Abdelaziz M. Hussein

Abdelaziz M. Hussein

Mansoura University

H-index: 22

Africa-Egypt

Abdelaziz M. Hussein Information

University

Mansoura University

Position

Professor of Medical Physiology Faculty of Medicine

Citations(all)

1501

Citations(since 2020)

1008

Cited By

873

hIndex(all)

22

hIndex(since 2020)

19

i10Index(all)

48

i10Index(since 2020)

38

Email

University Profile Page

Mansoura University

Abdelaziz M. Hussein Skills & Research Interests

Renal Physiology

Neurophysiology

Endocrine Physiology

Top articles of Abdelaziz M. Hussein

Effect of intermittent fasting on adriamycin-induced nephropathy: Possible underlying mechanisms

PurposeIntermittent fasting (IF) has been shown to induce a well-organized adaptive defense against stress inside the cells, which increases the production of anti-oxidant defenses, repair of DNA, biogenesis of mitochondria, and genes that combat inflammation. So, the goal of the current investigation was to identify the effects of IF on rats with adriamycin (ADR)-induced nephropathy and any potential underlying mechanisms.MethodsFour groups of 40 mature Sprague-Dawley male rats were allocated as follow; control, fasting, ADR, and ADR plus fasting. After 8 weeks of ADR administration urine, blood samples and kidneys were taken for assessment of serum creatinine (Cr), BUN, urinary proteins, indicators of oxidative damage (malondialdehyde (MDA), reduced glutathione (GSH) and Catalase (CAT) levels), histopathological examinations, immunohistochemical examinations for caspase-3, Sirt1, aquaporin2 …

Authors

Fathy H Elsaid,Abdelaziz M Hussein,Elsayed A Eid,Omar A Ammar,Ali Ali Khalil

Journal

Tissue and Cell

Published Date

2024/6/1

Human Wharton's jelly-derived mesenchymal stromal stem cells preconditioned with valproic acid promote cell migration and reduce renal inflammation in ischemia/reperfusion …

ObjectiveTo determine whether WJ-MSCs pretreated with VPA would enhance their migration to improve functional recovery of renal IRI in rats.Methods150 Sprague-Dawley rats were distributed into 5 groups; Sham, IRI, WJ-MSC, VPA, and WJ-MSCs + VPA. 10 rats were sacrificed after 3, 5, and 7 days. Role of WJ-MSCs pretreated with VPA was evaluated by assessment of renal function, antioxidant enzymes together with renal histopathological and immunohistopathological analyses and finally by molecular studies.ResultsWJ-MSCs and VPA significantly improved renal function and increased antioxidants compared to IRI group. Regarding gene expression, WJ-MSCs and VPA decreased BAX and TGF-β1, up-regulated Akt, PI3K, BCL2, SDF1α, and CXCR4 related to IRI. Additionally, WJ-MSCs pretreated with VPA improved the measured parameters more than either treatment alone.ConclusionWJ-MSCs …

Authors

Ahmed A Shokeir,Amira Awadalla,Eman T Hamam,Abdelaziz M Hussein,Mohamed R Mahdi,Alyaa Naeem Abosteta,Mirna Shahin,Nashwa Barakat,Mohamed El-Adl,Mohamed El-Sherbiny,Mamdouh Eldesoqui,Moneer AlMadani,Sahar K Ali,El-Said El-Sherbini,Salma M Khirallah

Journal

Archives of Biochemistry and Biophysics

Published Date

2024/4/4

LC/MS analysis of mushrooms provided new insights into dietary management of diabetes mellitus in rats

Mushrooms possess antihyperglycemic effect on diabetic individuals due to their nonfibrous and fibrous bioactive compounds. This study aimed to reveal the effect of different types of mushrooms on plasma glucose level and gut microbiota composition in diabetic individuals. The effects of five different mushroom species (Ganoderma lucidum, GLM; Pleurotus ostreatus, POM; Pleurotus citrinopileatus, PCM; Lentinus edodes, LEM; or Hypsizigus marmoreus, HMM) on alloxan‐induced diabetic rats were investigated in this study. The results indicated that LEM and HMM treatments showed lower plasma glucose levels. For the microbiota composition, ACE, Chao1, Shannon, and Simpson were significantly affected by PCM and LEM treatments (p < .05), while ACE, Shannon, and Simpson indexes were affected by HMM treatment (p < .01). Simpson index was affected in positive control (C+) and POM groups. All …

Authors

Mohammed Hamdy Farouk Abdelaziz Hussein,Abdallah Ghonimy,Hailong Jiang,Guixin Qin,Saeed El-Ashram,Saddam Hussein,Ibrahim Abd El-Razek,Tarek El-Afifi

Journal

Food Science & Nutrition

Published Date

2023/1/27

New Insight into the Possible Roles of L-Carnitine in a Rat Model of Multiple Sclerosis

Objective We investigated the effect of L-carnitine (LC) on cuprizone (Cup) demyelinating rat model and its possible underlying mechanisms. Methods Thirty male Sprague–Dawley (SD) rats were randomly allocated to three groups: the normal control group; the Cup group, in which Cup was administrated at a dose of 450 mg/kg per day orally via gastric gavage for 5 weeks; and the Cup + LC group, which received the same dose of Cup as the Cup group, except that the rats were treated additionally with LC 100 mg/kg/day orally for 5 weeks. The nerve conduction (NCV) in isolated sciatic nerves was measured; then, the sciatic nerves were isolated for H&E staining and electron microscope examination. The expression of myelin basic protein (MBP), IL-1β, p53, iNOS, and NF-KB by immunohistochemistry was detected in the isolated nerves. A PCR assay was also performed to detect the expression of antioxidant genes Nrf2 and HO-1. In addition, the level of IL-17 was measured by ELISA. Results There was a significant reduction in NCV in the Cup group compared to normal rats (p < 0.001), which was significantly improved in the LC group (p < 0.001). EM and histopathological examination revealed significant demyelination and deterioration of the sciatic nerve fibers, with significant improvement in the LC group. The level of IL-17 as well as the expression of IL-1β, p53, iNOS, and NF-KB were significantly increased, with significant reduction expression of MBP in the sciatic nerves (p < 0.01), and LC treatment significantly improved the studied parameters (p < 0.01). Conclusion The current study demonstrates a neuroprotective effect of LC in a Cup …

Authors

Sally M Safwat,Moutasem Salih Aboonq,Mahmoud El Tohamy,Moaz Mojaddidi,Saeed Awad M Al-Qahtani,Madaniah Omar Zakari,Ahmed A ElGendy,Abdelaziz M Hussein

