahmed Samir

Published Date

2023/7/11

Impact of Anisakis pegreffi Infection on Gonadal Health and Gonadosomatic Index of European Hake (Merluccius merluccius)

Parameters belonging to the physical status and gonadal size of certain fish provide crucial information for assessing both the productivity and fecundity of declining fish populations. These parameters are vulnerable to the negative impacts of disease agents such as internal or systemic parasites. Although parasitic diseases might influence these parameters, the literature investigating these pathophysiological alterations is scanty. Therefore, the current study represents one of the scarcest studies that document the possible link between parasitism, gonadal health, and the growth of European hake (Merluccius merluccius). Screening of imported European halves indicated a relatively high prevalence of Anisakis pegreffi Larvae 3 infestation, with an 80% prevalence rate, a mean intensity of 24.4, and a mean abundance of 19.5. However, the prevalence of Anisakid larvae infection in native fish was 36% with a mean intensity of 7.36 and mean abundance of 2.65.The current research revealed remarkable ovarian pathology that involved several forms of degenerative changes in ovarian tissues. Such gonadal pathologies were attributed to the damaging effect of the retrieved Anisakis pegreffi Larvae 3. Gonadosomatic index of both heavily infected imported / native hakes was relatively impacted by the progressive gonadal pathology resulting from Anisakis pegreffi L3 infection. Morphometric measurements of the gonads and body have revealed that, gonadosomatic index of both heavily infected imported / native hakes was relatively impacted by the progressive gonadal pathology resulting from Anisakis pegreffi L3 infection.

Authors

Alaa Eldin Eissa,Reda M Korany,Rabia A El Zlitne,Ayad Magdy,Mahmoud S Sharaf,Awad A Abdelbaky,Abeer E Mahmoud,Rehab Abd ElMaged,Rehab A Qorany,Asmaa MK Mohamed,Doaa M Faisal,Ebtehal E Hussein,Hatem H Mahmoud,Hisham M Elgazzar,Said El Behiri,Emad Afify,Abdelbary Prince,Marwa M Attia

Journal

Journal of Applied Veterinary Sciences

Published Date

2023/7/1

Molecular screening of the most common streptococci affecting Common pandora (Pagellus erythrinus Linnaeus, 1758) caught from the Mediterranean coast of Tripoli

A total number of samples, 270 Common pandora (Pagellus erythrinus), were clinically examined for possible streptococcal infection. The fishes were collected from the area extending from Tripoli to Tajoura (east to Tripoli). S. iniae, S. dysgalactiae, S. phocae, Enterococcus fecalis, and other non-streptococcal spp. Such as Aeromonas hydrophila, Pseudomonas, and Photobacterium damselae were biochemically identified. 90 fish per season were thoroughly screened for streptococcal infection for three successive seasons. Regardless of the season, S. iniae and E. fecalis were the most isolated streptococcal spp (13% and 8.88%, respectively). In contrast, the most retrieved non-streptococcal spp were A. hydrophila, followed by Pseudomonas spp, with isolation percentages of 10% and 14.44%, respectively. The highest infections have occurred during winter, followed by summer, then spring seasons with isolation percentages of 53.34%, 50%, and 43.4%, respectively. The majority of the isolated bacterial spp were sensitive to Erythromycin, Florfenicol, and sulfamethoxazole-Trimethoprim. Molecular screening utilizing the sequences of 16S rRNA genes has confirmed the phylogenetic relationship to S. dysgalactiae, E. faecalis, S. iniae, and S. phocae isolates with similarity percentages exceeding 99.6%. The sequences were deposited in the GenBank with accession numbers (OK033868, OK033869, OK033870, and OK033871).