Journal

Brain Sciences

Published Date

2023/12/25

Possible Role of Oxidative Stress and Nrf2/HO-1 Pathway in Pentylenetetrazole-induced Epilepsy in Aged Rats

Background:To examine the impact of aging on the response of rats to pentylenetetrazole (PTZ)-induction of epilepsy and the possible role of oxidative stress and nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase (HO)-1 pathway in this response.Methods:Forty male albino rats were equally allocated into 4 groups; 1) Young control (YC) group, aged 8-12 weeks, 2) Old control (OC) group, aged 24 months, 3) PTZ-Young group: young rats received PTZ (50 mg/Kg, ip every other day) for 2 weeks and 4) PTZ-Old group: as group 3 but rats were old. The seizure score stage and latency to the first jerk were recorded in rats. Redox state markers in brain tissues including malondialdehyde (MDA), catalase and total antioxidant capacity (TAC) were evaluated. Also, the expression of Nrf2 and HO-1 genes were measured in the brain tissues.Results:Old rats showed an early and a significant rise in the seizure …

Authors

Walaa Obydah,Ahmed Fathi Abouelnaga,Marwa Abass,Somaya Saad,Asmaa Yehia,Omar Abd-Alhakem Ammar,Alaa Mohamed Badawy,Mohie Mahmoud Ibrahim,Abdelaziz Mohamed Hussein

Journal

Reports of Biochemistry & Molecular Biology

Published Date

2023/4

Vanillic Acid Ameliorates Demyelination in a Cuprizone-Induced Multiple Sclerosis Rat Model: Possible Underlying Mechanisms

Objective To investigate the effect of vanillic acid (VA) on a Cuprizone (Cup) demyelinating rat model and the mechanisms behind such effect. Methods Thirty adult male Sprague Dawley (SD) rats were randomly divided into three groups: control, Cuprizone, and VA groups. Cuprizone was administrated at a dose of 450 mg/kg per day orally via gastric gavage for 5 weeks. The nerve conduction velocity (NCV) was studied in an isolated sciatic nerve, and then the sciatic nerve was isolated for histopathological examination, electron microscope examination, immunohistochemical staining, and biochemical and PCR assay. The level of IL17 was detected using ELISA, while the antioxidant genes Nrf2, HO-1 expression at the level of mRNA, expression of the myelin basic protein (MBP), interferon-gamma factor (INF)-γ and tumor necrosis factor (TNF)-α, and apoptotic marker (caspase-3) were measured using immunohistochemistry in the sciatic nerve. Results There was a significant reduction in NCV in Cup compared to normal rats (p < 0.001), which was markedly improved in the VA group (p < 0.001). EM and histopathological examination revealed significant demyelination and deterioration of the sciatic nerve fibers with significant improvement in the VA group. The level of IL17 as well as the expression of INF-γ and caspase-3 were significantly increased with a significant reduction in the expression of MBP, Nrf2, and HO-1 in the sciatic nerve (p < 0.01), and VA treatment significantly improved the studied parameters (p < 0.01). Conclusion The current study demonstrated a neuroprotective effect for VA against the Cup-induced demyelinating rat …

Authors

Sally M Safwat,Mahmoud El Tohamy,Moutasem Salih Aboonq,Amaal Alrehaili,Ahmad A Assinnari,Abdulrahman S Bahashwan,Ahmed A ElGendy,Abdelaziz M Hussein

Journal

Brain Sciences

Published Date

2023/12/22

Effect of melatonin on alpha synuclein and autophagy in dopaminergic neuronal differentiation of adipose mesenchymal stem cells

Background:The current work investigated the effect of melatonin on differentiation of adipose mesenchymal stem cells (AD-MSCs) into dopamine producing cells and its effect on autophagy process and alpha-Synuclein (α-Syn) secretion.Methods:AD-MSCs were characterized by flow cytometry and divided into 4 groups; i) control group (AD-MSCs without any treatment), ii) M+ MSCs group (MSCs treated with 1 µM melatonin for 12 days), iii) DN group (MSCs cultured in neurobasal A medium and essential neuronal growth factors for 12 days) and iv) DN+ M group (MSCs cultured in neurobasal A medium and 1µM melatonin for 12 days. By the end of experiments, the dopamine and α-Syn levels using ELISA, the expression of MAP-2, m-TOR and α-Syn genes at the level of mRNA and detection of autophagosomes formation using transmission electron microscope were performed.Results:We found that the isolated …

Authors

Hadeer Ahmed Mohamed Ibrahim,Abdelaziz Mohamed Hussein,Mahmoud Gabr,Rasha Aly El-Saeed,Omar Abd-Alhakem Ammar,Ahmed Abdulatif Hassan Mosa,Abdel-Aziz Fatouh Abdel-Aziz

Journal

Reports of Biochemistry & Molecular Biology

Published Date

2023/4

Impact of Cerium Oxide Nanoparticles on Metabolic, Apoptotic, Autophagic and Antioxidant Changes in Doxorubicin-Induced Cardiomyopathy: Possible Underlying Mechanisms

Background:In the current study, the effects of cerium oxide nanoparticles (nanocerium; NC) on doxorubicin (DOX)-induced cardiomyopathy and its possible underlying mechanisms were addressed.Methods:32 adult male rats were allocated into 4 groups; i) control group, ii) NC group; rats received NC (0.2 mg/kg, ip, daily), iii) DOX group; rats received DOX 4 mg/kg (2 injections with a 14-day interval), and iv) DOX+ NC group as DOX but rats received NC. At the end of the experiment, ECG and ECHO recordings and assessments of the levels of cardiac enzymes (CK-MB, LDH), and myocardial oxidative stress (MDA, catalase, and GSH), the expression of LC3 and beclin1 (markers of autophagy), caspase3 (marker of apoptosis) by immunohistochemistry, the expression of acetyl-CoA carboxylase alpha (ACCA) by PCR, and 5’adenosine monophosphate-activated protein kinase (AMPK) levels in the heart tissues …

Authors

Shorouk Elmorshdy Elsaeed Mohammed Elmorshdy,Gehan Ahmed Shaker,Zienab Helmy Eldken,Mahmoud Abdelbadie Salem,Amira Awadalla,Hany Mahmoud Abdel Shakour,Mohammed Elmahdy El Hosiny Sarhan,Abdelaziz Mohamed Hussein

Journal

Reports of Biochemistry & Molecular Biology

Published Date

2023/10

Possible Mechanisms of the Neuroprotective Actions of Date Palm Fruits Aqueous Extracts against Valproic Acid-Induced Autism in Rats