Authors

Alaa Eldin Eissa,Abdulatif A Asheg,Mahmoud S Sharaf,Awad A Abdelbaky,Abdelsalam Abu Mhara,Tarek D Dakhil,Alkhateib Y Gaafar,Eman M Ismail,Said El Behiri,Emad A Afiffy,Abdelbary Prince,Khalid Shahin

Published Date

2023/4/12

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

BackgroundMultiple sclerosis (MS) is the most frequent non-traumatic neurological debilitating disease among young adults with no cure. Over recent decades, efforts to treat neurodegenerative diseases have shifted to regenerative cell therapy. Adipose tissue-derived stromal vascular fraction (SVF) comprises a heterogeneous cell population, considered an easily accessible source of MSCs with therapeutic potential in autoimmune diseases. This study aimed to assess the regenerative capacity of low-level laser-activated SVF in an MS cat model.MethodsFifteen adult Persian cats were used in this study: Group I (control negative group, normal cats), Group II (EB-treated group, induced for MS by ethidium bromide (EB) intrathecal injection), and Group III (SVF co-treated group, induced for MS then treated with SVF on day 14 post-induction). The SVF was obtained after digesting the adipose tissue with collagenase …

Authors

Mariam F Farid,Yara S Abouelela,Noha AE Yasin,Asmaa K Al-Mokaddem,Abdelbary Prince,Marwa A Ibrahim,Hamdy Rizk

Journal

Stem Cell Research & Therapy

Published Date

2023/1/11

A novel cell-free intrathecal approach with PRP for the treatment of spinal cord multiple sclerosis in cats

BackgroundMultiple sclerosis (MS) is a progressive autoimmune demyelinating disease of the central nervous system. To date, there is no effective therapy for it. Our study aimed to determine the potential role of platelet-rich plasma (PRP) in the treatment of MS in cats.MethodsThe current study was conducted on 15 adult Persian cats that were divided into three groups: control negative, control positive (ethidium bromide (EB)-treated group), and PRP co-treated group (EB-treated group intrathecally injected with PRP on day 14 post-spinal cord injury). PRP was obtained by centrifuging blood on anticoagulant citrate dextrose and activating it with red and green laser diodes. The Basso–Beattie–Bresnahan (BBB) scores were used to assess the motor function recovery on days 1, 3, 7, 14, 20, and 28 following 14 days from EB injection. Moreover, magnetic resonance imaging (MRI) analysis, histopathological …

Authors

Mariam F Farid,Yara S Abouelela,Noha AE Yasin,Mohamed R Mousa,Marwa A Ibrahim,Abdelbary Prince,Hamdy Rizk

Journal

Inflammation and Regeneration

Published Date

2022/10/14

Moringa oleifera mitigates oxytetracycline stress in Oreochromis niloticus

Antibiotics serve as the cornerstone of the treatment of bacterial diseases in the aquaculture sector. In this study, 210 Nile tilapias (Oreochromis niloticus) weighing approximately 60 ± 2.5 g b.w. were randomly divided into seven treatments (T1–7). The fish were experimentally infected with Aeromonas hydrophila and then treated with oxytetracycline (OTC) after the appearance of clinical symptoms. The fish were fed Moringa oleifera diet alone or M. oleifera diet before OTC treatment to alleviate the effects of OTC treatment. The effects of OTC treatment and M. oleifera were assessed by measuring the gene expressions of cytokines (interleukin [IL]‐1β, IL‐6, tumour necrosis factor‐α, IL‐10 and heat shock protein‐70), antioxidant enzymes (catalase, superoxide dismutase, lipid peroxidase and total antioxidant capacity) and liver enzymes (aspartate aminotransaminase, alanine aminotransaminase and alkaline …

Authors

Ahmed H Sherif,Abdelbary Prince,Ahmed Adel Seida,Mahmoud Saad Sharaf,Elsayed A Eldessouki,Mohammed A Harfoush

Journal

Aquaculture Research

Published Date

2022/4

Ameliorative Effect of Some Medicinal Plants Against Experimentally Hyperuricemic Rats

The present study was conducted to investigate changes in expression of xanthine oxidase (XO) enzyme during hyperuricemia and the ameliorating effect of natural safe source as medicinal plants on the hyperuricemia in rats. For this purpose, 50 albino rats were categorized into 5 equal groups as follows:(GI) Control group,(GII) Potassium Oxonate (PO) Hyperuricemic group,(GIII) Febuxostat Hyperuricemic group,(GIV) Gingko biloba hyperuricemic group and (GV) Onion juice hyperuricemic group. At the end of 4 weeks, blood samples were collected and sera were separated and analyzed for determination of xanthine oxidase, ALT, AST, ALP activities and uric acid and total protein concentrations, liver and kidney specimens divided into 2 parts. Part for estimation of MDA, GSH, uric acid contents and SOD, GSH-Px activity and part (liver only) for studying xanthine oxidase gene expression. PO increased XO gene expression and enzyme activity it, uric acid, MDA, ALT, AST, and ALP., while it decreased GSH, SOD, GSH-Px and total proteins. Febuxostat decreased XO gene expression and enzyme activity, uric acid, MDA, GSH, GSH-Px and total proteins but it increased SOD, ALT, AST, and ALP. activities. Both Gingko biloba and onion juice showed a similar influence as Febuxostat but with lower degree in concerning XO gene expression and enzyme activity and uric acid, but they have more potent antioxidative effect than Febuxostat.

Authors

Els T Awad,SA Abdelaziz,AM Prince,IM Ahmed,HA Elsayed

Journal

Journal of Positive School Psychology

Published Date

2022/3/23

Morphomolecular identification of two trichodinid coinfections (Ciliophora: Trichodinidae) and their immunological impacts on farmed Nile Tilapia

Trichodiniasis is one of the most serious ectoparasitic diseases disturbing tilapia aquaculture worldwide, causing deleterious effects and significant economic losses. This study aimed to characterize two Trichodina species concomitantly infecting farmed Nile tilapia Oreochromis niloticus based on integrated morphological, molecular, and immunological approaches. One hundred and eighty of 250 examined Nile tilapia were found to be infected with trichodinids belonging to the family Trichodinidae, with an overall prevalence rate of 72%. The morphological characterization of trichodinids fixed on Giemsa‐stained smears revealed that fish were concomitantly infected with Trichodina heterodentata and T. centrostrigeata. Subsequently, the phylogenetic analysis of the 18S rRNA gene of the two investigated trichodinids confirmed the detected species’ identities. The gene expression levels of MHCIIβ and CD4 gens …

Authors

Marwa M Attia,Mamdouh Y Elgendy,Abdelbary Prince,Mahmoud M El‐Adawy,Mohamed Abdelsalam

Journal

Aquaculture Research

Published Date

2021/9

The international journal of animal biosciences

N. Amiri a, M. Afsharmanesh a,⁎, M. Salarmoini a, A. Meimandipour b, SA Hosseini c, H. Ebrahimnejad d a Department of Animal sciences, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran b Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran c Department of Animal and Poultry Nutrition, Animal Science Research Institute, Karaj, Iran d Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran abstract articleinfo

Authors

D Hamidi,NA Grinnell,M Komainda,F Riesch,J Horn,S Ammer,I Traulsen,R Palme,M Hamidi,J Isselstein

Published Date

2022

Biomedical Activity of Egyptian Hibiscus sabdariffa L. as Modulator of Alzheimer’s Beta-Amyloid1-42 Secretion in a Human Neuroglioma Model

Abstract β-Amyloid1-42, the major constituent of amyloid plaques, was believed to play a central role in the neuropathology of AD according to the ‘‘amyloid cascade hypothesis’’. In our study, we evaluated the in vitro profiles of four Roselle extracts against Aβ42 production. The in-vitro Aβ42 inhibiting activity was evaluated in a human neuroglioma cell line (H4) carrying the double Swedish mutation (K670N/M671L) of the human amyloid precursor protein (APPsw) under the transcriptional control of the hamster prion protein promoter. The in-vitro anti-COX activity was also investigated using human recombinant enzymes isolated from transfected Sf-9 cells. In addition, the cytotoxicity study by standard MTT assay and the antioxidant activity of Roselle extracts using DPPH Free Radical Scavenging Assay was also investigated. All Extracts concentrations showed a good inhibitory activity against DPPH free radical. The antioxidant activity of our extracts as IC50 was in the range of (45.5-88.5 µg/ml) among the four Roselle extracts within the two solvent system compared to 47.5 µg/ml of Gallic acid. No toxicity was detected by standard MTT assay against H4 cells treated with Roselle extracts by concentrations up to 600 ng/ml. A dose dependent inhibition of Aβ42 secretion was observed. Our Roselle extracts were found to be 1.5-and 3-fold more potent than R-flurbiprofen in inhibiting Aβ42 secretion and able to modulate the in vitro Aβ42 secretion without any cytotoxic effect. No inhibition activity was also observed against cyclooxygenase-1 (COX-1) and cyclooxygenase-1 (COX-2) up to 600 ng/ml concentration indicating that the reduction in Aβ42 …