The current study aimed to determine how palm date aqueous fruit extracts (AFE) affected the autistic-like behaviors brought on by valproic acid (VPA) injection, as well as any potential contributions from Sirt-1, oxidative stress, apoptosis, and autophagy. The pregnant Sprague Dawley females were treated with VPA at 12.5th gestation day and pregnant females and their offspring were treated with AFE orally at doses of 4 mg/Kg by gastric gavage for 45 days after birth. The elevated plus-T maze, water maze, and rotarod tests were used to examine autism-like behaviors. At the end of the study, the expression of Nrf2, heme oxygenase (HO-1), Sirt-1, caspase-3 (a marker of apoptosis), LC3 (a marker of autophagy), and NFκB (inflammatory cytokines) were evaluated along with the oxidative stress in brain tissues and the histological changes in the cerebellum and hippocampus. The neurobehavioral assessments significantly declined due to VPA, which also significantly increased oxidative stress in the brain tissues and significantly decreased Nrf2 and HO-1 expression. Additionally, VPA administration caused significant increase in the expression of caspase-3 in the cerebellar cortex, not in the hippocampus; LC3 and NFκB in the hippocampus, not in the cerebellar cortex; and significant reduction in the expression of Sirt-1 in the hippocampus, not in the cerebellum. On the other hand, AFE treatment significantly improved the neurobehavioral changes as well as it improved significantly the oxidative stress and the expression of LC3, NFκB, NrF2, HO-1, and Sirt-1 in the cerebellum and hippocampus. Conclusions: AFE administration might improve the …

Authors

Abdelaziz M Hussein,Seham Ahmed Mahmoud,Khalid Mohammed Elazab,Ahmed F Abouelnaga,Marwa Abass,Ahmed AH Mosa,Mennatullah AM Hussein,Mohamed EG Elsayed

Journal

Current Issues in Molecular Biology

Published Date

2023/2/14

Effect of melatonin on oxidative stress of differentiated Dopaminergic cells

BackgroundAlthough stem cells therapies provide a great deal in the treatment of several disease, they lack their normal functions after transplantation due to inflammation and oxidative stress. Melatonin has a powerful antioxidant ability and can enhance the effect of stem cells.ObjectivesThis work aimed to investigate the melatonin’s effect on oxidative stress of differentiated adipose-mesenchymal stem cells (AD-MSCs) to dopaminergic (DAergic) cells.

Authors

Hadeer AM Ibrahim,Abdelaziz M Hussein,Mahmoud Gabr,AF Abdel–Aziz,Rasha A El-Saeed

Journal

Egyptian Pharmaceutical Journal

Published Date

2023/7/1

Proline hydroxylase 2 (PHD2) promotes brown adipose thermogenesis by enhancing the hydroxylation of UCP1

ObjectiveBrown adipose tissue (BAT) plays a crucial role in regulating non-shivering thermogenesis under cold exposure. Proline hydroxylases (PHDs) were found to be involved in adipocyte differentiation and lipid deposition. However, the effects of PHDs on regulatory mechanisms of BAT thermogenesis are not fully understood.MethodsWe detected the expression of PHDs in different adipose tissues by using immunoblotting and real-time PCR. Further, immunoblotting, real-time PCR, and immunostaining were performed to determine the correlation between proline hydroxylase 2 (PHD2) and UCP1 expression. Inhibitor of PHDs and PHD2-sgRNA viruses were used to construct the PHD2-deficiency model in vivo and in vitro to investigate the impacts of PHD2 on BAT thermogenesis. Afterward, the interaction between UCP1 and PHD2 and the hydroxylation modification level of UCP1 were verified by Co-IP …

Authors

Fan Li,Fenglin Zhang,Xin Yi,Lu Lu Quan,Xiaohua Yang,Cong Yin,Zewei Ma,Ruifan Wu,Weijie Zhao,Mingfa Ling,Limin Lang,Abdelaziz Hussein,Shengchun Feng,Yiming Fu,Junfeng Wang,Shuyi Liang,Canjun Zhu,Lina Wang,Xiaotong Zhu,Ping Gao,Qianyun Xi,Yongliang Zhang,Lin Zhang,Gang Shu,Qingyan Jiang,Songbo Wang

Journal

Molecular Metabolism

Published Date

2023/7/1

Retinoic acid potentiates the therapeutic efficiency of bone marrow-derived mesenchymal stem cells (BM-MSCs) against cisplatin-induced hepatotoxicity in rats

(1) Background: Hepatotoxicity is a common health problem, and oxidative stress plays a crucial role in its underlying mechanisms. We inspected the possible effect of retinoic acid (RA) in the potentiation of hepatoprotective effect of bone marrow mesenchymal stem cells (BM-MSCs) against Cisplatin (Cis)-induced hepatotoxicity. (2) Methods: 60 male Sprague Dawley rats (SD) were separated randomly and designated to six main equal groups as follows: (1) Control group, (2) Cis group (rats got Cis 7 mg/Kg i.p.), (3) Cis + vehicle group (as group 2, but rats received the (vehicle) culture media of BM-MSCs), (4) Cis as in group 2 + BM-MSCs (1x106), (5) Cis as for group 2 + RA 1 mg/Kg i.p., and (6) Cis and BM-MSCs as for group 3 + RA as for group 4. Liver injury was assessed by measuring liver enzymes (ALT, AST), while liver toxicity was evaluated by histopathological examination. Apoptotic marker caspase-3 protein was detected immunohistochemically. Real time PCR was performed to detect NADPH oxidase and TNF-α at transcription levels. Oxidative stress was investigated by colorimetric measurement of MDA, GSH and catalase. (3) Results: Contrary to the Cis group (p < 0.05), BM-MSCs/RA supplementation resulted in a substantial decrease in serum levels of hepatic impairment indicators such as ALT, AST and oxidative stress markers such as MDA, as well as an increase in hepatic GSH, Catalase, and a decrease in expression of TNF-α and downregulation of NADPH oxidase. The improvement after therapy with BM-MSCs/RA was confirmed by histopathological examination. Moreover, the downregulation of caspase-3 in liver tissue …

Authors

Maha M Azzam,Abdelaziz M Hussein,Basma H Marghani,Nashwa M Barakat,Mohsen MM Khedr,Nabil Abu Heakel

Journal

Scientia pharmaceutica

Published Date

2022/9/29

Effect of vanillic acid on pentylenetetrazole-kindled rats: Nrf2/HO-1, IGF-1 signaling pathways cross talk