Authors

Sayeda El Meqanen,Mohamed Ahmed Badawy,Abdelbary Prince,Mohamed Aly El-Desouky,Mansour Hamed Gheait

Journal

Egyptian Journal of Chemistry

Published Date

2021/10/1

Morpho-molecular identification of Heterophyes heterophyes encysted metacercariae and its immunological and histopathological effects on farmed Mugil cephalus in Egypt

Molecular and immunological aspects of heterophyid infections in mullets are scanty. This study was initiated to identify heterophyid encysted metacercariae infecting gray mullet Mugil cephalus by morphological and molecular assays and to evaluate its immunological effects on infected mullets. Both farmed fish, from a private farm nearby Lake Manzala in Damietta, Egypt, and wild-caught fish, from the Mediterranean Sea in Alexandria, were investigated. The presence of heterophyid encysted metacercariae was significantly higher (80%) in wild than in farmed fish (30%). Morphological identification of metacercariae showed characteristic features of H. heterophyes. The result of sequences of 28S and ITS2 rDNA regions of Heterophyes EMC was consistent with the morphological data. The gene expression analysis of tumor necrosis factor alpha (TNF-α) and interleukin-10 (IL-10) in tissues of infected fish qRT-PCR …

Authors

Marwa M Attia,Mamdouh Y Elgendy,Mohamed Abdelsalam,Azza Hassan,Abdelbary Prince,Nagla MK Salaeh,Nehal A Younis

Journal

Aquaculture International

Published Date

2021/6

Effects of guanidinoacetic acid supplementation on zootechnical performance and some biometric indices in broilers challenged with T3-Hormone

Heat stress can cause systemic immune dysregulation and threaten the health of broilers. Guanidinoacetic acid (GAA) has been shown to be effective against heat stress, but whether it is beneficial for immunity is unclear.Therefore, the effects of dietary GAA supplementation on the immunity of chronic heat-stressed broilers were evaluated. A total of 192 Arbor Acres male broilers (28-day old) were randomly allocated to 4 treatments: the normal control group (NC, 22°C, ad libitum feeding), the heat stress group (HS, 32°C, ad libitum feeding), the pair-fed group (PF, kept at 22°C and received food equivalent to that consumed by the HS group on the previous day), and the GAA group (HG, 32°C, ad libitum feeding; basal diet supplemented with 0.6 g/kg GAA). Samples were collected on d 7 and 14 after treatment. Results showed that broilers exposed to heat stress exhibited a decrease (P < 0.05) in ADG, ADFI, thymus …

Authors

Xin Li,Jiawei Bian,Tong Xing,Liang Zhao,Jiaolong Li,Lin Zhang,Feng Gao

Journal

Poultry Science

Published Date

2023/12/1

Sun-dried Azolla leaf meal at 10% dietary inclusion improved growth, meat quality, and increased skeletal muscle Ribosomal protein S6 kinase β1 abundance in growing rabbit

Rapidly growing human populations and the increased need for high nutritive value meat in terms of low fat, high protein, and low sodium content are the driving reasons for the increase in rabbit meat production. However, dietary protein alternatives to sustain rabbit meat production, without competing with humans for strategic crops are needed. Therefore, the current study was conducted to investigate the effect of Azolla leaf meal (ALM) as a dietary protein source on growth performance, meat quality, and abundance and activation of Ribosomal protein S6 kinase β1 (p70S6K1), a downstream target of mammalian target of rapamycin signalling pathway and, thus, a key player in the regulation of protein synthesis and muscle mass. For this purpose, 60 weaned male V-Line rabbits were blocked for the initial BW and randomly allotted into four dietary treatments, with 15 replicate per treatment (n = 15/group) as follows …

Authors

AM Abdelatty,MI Mandouh,MR Mousa,HA Mansour,H Ford,IB Shaheed,AA Elolimy,A Prince,MA El-Sawy,HO AbuBakr,M Bionaz

Journal

Animal

Published Date

2021/10/1

Impact of nucleotide enriched diets on the production of gilthead seabream, Sparus aurata fingerlings by modulation of liver mitochondrial enzyme activitity, antioxidant status …