Vanillic acid (VA) exhibited antioxidant and neuroprotective properties in some neurodegenerative disorders. So, the current study examined the neuroprotective potential of VA as an antiepileptic agent in pentylenetetrazole (PTZ)-induced epileptic rats and the prospective role of Insulin like growth factor-1 (IGF-1) and nuclear factor-2 erythroid-related factor-2 (Nrf2)/heme oxygenase-1 (HO-1) pathway in this respect. Thirty male albino rats were equally subdivided into 3 groups; (1) normal control (NC) group, (2) PTZ-group: received PTZ (50 mg/Kg, i.p. every other day) for 14 days, and (3) PTZ + VA group: received PTZ and VA (50 mg/Kg daily for 2 weeks). The seizure score and latency were evaluated after PTZ injection. Also, the markers of oxidative stress (malondialdehyde (MDA), catalase, and reduced glutathione (GSH)), histopathological examination, the expression of glial fibrillary acidic protein (GFAP) (a marker of astrocytes) IGF-1, Nrf2, and HO-1 were assessed in the brain tissues by the end of the experiment. PTZ caused significant decrease in seizure latency and significant increase in seizure score by the end of the experiment (p < 0.01). This was associated with significant increase in MDA and GFAP with significant decrease in GSH, total antioxidant capacity (TAC) and IGF-1 in brain tissues compared to normal group (p < 0.01). On the other hand, treatment with VA caused significant attenuation in PTZ-induced seizures which was associated with significant improvement in oxidative stress markers and downregulation in GFAP and upregulation of Nrf2, HO-1 and IGF-1 in CA3 hippocampal region (p < 0.01). VA showed …

Authors

Mahmoud A El-Hefnawy,Asmaa Yehia,Eman M El Nashar,Somaya Saad,Walaa Obydah,Mansour A Alghamdi,Wardah A Alasmari,Abdelaziz M Hussein

Journal

Journal of Integrative Neuroscience

Published Date

2022/1/28

Ceftriaxone as a novel therapeutic agent for hyperglutamatergic states: Bridging the gap between preclinical results and clinical translation

Dysregulation of glutamate homeostasis is a well-established core feature of neuropsychiatric disorders. Extracellular glutamate concentration is regulated by glutamate transporter 1 (GLT-1). The discovery of a beta-lactam antibiotic, ceftriaxone (CEF), as a safe compound with unique ability to upregulate GLT-1 sparked the interest in testing its efficacy as a novel therapeutic agent in animal models of neuropsychiatric disorders with hyperglutamatergic states. Indeed, more than 100 preclinical studies have shown the efficacy of CEF in attenuating the behavioral manifestations of various hyperglutamatergic brain disorders such as ischemic stroke, amyotrophic lateral sclerosis (ALS), seizure, Huntington’s disease, and various aspects of drug use disorders. However, despite rich and promising preclinical data, only one large-scale clinical trial testing the efficacy of CEF in patients with ALS is reported. Unfortunately, in that study, there was no significant difference in survival between placebo- and CEF-treated patients. In this review, we discussed the translational potential of preclinical efficacy of CEF based on four different parameters: (1) initiation of CEF treatment in relation to induction of the hyperglutamatergic state, (2) onset of response in preclinical models in relation to onset of GLT-1 upregulation, (3) mechanisms of action of CEF on GLT-1 expression and function, and (4) non-GLT-1-mediated mechanisms for CEF. Our detailed review of the literature brings new insights into underlying molecular mechanisms correlating the preclinical efficacy of CEF. We concluded here that CEF may be clinically effective in selected cases in acute and …

Authors

Osama A Abulseoud,Fawaz Alasmari,Abdelaziz M Hussein,Youssef Sari

Published Date

2022/7/5

Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism

Objectives The current work was designed to study the effect of dehydroepiandrosterone (DHEA) on glucose homeostasis, liver functions and hemostatic disturbances in a rat model of bilateral orchidectomy (ORCH). Methods 32 male rats (n = 8) were randomly assigned into 4 groups; (i) control (sham operated) group; were normal rats in which all surgical procedures were done without ORCH, (ii) Control + DHEA group: as control group but rats were treated with DHEA for 12 weeks, (iii) orchiectomized (ORCH) group: rats had bilateral orchidectomy and (iv) ORCH + DHEA group: orchiectomized rats treated with DHEA for 12 weeks. Four weeks after ORCH, DHEA treatment began and lasted for twelve weeks. By the end of the experiment, the parameters of glucose homeostasis, lipid profile, liver enzymes, bleeding and clotting times (B.T. and C.T.), prothrombin time (P.T.), activated partial thromboplastin time (aPTT), platelet count and aggregation, von-Willebrand factor (vWF), fibrinogen, plasminogen activator inhibitor (PAI-1), fibrin degradation products (FDP), intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), endothelin-1 were measured. Results ORCH caused significant deteriorations in the parameters of glucose homeostasis, lipid profile, and liver functions (p < 0.05). In addition, lower androgenicity-induced by ORCH caused a significant rise in PAI-1, fibrinogen, FDPs, ET-1 (p < 0.01) with significant shortening of bleeding and clotting times. DHEA replacement therapy significantly decreased glucose, insulin, PAI-1, fibrinogen, ICAM-1, and VCAM-1 when compared to ORCH rats. Conclusion DHEA …

Authors

Sally M Safwat,Abdelaziz M Hussein,Elsayed A Eid,Mohamed S Serria,Basem H Elesawy,Hussein F Sakr

Journal

Scientia Pharmaceutica

Published Date

2022/1/20

Possible underlying mechanisms for the renoprotective effect of retinoic acid-pretreated wharton’s jelly mesenchymal stem cells against renal ischemia/reperfusion Injury

Objectives The current work investigated the effect of Wharton jelly mesenchymal stem cells (WJ-MSCs) pretreated with all-trans-retinoic acid (ATRA) on renal ischemia in rats and the possible role of oxidative stress, apoptotic and Wnt/β-Catenin signaling pathways, and inflammatory cytokines in their effects. Methods The study included 90 male Sprague Dawley rats that were allocated to five groups (n = 18 rats): (I) Sham-operated group (right nephrectomy was performed); (II) Ischemia/reperfusion injury (IRI) group, a sham group with 45-min renal ischemia on the left kidney; (III) ATRA group, an ischemic group with an intravenous (i.v.) administration of ATRA 10 µM, 10 min post-surgery); (IV) WJ-MSCs group, an IRI group with an i.v. administration of 150 µL containing 7 × 106 WJ-MSCs, 10 min post-surgery; (V) WJ-MSCs + ATRA group, an IRI group with an i.v. administration of 150 µL of 7 × 106 WJ-MSCs pretreated with 10 µM ATRA. At the end of the experiments, serum creatinine, BUN micro-albuminuria (MAU), urinary protein, markers of redox state in the left kidney (MDA, CAT, SOD, and GSH), and the expression of Bax, IL-6, HIF-1α, Wnt7B, and β-catenin genes at the level of mRNA as well as for immunohistochemistry for NFkB and β-Catenin markers were analyzed. Results The current study found that 45-min of renal ischemia resulted in significant impairment of kidney function (evidenced by the increase in serum creatinine, BUN, and urinary proteins) and deterioration of the kidney morphology, which was associated with a significant increase in redox state (evidenced by an increase in MDA and a decrease in GSH, SOD, and CAT), and …