The objective of this study was to evaluate the effect of fish meal replacement with a mixture of plant, and animal protein supplemented with a commercial nucleotide NucleoforceFish™, on growth, feed utilization, whole-body composition, biometric indices; liver mitochondrial enzyme activities, selected gene expression and influence on the gut microbiome of gilthead seabream, Sparus aurata. Six hundred and thirty gilthead seabream with an average initial body weight of 0.358 ± 0.002 g/fish, were used. Six experimental diets were formulated to be isonitrogenous, 45% crude protein (CP) and isocaloric 20 MJ/kg gross energy (GE). The control diet (D1) contained 25% of fish meal (FM25) and had no NucleoforceFish™ added (FM25/0), diets 2-3 contained FM at a level of 25% and supplemented with 250 (FM25/250) and 500 (FM25/500) mg/kg NucleoforceFish™, respectively. Diets 4-6 were a non-fishmeal …

Authors

S.J. Davies

Journal

Aquaculture

Published Date

2021

Streptococcus, Centrocestus formosanus and Myxobolus tilapiae concurrent infections in farmed Nile tilapia (Oreochromis niloticus)

Stress triggered concurrent microbial/parasitic infections are prevalent in earthen pond based farmed Nile tilapia Oreochromis niloticus. In the current study, a total of thirty five O. niloticus were collected from a commercial fish farm with a history of severe mortalities at Port Said, Egypt. Nile tilapia samples were subjected to bacteriological, parasitological and pathological examinations. Twenty one Enterococcus fecalis and 15 Streptococcus agalactiae isolates were presumptively identified utilizing the semi-automated API 20 Strept test kit. The identities of the retrieved bacteria were confirmed by the sequencing of 16 S rRNA gene. Moribund O. niloticus were found to be heavily infected by one or both of Centrocestus formosanus encysted metacercariae (EMC) and/or Myxobolus tilapiae spores presenting a unique form of synergistic and/or symbiotic relationship. The identities of both parasites were confirmed …

Authors

Alaa Eldin Eissa,Marwa M Attia,Mamdouh Y Elgendy,Gehad A Ismail,Nader M Sabry,Abdelbary Prince,Mahmoud A Mahmoud,Ghada O El-Demerdash,Mohamed Abdelsalam,Hassan IM Derwa

Journal

Microbial Pathogenesis

Published Date

2021/9/1

Erratum to'Impact of nucleotide enriched diets on the production of gilthead seabream, Sparus aurata fingerlings by modulation of liver mitochondrial enzyme activitity …

The publisher regret the journal place the data of Table 3 in the place of table Number 5 & 6. Please place the right data of Tables 5 & 6 in their proper place instead of the data of Table 3.(See Tables 1-8.)

Authors

AM El-Nokrashy,RA El-Banna,BM Edrise,MM Abdel-Rahim,M Jover-Cerdá,A Tomás-Vidal,A Prince,SJ Davies,ER El-Haroun,AMA-S Goda

Journal

Aquaculture

Published Date

2021/5

Performance of different laboratory methods for detection of clostridium difficile in animal samples

Clostridium difficile is a well-known enteric pathogen causing antibiotic-associated diarrhea and pseudomembranous colitis among humans. Lately, C. difficile has emerged to cause enteric problems in food producing animals, horses and household pets particularly young ones. This study was conducted to investigate the performance of different laboratory diagnostic methods for Clostridium difficile in veterinary field. For this purpose, ninety fecal samples collected from diarrheic sheep, goats and chickens, were examined for the detection of C. difficile using three laboratory methods: direct Polymerase chain reaction (PCR) on DNA extracted from fecal samples, conventional culture followed by molecular confirmation of isolates and glutamate dehydrogenase (GDH) ELISA on feces. The detection rates of C. difficile were 45.6%, 16.7% and 8.9% by direct PCR, conventional culture followed by molecular confirmation of isolates and GDH-ELISA, respectively. Direct PCR yielded the highest detection rate, however, false negative results were recorded in 3 samples being positive by culture method, whereas, all GDH-ELISA positive samples were also positive by the other techniques. In conclusion, Direct PCR on DNA extracted from fecal samples of animals showed the highest detection rate nevertheless false negative results cannot be ruled out.