Authors

Mai Barakat,Abdelaziz M Hussein,Mohamed F Salama,Amira Awadalla,Nashwa Barakat,Mohamed Serria,Mohamed El-Shafey,Mohamed El-Sherbiny,Mohamed A El Adl

Journal

Cells

Published Date

2022/6/22

N. Sayed O, S Said E, Mwaheb MA. Spirulina Ameliorates Oxidative Damage and Inflammation in Rotenone-Induced Neurotoxicity in Male Mice

Background: Rotenone, a chemical compound produced naturally by leguminous plants, has conventionally been used as a pesticide by blocking the uptake of oxygen by body cells. Our study aimed to investigate the effect of spirulina on oxidative damage, inflammation, and neurotoxicity in male mice treated by rotenone.Methods: The experimental animals were divided into 5 groups. Group (I) served as control that received Dimethyl Sulfoxide (DMSO); Group (II) mice treated with rotenone (1.5 mg/kg, sc 3 times per week); Group (III) mice received rotenone/L-dopa (25 mg/kg, PO daily); Group (IV) and Group (V) mice were treated with rotenone/spirulina (200 and 400 mg/kg, PO daily) respectively for two weeks.Results: Rotenone-treated mice indicated impaired motor coordination and activity in wire hanging, wood walking, open field, and stair tests. Furthermore, rotenone treatment caused elevation in striatal levels of Malondialdehyde (MDA), Nitric Oxide (NO), Tumor Necrosis Factor (TNF-α), Interleukin-1 beta (IL-1β), and caspase 3 and decrement in Bcl-2; dopamine and Glutathione (GSH) levels. Moreover, severe neuronal degeneration, striatal DNA fragmentation, and increased striatal 8-OHdG levels and MTH1 expression in the rotenone group. Additionally, spirulina treatment prevented rotenone-induced motor deficits striatal DNA fragmentation and demonstrated good restoration of the substantial neurons with reservation of the typical dark appearance. Besides, rotenone-induced biochemical changes were ameliorated by spirulina treatment as dopamine, Bcl-2, and GSH levels were increased, and striatal MDA, TNF-α, IL-1β, and caspase …

Authors

MEA El-Shamarka,AMS Hussein

Journal

International Journal of Medical Toxicology and Forensic Medicine

Published Date

2022

Rapamycin improves adipose-derived mesenchymal stem cells (ADMSCs) Renoprotective effect against cisplatin-induced acute nephrotoxicity in rats by inhibiting the mTOR/AKT …

Objective Because the poor survival of transplanted cells in a hostile microenvironment limits stem cell therapy, in the current study, we investigated the effect of rapamycin (Rapa)-preactivated autophagy on the survival and homing of transplanted adipose mesenchymal stem cells (ADMSCs) in a rat model of cisplatin (Cis)-induced nephrotoxicity, as well as the possible role of the mTOR/AKT signaling pathway. Materials and methods In vitro, ADMSCs isolated from rats were treated with 50 nmol/L rapamycin for 2 h, after which the cytoprotective and autophagy-inducing effects of Rapa were investigated. The cis-induced acute nephrotoxicity rat model was constructed in vivo. ADMSCs and Rapa-ADMSCs were administered into the tail vein before Cis therapy. At 3, 7, and 10 days after Cis injection, all animals were euthanized. The renal functions and morphology as well as autophagy response were assessed. Results The pretreatment of cultured ADMSCs with Rapa caused a significant increase in autophagic activities and lysosome production of the cells, with a significant increase in the secretion of SDF-1, IL-10 and autophagy promoter LC3 and Beclin from these cells, while mTOR/AKT pathways were inhibited. In addition, the transplantation of Rapa-pretreated ADMSCs restored the kidney functions and morphology dramatically. Renal expression of SDF-1 and HIF1 was upregulated, while expression of IL-6, NF-kB and TGF-β1 was downregulated. Conclusions We concluded that the preactivation of autophagy with Rapa improves the survival and differentiation of the transplanted ADMSCs by inhibiting the mTOR/AKT signaling pathway …

Authors

Amira Awadalla,Abdelaziz M Hussein,Yousra M El-Far,Fardous F El-Senduny,Nashwa Barakat,Eman T Hamam,Hanaa M Abdeen,Mohamed El-Sherbiny,Mohamed S Serria,Amira A Sarhan,Asmaa M Sena,Ahmed A Shokeir

Journal

Biomedicines

Published Date

2022/5/31

Neck Ultrasound Can Be Helpful in Diagnosing and Assessing Severity of OSA

Neck Ultrasound Can be Helpful in Diagnosing and Assessing Severity of OSA a Abstract Objective: The purpose of this study was to assess the role of submental ultrasonography (US) as a useful bedside tool for diagnosing obstructive sleep apnea (OSA) and to determine whether a link existed between US findings and OSA severity. Subjects and methods:: This observational casecontrol study was conducted in 90 subjects (45 patients with confirmed polysomnographic OSA, 23 obese participants and 22 non-obese participants) with a complete history, clinical examination, Routine laboratory and pulmonary function tests were performed for all subjects. Neck US was done also for all participants by an independent operator who was blinded to the PSG results. Results: distance between 2 lingual arteries (DLA) was significantly increased among OSA patients vs. control obese and non-obese groups (38.3±6.6 vs …

Authors

A Hussein,RA Sedeek,M Higazi,AO Abdelaziz

Published Date

2022/5

Zinc oxide nanoparticles and spironolactone-enhanced Nrf2/HO-1 pathway and inhibited Wnt/β-catenin pathway in adenine-induced nephrotoxicity in rats

ObjectiveTo investigate the renoprotective, the antioxidant, and the anti-inflammatory impact of a combination of SPL and ZnO-NPs to combat against chronic kidney disease (CKD).MethodsIn total, 50 males of rats were distributed into 5 groups (10 rats each); normal group, adenine sulfate (0.25% in diet for 10 days) (CKD) group. After the last dose of adenine sulfate, rats were divided into three groups: SPL + Adenine sulfate group; rats were treated orally by mixing SPL (20 mg/kg/day) into chow for 8 weeks, ZnO-NPs + Adenine sulfate group; rats were injected intraperitoneally with ZnO-NPs (5 mg/kg) three times weekly for 8 weeks, ZnO-NPs + SPL + Adenine sulfate group; rats were injected with the same previous doses for 8 weeks.ResultsEach of SPL and ZnO-NPs up-regulated antioxidant genes (Nrf2 and HO-1), down-regulated fibrotic and inflammatory genes (TGF-β1, Wnt7a, β-catenin, fibronectin …

Authors

Amira Awadalla,Eman T Hamam,Fardous F El-Senduny,Nisreen Mansour Omar,Mohamed R Mahdi,Nashwa Barakat,Omar A Ammar,Abdelaziz M Hussein,Ahmed A Shokeir,Salma M Khirallah

Journal

Redox Report

Published Date

2022/12/31

Effects of Stevia rebaudiana Bertoni extracts in the rat model of epilepsy induced by pentylenetetrazol: Sirt-1, at the crossroads between inflammation and apoptosis

The current study investigated the effects of stevia extracts on a PTZ-induced epileptic rat model and its potential mechanism. Thirty male Sprague-Dawley rats were equally subdivided into 3 groups; (1) normal control (NC) group, (2) PTZ-group: received PTZ (50 mg/kg, i.p. every other day) for 2 weeks, and (3) PTZ+ Stevia group: received PTZ and stevia (200 mg/kg orally daily) for 4 weeks (2 weeks before the start of PTZ treatment and 2 weeks with PTZ administration). The first jerk latency and the seizure score were assessed in rats. Also, brain tissue samples were collected by the end of the experiment, and oxidative stress markers (catalase, MDA, and total antioxidant capacity (TAC)) were measured by biochemical analysis in hippocampal brain homogenates. Also, in the hippocampus, the expression of IL6 and Bcl-2 at the mRNA level and expression of Sirt-1, P53, caspase-3, GFAP, and NF-kB in CA3 hippocampal region by immunohistochemistry was investigated. PTZ substantially increased the seizure score and decreased the seizure latency. Also, PTZ significantly increased MDA, GFAP, IL-6, NF-kB, caspase-3, and p53 and significantly reduced Sirt-1, TAC, and Bcl-2 in hippocampal tissues compared to the control group (p < 0.01). However, Stevia Rebaudiana Bertoni (Stevia R.) significantly attenuated the PTZ-induced seizures, improved oxidative stress markers, downregulated GFAP, IL-6, NF-kB, caspase-3, and p53, and upregulated Sirt-1 and Bcl-2 in the CA3 hippocampal region (p < 0.01). In conclusion, Stevia R. exhibits neuroprotective and antiepileptic actions in PTZ-induced epilepsy due to its antioxidant, anti …

Authors

Eman M El Nashar,Walaa Obydah,Mansour A Alghamdi,Somaya Saad,Asmaa Yehia,Ayda Maryoud,Nedaa A Kiwan,Wardah A Alasmari,Abdelaziz M Hussein

Journal

Journal of Integrative Neuroscience

Published Date

2022/1/28

The role effects of dietary fiber on intestinal microbial composition and digestive physiological functions of pigs: A review

The intestinal microbes do not only provide nutrients for their host, but it also contributes to the maintenance of intestinal micro ecological balance, which are essential for ensuring a healthy development of the intestine, dietary fiber is one of the substrates for intestinal microbial fermentation. This can improve the structure of intestinal microflora and is of great significance in maintaining intestinal health. The research on the efficacy of dietary fiber on pig intestinal microbes and the factors affecting the intestinal microbial utilization of dietary fibre in pigs is reviewed, which provides a theoretical basis for further research on the production practice of dietary fiber addition in pig breeding industry. As well, a good digestive physiological environment is essential for pigs to benefit from the nutrients and to increase their productivity. Adding the appropriate amounts of fiber to pig diets plays an important role in regulating the …

Authors

Saddam Hussein,Yu Xiaoying,Mohammed Hamdy Farouk,Ahmed Abdeen,Abdelaziz Hussein,Jiang Hailong

Published Date

2021

Investigation on the Effects of GLP1 analog and SGLT2 inhibitor against Diabetic Cardiomyopathy in Type 2 Diabetic Rats: Possible Underlying Mechanisms

Background The present study investigated the possible cardioprotective effects of GLP1 and SGLT2i against diabetic cardiomyopathy (DCM) in type 2 diabetic rats and the possible underlying mechanisms. DCM is defined as the presence of heart failure in absence of coronary artery disease. Methods Thirty-two male Sprague Dawley rats were randomly subdivided into 4 equal groups; a) control group, b) DM group, type 2 diabetic rats with saline daily for 4 weeks, c) DM+ GLP1, as DM group with GLP1 analogue (liraglutide) at a dose of 75 µg/kg for 4 weeks, and d) DM+ SGLT2i: as DM group with SGLT2 inhibitor (dapagliflozin) at a dose of 1mg/kg for 4 weeks. By the end of treatment (4 weeks), serum blood glucose, HOMA-IR, insulin and cardiac enzymes (LDH, CK-MB) were measured. Also, the cardiac histopathology, myocardial oxidative stress markers (MDA, GSH and CAT) and norepinephrine (NE …

Authors

Abdelaziz M Hussein,Elsayed A Eid,Medhat Taha,Rami M Elshazli,Raouf Fekry Bedir,Lashin Saad Lashin

Published Date

2021/5/10

MP52-02 LONG-TERM EFFECT OF LOCAL AND SYSTEMIC ADIPOSE TISSUE-DERIVED STEM CELLS ON STRESS URINARY INCONTINENCE IN RATS

INTRODUCTION AND OBJECTIVEStress urinary incontinence (SUI) is a major health problem that has many social and economic impacts, so we evaluated the long-term effect of locally and systemically administrated adipose tissue-derived mesenchymal stem cells (ADMSCs) on the treatment of SUI-induced in a rat model.METHODSThirty-two female Sprague-Dawley rats were randomly allocated into 4 groups (8 rats each); control, SUI, local ADMSCs and systemic ADMSCs groups. SUI was induced by vaginal dilatation of the rats via inflating the balloon of a urethral catheter with 5 mL sterile saline in the vagina for 8 hours. Rats then received 0.9% saline, local ADMSCs treatment (periurethal injection at 5 and 7 o’clock positions of the vesico-urethral junction) and systemic ADMSCs treatment (injection in the tail vein) in SUI, local and systemic ADMSCs groups, respectively for 4 weeks. Urodynamic study in terms …

Authors

Arwa Elsharabasy,Ahmed Elkashef,Nashwa Barakat,Sherry Khater,Rawdy Ashour,Amira Awadalla,Abdelaziz Hussein,Mohamed Gomaa,Mahmoud Abdel-Maboud,Ahmed Shokeir

Journal

The Journal of Urology

Published Date

2021/9

Safety and tolerability of sodium glucose co-transporter-2 inhibitors (SGLT2i) during Ramadan fasting