Authors

M Fathy,KA Abdel-Moein,WA Osman,MA Erfan,A Prince,AA Hafez,HE Mahmoud,TE Mosallam,A Samir

Journal

Adv. Anim. Vet. Sci

Published Date

2021

CRISPR/Cas9-mediated activation of CDH1 suppresses metastasis of breast cancer in rats

BackgroundCancer is a life-threatening disease that affects approximately 18 million individuals worldwide. Breast cancer is the most common female neoplasm globally with more than 276,480 new cases of invasive breast cancer expected to be diagnosed in women in the U.S. alone in 2020. Genetic and epigenetic factors play role in the carcinogenesis and progression of this disease. In this study, MCF-7 adenocarcinoma cells were transfected with CRISPR/Cas9 plasmid to either knock out CDK11 or to activate CDH1. Treated cells were allografted into the mammary glands of female rats (150–190 g, 6–8 weeks) to evaluate the capability of these cells to control cancer progression and metastasis.ResultsqPCR data revealed a significant downregulation of CDK11 and upregulation of CDH1. Cell cycle analysis and apoptosis assays indicated the knockout of CDK11 and simultaneous activation of CDH1 …

Authors

Fatma Al-Mulhim,Amany I Alqosaibi,Afnan Al-Muhnna,Khalid Farid,Shaimaa Abdel-Ghany,Hamdy Rizk,Abdel-Bary Prince,Adaugo Isichei,Hussein Sabit

Journal

Electronic Journal of Biotechnology

Published Date

2021/9/1

Catastrophic mass mortalities caused by Photobacterium damselae affecting farmed marine fish from Deeba Triangle, Egypt

Farmed marine fish constitute 20% of the total farmed fish production in Egypt, and the Deeba Triangle produces a relatively large portion of this percentage. Last year, several private fish farms in the Deeba Triangle have suffered severe economic losses due to acute fish mass kills. This study aimed to investigate the hidden aetiologies behind these colossal mass fish kills and to propose an emergency control strategy. Several tons of dead farmed fish were remarkably scattered throughout affected ponds and at the vicinity of impacted fish farms. Moribund farmed European seabass (Dicentrarchus labrax), thin‐lipped grey mullet (Liza ramada) and gilthead seabream (Sparus aurata) have exhibited skin darkness, emaciation, congested gills and fins, ascites, skin erosions and ulcerations. Internally, moribund fish emitted unpleasant odour upon opening the abdomen together with severe congestion and …

Authors

Alaa Eldin Eissa,Mahmoud Abou-Okada,Abdul Raouf M. Alkurdi,Rabia A. El Zlitne,Abdelbary Prince,Mohamed Abdelsalam,Hassan I. M. Derwa

Journal

Aquaculture Research

Published Date

2021

Morphological, molecular and clinical assessment of different Anisakis species infecting horse Mackerel Trachurus trachurus from South Mediterranean

This study aimed to identify and characterize the most common Anisakis larvae infecting Atlantic horse Mackerel (Trachurus trachurus) in the South Mediterranean basin; based on morphological and advanced molecular characterization. Also, the linkage between the prevalence of Anisakis spp. larvae and some biological variables including Mackerel age and sex were also investigated. Moreover, the clinical intensity of larvae infection in different organs of infected Mackerel was assessed. Atlantic horse mackerel collected from the southern Mediterranean shores were found to be infected with third stages larvae (L3) of Anisakis species. The Anisakis larvae were found encapsulated as coiled tightly in different fish organs and uncoiled freely in the abdominal cavity of infected T. trachurus. These larvae were categorized into 3 types of Anisakis spp. as A. simplex type1; A. pegreffii; Anisakis simplex / pegreffii hybrid based on their morphological and molecular characterizations. Subsequently, the phylogenetic analysis of ITS region of different investigated larvae confirmed the identification of collected Anisakis spp. The remarkably high intensity of Anisakis spp. larvae in Mackerel gonads warns of possible future deleterious impacts on the growth, development and sustainability of Mackerel fisheries at the south Mediterranean coasts.

Authors

Alaa Eldin Eissa,Mohamed Abdelsalam,Marwa M Attia,Gehad A Ismail,Rehab A Qorany,Abdelbary Prince,DA ABDEL-MONEAM

Journal

Egyptian Journal of Aquatic Biology and Fisheries

Published Date

2021

Effect of gingko biloba and onion juice on xanthine oxidase enzyme and renal function in induced hyperuricemic rats.