Background: Diabetic management during Ramadan fasting is a significant clinical challenge. Sodium glucose co-transporter-2 inhibitors (SGLT2i) are new class of antidiabetic medications known for low frequency of associated hypoglycemia. The present study aimed to evaluate efficacy, safety and tolerability of SGLT2i in diabetic patients practicing Ramadan fasting. Patients and methods: The study included 94 patients. They comprised 51 patients who received metformin and sulfonylureas (SU): glimepiride (1-6 mg/d) or gliclazide MR (60-120mg/d) and 43 patients who received metformin and SGLT2i: empagliflozin (25 mg), dapagliflozin (10 mg) or canagliflozin (300 mg). The study outcome parameters were frequency of hypoglycemia episodes, volume depletion episodes, number of days with early breaking of fasting and missed fasting days. Results: It was found that patients in SGLTi group experiences significantly fewer symptomatic (9.3% versus 35.3%, p= 0.003) and documented (7.0% versus 25.5%, p= 0.017) hypoglycemic episodes as compared to the SU group. However, there were no significant differences between the studied groups regarding the frequency of patients with volume depletion episodes (5.9% versus 16.3%, p= 0.1). Moreover, there were no significant differences between groups regarding the frequency of patients with early breaking of fasting (11.8% versus 9.3%, p= 0.7) or missed fasting (3.9% versus 2.3%, p= 0.66). None of the studied patients discontinued the prescribed medications. Conclusions: SGLT2i combined with metformin for diabetic patients during Ramadan fasting are effective, safe and well-tolerated …

Authors

Elsayed A Eid,Abdelaziz M Hussein,Hossam A Ghazy

Journal

Clinical Diabetology

Published Date

2021

Possible mechanisms for the renoprotective action of adipose-derived mesenchymal stem cells with CD44-targeted hyaluronic acid against renal ischemia

The present study aimed to investigate the invitro preconditioning of adipose-derived mesenchymal stem cells (ADMSCs) with CD44-targeted hyalournic acid (HA) on ischemic kidney injury in rats. Ninety male Sprague Dawley rats were randomly allocated into the following groups; i) sham group, ii) control group: rats exposed to 45 min left renal ischemia with saline treatment, iii) HA group as control group but rats treated with HA, iv) ADMSCs group as control but rats treated with ADMSCs v) HA + ADMSCs group as ADMSCs but rats treated with ADMSCs preconditioned with CD44-tageted HA for 14 days. We found that treattment with either ADMSCs or HA + ADMSCs caused significant decrease in the elevated serum creatinine and BUN and malondialdehyde (MDA) concentrations and expression of TGF-β1, fibronectin, collagen type I, inducible nitric oxide synthease (iNOS) and microRNAs (miR-21, miR-17-5p …

Authors

Amira Awadalla,Abdelaziz M Hussein,Mohamed Ali,Nashwa Barakat,Eman T Hamam,Raghda W Magar,Ahmed A Shokeir

Journal

Life Sciences

Published Date

2021/5/1

MP52-01 SHORT-TERM EFFECT OF LOCAL AND SYSTEMIC ADIPOSE TISSUE-DERIVED STEM CELLS ON STRESS URINARY INCONTINENCE IN RATS

INTRODUCTION AND OBJECTIVEStress urinary incontinence (SUI) is a major health problem that has many social and economic impacts, so we evaluated the short-term effect of locally and systemically administrated adipose tissue-derived mesenchymal stem cells (ADMSCs) on the treatment of SUI-induced in a rat model.METHODSThirty-two female Sprague-Dawley rats were randomly allocated into 4 groups (8 rats each); control, SUI, local ADMSCs and systemic ADMSCs groups. SUI was induced by vaginal dilatation of the rats via inflating the balloon of a urethral catheter with 5 mL sterile saline in the vagina for 8 hours. Rats then received 0.9% saline, local ADMSCs treatment (periurethal injection at 5 and 7 o’clock positions of the vesico-urethral junction) and systemic ADMSCs treatment (injection in the tail vein) in SUI, local and systemic ADMSCs groups, respectively for 2 weeks. Urodynamic study in terms …

Authors

Arwa Elsharabasy,Ahmed Elkashef,Nashwa Barakat,Sherry Khater,Amira Awadalla,Abdelaziz Hussein,Mohamed Gomaa,Mahmoud Abdel-Maboud,Ahmed Shokeir

Journal

The Journal of Urology

Published Date

2021/9

PHD3 mediates denervation skeletal muscle atrophy through Nf‐κB signal pathway

Skeletal muscle is the largest organ of the body, the development of skeletal muscle is very important for the health of the animal body. Prolyl hydroxylases (PHDs) are the classical regulator of the hypoxia inducible factor (HIF) signal pathway, many researchers found that PHDs are involved in the muscle fiber type transformation, muscle regeneration, and myocyte differentiation. However, whether PHDs can impact the protein turnover of skeletal muscle is poorly understood. In this study, we constructed denervated muscle atrophy mouse model and found PHD3 was highly expressed in the atrophic muscles and there was a significant correlation between the expression level of PHD3 and skeletal muscle weight which was distinct from PHD1 and PHD2. Then, the similar results were getting from the different weight muscles of normal mice. To further verify the relationship between PHD3 and skeletal muscle protein …

Authors

Fan Li,Cong Yin,Zewei Ma,Kelin Yang,Lijuan Sun,Chen Duan,Tao Wang,Abdelaziz Hussein,Lina Wang,Xiaotong Zhu,Ping Gao,Qianyun Xi,Yongliang Zhang,Gang Shu,Songbo Wang,Qingyan Jiang

Journal

The FASEB Journal

Published Date

2021/4

Effect of zinc oxide nanoparticles and ferulic acid on renal ischemia/reperfusion injury: possible underlying mechanisms

ObjectivesThe present study examined the effects of ferulic acid (FA) and Zinc oxide nanoparticles (ZnO-NPs) and a combination of both on renal ischemia/reperfusion injury (IRI) in rats and their possible underlying mechanisms.Methodstwo-hundreds male Sprague Dawley rats were randomly allocated into the 5 groups; i) sham group, ii) control (IRI) group (occlusion of the left renal pedicle for 45 min), iii) FA group as IRI group with FA (100 mg/Kg oral 24 hrs before ischemia), iv) ZnO-NPs group as IRI group with ZnO-NPs single 5 mg/Kg i.p. 2 hrs before ischemia and v) FA + ZnO-NPs group as IRI group with both FA and ZnO-NPs in the same previous doses. According to the reperfusion times, each group was further subdivided into 4 hr, 24 hr, 48 hr and 7 days reperfusion subgroups.Resultsadministration of either FA or ZnO-NPs caused significant improvement in the elevated serum creatinine and BUN and …

Authors

Amira Awadalla,Abdelaziz M Hussein,M Yousra,Nashwa Barakat,Eman T Hamam,Mohamed El-Sherbiny,Mohamed El-Shafey,Ahmed A Shokeir

Journal

Biomedicine & Pharmacotherapy

Published Date

2021/8/1

Smart stadium using cloud computing and Internet of Things (IoT): Existing and new models

In our life technology is important there are two entirely various technologies are cloud computing and the IoT and both are very portion of our lives. They are supposed to be more common in their acceptance and use, making them essential ingredients of the future Internet worldwide. Because of the lack of time in our working life and the follow-up of all operations that we must follow before any match is held on any stadium in the world. An aspect of precautionary measures is discussed here before every match. In this research, a discussion was conducted on how to integrate cloud computing and the IoT and use them to work in developing stadiums in the word and made it smart. Several existing and new models of smart stadium are although explained.