The present study was conducted to evaluate the role of some natural safe source plants on the hyperuricemia in ratsusing Ginkgo biloba leaf extract and onion juice against hyperuricemia in induced hyperuricemic rats using potassium oxonate as an inducing agent. 40 Sprague Dawleystrain, mean weighting 80-110 g were used, albino rats were categorized into 4 equal groups as follows:Group GI: Control group: Only given basal diet, Group GII: Given basal diet plus 6.6 mg/kg/day plus potassium Oxonate. GIII: Co-treated group in which, rats were received basal diet, 6.6 mg/kg/day potassium oxonate plus gingko biloba leaf extract 80 mg/kg body weight. Group GIV: Co-treated group in which, rats were received basal diet, 6.6 mg/kg/day potassium Oxonate plus 10 mg/kg of onion At the end of 4 weeks, blood samples were collected and sera were separated and analyzed for determination of serum XO activities, UA, urea and creatinineconcentrations also the kidney specimens were removal for histopathological examination.

Authors

Els T Awad,SA Abdelaziz,Abdelbary Prince,IM Ahmed,Hussein A Elsayed

Published Date

2021/10/8

Influence of level of inclusion of Azolla leaf meal on growth performance, meat quality and skeletal muscle p70S6 kinase α abundance in broiler chickens

The interest in biodiesel production from oil-bearing seeds rather than soybean necessitates the scientific validation of other good quality protein sources that could substitute soybean meal in animal diets, particularly, broiler chickens where soybean meal constitutes a large portion of their diet. Therefore, the present study was conducted to investigate the effect of sun-dried Azolla leaf meal (ALM) as an unconventional dietary protein source in broiler chicken diet on growth performance, meat quality, skeletal muscle cell growth and protein synthesis through regulation of ribosomal protein S6 kinase (p70S6 kinase α). A total of 120 male Ross 308 broiler chicks were randomly allocated to three dietary treatments. Each treatment had four cages (i.e. replicates) with 10 birds/cage. The control group was fed with a corn–soy-based diet, the AZ5 group was supplemented with 5% ALM and the AZ10 group was …

Authors

AM Abdelatty,MI Mandouh,AK Al-Mokaddem,HA Mansour,HMA Khalil,AA Elolimy,H Ford,OAA Farid,A Prince,OG Sakr,SH Aljuaydi,M Bionaz

Journal

animal

Published Date

2020/11

In-depth hepatoprotective mechanistic study of Phyllanthus niruri: In vitro and in vivo studies and its chemical characterization

Phyllanthus niruri L. is a widespread tropical plant which is used in Ayurvedic system for liver and kidney ailments. The present study aims at specifying the most active hepatoprotective extract of P. niruri and applying a bio-guided protocol to identify the active compounds responsible for this effect. P. niruri aerial parts were extracted separately with water, 50%, 70% and 80% ethanol. The cytoprotective activity of the extracts was evaluated against CCl4-induced hepatotoxicity in clone-9 and Hepg2 cells. Bioassay-guided fractionation of the aqueous extract (AE) was accomplished for the isolation of the active compounds. Antioxidant activity was assessed using DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging method and ferric reducing antioxidant power (FRAP). The in vivo hepatoprotective activity of AE was evaluated in CCl4-induced hepatotoxicity in rats at different doses after determination of its LD50. Pretreatment of clone-9 and Hepg2 with different concentrations of AE (1, 0.1, 0.01 mg/ml) had significantly reduced the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) against CCl4 injures, and restored the activity of the natural antioxidants; glutathione (GSH) and superoxide dismutase (SOD) towards normalization. Fractionation of AE gave four fractions (I-IV). Fractions I, II, and IV showed a significant in vitro hepatoprotective activity. Purification of I, II and IV yielded seven compounds; corilagin C1, isocorilagin C2, brevifolin C3, quercetin C4, kaempferol rhamnoside C5, gallic acid C6, and brevifolin carboxylic acid C7. Compounds C1, C2, C5, and C7 showed the highest (p< 0.001) hepatoprotective …

Authors

Marwa I Ezzat,Mona M Okba,Sherif H Ahmed,Hossny A El-Banna,Abdelbary Prince,Shanaz O Mohamed,Shahira M Ezzat

Journal

PloS one

Published Date

2020/1/15

Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler …

Background Using probiotics have become popular. They are considered an alternative to Antibiotic Growth Promoters (AGP). Probiotics are supplemented into animal feed for improving growth performance along with preventing and controlling enteric pathogens. The aim of this work was to study the impact of dietary supplementation of Mannan-oligosaccharide and β-Glucan (Agrimos®) on broiler challenged with Escherichia coli O78 (E. coli O78 - marked with an antibiotic (320 μg ciprofloxacin/ml broth) on growth performance, serum biochemistry, immune organs-histopathology, E-coli colonization, and hepatic transcripts of Tumor necrosis factor-alpha (TNF-α) and Nuclear factor-kappa B (NF-ϰB). A total of 125 one-day-old chicks were used for conducting the experiment. Five one-day-old chicks were slaughtered for measuring the initial weight of the lymphoid tissue. The remaining chicks (120) were allotted …

Authors

Sabreen Ezzat Fadl,Ghada Ahmed El-Gammal,Osama Atia Sakr,Aly ABS Salah,Ayman Ali Atia,Abdelbary Mohammed Prince,Abdelhaleem Mohamed Hegazy

Journal

BMC veterinary research

Published Date

2020/12

Prevalence of multidrug resistance in the Egyptian methicillin-resistant Staphylococcus aureus isolates

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major health hazards and became of greater public health concern. This work aimed to shed substantial light on prevalence of MRSA in different clinical isolates and their resistance to different antibiotics. Among 258 Staphylococcus aureus isolates recovered from different clinical sources (urine, pus, throat swab, blood, seminal fluid, prostatic fluid, sputum swab, ascetic fluid, skin swab, nipple discharge and urinary catheter). 70 isolates were identified as MRSA. The highest percentage of MRSA was recorded from pus samples (57.1%) followed by urine (30%). Antimicrobial susceptibility test using 14 antibiotics showed that all MRSA were resistant to amoxicillin and cefuroxime, while only 50% were sensitive to vancomycin. High minimum inhibitory concentration of oxacillin (256 µg/ml) was detected in 12.9% of MRSA isolates.

Authors

Eman SH Ibrahim,Khaled El-Baghdady,Abd El-All,M Said,Mohamed AA Warda,Abdelbary M Prince,Mohamed Ibrahim

Journal

African Journal of Biological Sciences

Published Date

2020/12/1

Abdelbary Prince FAQs

What is Abdelbary Prince's h-index at Cairo University?

The h-index of Abdelbary Prince has been 12 since 2020 and 15 in total.

What are Abdelbary Prince's top articles?

The articles with the titles of

The puzzling etiologies of transient black discoloration in Nile Tilapia (Oreochromis niloticus) intensively cultured under RAS system

Efficiency of xylanase, emulsifier, and guanidinoacetic acid in restoring energy deficit in male broilers fed low metabolisable energy diets

Comparative Study between Mushroom-extracted and Commercial Lectins: Impact of Immune Response to H5N1, NDV, and IBD Vaccines in Broiler Chicken

Effects of phytase enzyme supplementation on growth performance, intestinal morphology and metabolism in Nile tilapia (Oreochromis niloticus)

Biochemical and Molecular Identification of the Most Common Streptococci Affecting Common pandora (Pagellus erythrinus Linnaeus, 1758) From the Mediterranean Coast of Tripoli

International Journal of Veterinary Science

Occurrence of toxigenic Clostridium difficile among diarrheic sheep and goats in rural settings: public health concern.

Impact of Anisakis pegreffi Infection on Gonadal Health and Gonadosomatic Index of European Hake (Merluccius merluccius)

...

are the top articles of Abdelbary Prince at Cairo University.

What are Abdelbary Prince's research interests?

The research interests of Abdelbary Prince are: Genomics and Proteomics

What is Abdelbary Prince's total number of citations?

Abdelbary Prince has 515 citations in total.

What are the co-authors of Abdelbary Prince?

The co-authors of Abdelbary Prince are Robert Linhardt, Jae Boum Youm, Mohamed A. Salem.

    Co-Authors

    H-index: 137
    Robert Linhardt

    Robert Linhardt

    Rensselaer Polytechnic Institute

    H-index: 29
    Jae Boum Youm

    Jae Boum Youm

    Inje University

    H-index: 17
    Mohamed A. Salem

    Mohamed A. Salem

    King Faisal University

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