Authors

Mustafa Jamal Mahdi,Abbas Fadhil Aljuboori,AM Hussein

Journal

International Journal of Computer Applications Technology and Research

Published Date

2021

Chronic valproic acid administration enhances oxidative stress, upregulates IL6 and downregulates Nrf2, Glut1 and Glut4 in rat’s liver and brain

Valproic acid (VPA) is a powerful antiepileptic drug that was associated with several neurological and hepatic problems especially with increasing its dose and duration. These problems may be metabolic in origin and related to glucose homeostasis. So, the present study investigated the effect of different doses and durations of VPA on the expression of glucose transporters (Glut1 and Glut4), oxidative stress and inflammatory cytokine (IL-6) in the liver and specific brain regions. Seventy-two male Sprague–Dawley rats were randomly allocated into three equal groups:(1) saline group,(2) 200 mg VPA group and (3) 400 mg VPA group. By the end of experiments, the expressions of Glut1, Glut4 nuclear factor erythroid-like 2 related factor (Nrf2), IL-6 and oxidative stress markers [malondialdehyde (MDA) and glutathione (GSH)] in the liver, corpus striatum, prefrontal cortex (PFC) and cerebellum were assessed. We …

Authors

Abdelaziz M Hussein,Amira Awadalla,Khaled M Abbas,Hussein F Sakr,Rasha Elghaba,Gamal Othman,Naglaa Mokhtar,Ghada M Helal

Journal

NeuroReport

Published Date

2021/7/7

Effect of intermittent fasting on glucose homeostasis and bone remodeling in glucocorticoid-induced osteoporosis rat model

BackgroundThe present study examined the effect of intermittent fasting (IF) on bone mineral content (BMC) and bone mineral density (BMD) and the markers of bone remodeling in a glucocorticoid-induced osteoporosis (GIO) rat model.MethodsForty male rats were allocated to 4 groups (N= 10 per group): control group of normal rats; control+ IF group (normal rats subjected to IF for 16–18 hr daily for 90 days); dexamethasone (DEX) group:(DEX [0.5 mg ip] for 90 days); and DEX+ IF group (DEX and IF for 90 days). By the end of the experiment, BMD and BMC in the right tibia were measured. Serum levels of the following were measured: glucose; insulin; triglycerides (TGs); total cholesterol; parathyroid hormone (PTH); osteoprotegerin (OPG); receptor activator of nuclear factor-κB (RANK); bone-resorbing cytokines, including bone deoxypyridinoline (DPD), N-terminal telopeptide of collagen type I (NTX-1), and tartrate …

Authors

Majed G Alrowaili,Abdelaziz M Hussein,Elsayed A Eid,Mohamed S Serria,Hussein Abdellatif,Hussein F Sakr

Journal

Journal of Bone Metabolism

Published Date

2021/11

Latest Reports on the Use of Lactic Acid Bacteria to Enhance Nutrient Consumption and Gut Health in the Natural Pigs

The Lactic Acid Bacteria strains (LAB), mainly Lactobacillus strains, endorse intestinal and serum immune responses to gastrointestinal diseases such as diarrhoea reduction effects and antitoxin rotavirus. Supplementation of diet with LAB can benefit growth performance; nutrient utilisation and nutrient digestibility therefore enhance gut health of pigs. Understanding in the narrative related to the advantageous consequences of consuming a range of LAB for swine production has been widely reviewed, and the hazard and the safety related issues to to use of LAB have also been considered in this review.Taking into consideration that the primary cost in a swine business is feed effectiveness, feed cost, holds an exceptionally, if not the supreme, consequence in industrial pig production. Worldwide, in accompany with other animal industries the swine industry is affecting towards the long run a total ban and in control on the usage of antibiotic growth promoters. For that reason, the selection of a perfect substitute to the in-feed antibiotics to reimburse for the vanished profits because of the ban on the usage of antibiotic is immediately required to maintain the business for the sustainable and advantageous swine production.

Authors

S Hussein,A Hussein,J Hailong

Published Date

2021

353-P: Safety and Tolerability of Sodium-Glucose Cotransporter 2 Inhibitors during Ramadan Fasting

Background: Diabetic management during Ramadan fasting is a significant clinical challenge. Sodium glucose co-transporter-2 (SGLT-2) inhibitors are new class of antidiabetic medications known for low frequency of associated hypoglycemia. The present study aimed to evaluate efficacy, safety and tolerability of SGLT-2 inhibitors in diabetic patients practicing Ramadan fasting.Patients and Methods: The study included 94 patients. They comprised 51 patients who received metformin and sulfonylureas: Glimepiride (1mg-6mg/d) or Gliclazide MR (60-120mg/d) and 43 patients who received SGLT2 inhibitors: Empagliflozin (25 mg), Dapagliflozin (10 mg) or Canagliflozin (300 mg). The study outcome parameters were frequency of hypoglycemia episodes, volume depletion episodes, number of days with early breaking of fasting and missed fasting days.Results: It was found that patients in SGLTI group experiences …

Authors

Elsayed Eid,Abdelaziz Hussein,Hossam A Ghazi

Journal

Diabetes

Published Date

2021/6/1

Effect of Stevia rebaudiana and exercise on fatty liver in type 2 diabetic rats

Introduction Nonalcoholic fatty liver disease (NAFLD) is a prevalent chronic liver disease, which threatens the health of both adults and children. It is strongly associated with type 2 diabetes mellitus, obesity and insulin resistance. In the present study, we evaluate the effect of Stevia rebaudiana and exercise on fatty liver in T2DM. Methods Thirty two male Sprague Dawely rats were divided into normal control, diabetic, Stevia treated diabetic, and exercise treated diabetic rats. Biochemical parameters, oxidative stress markers, and histopathological examination for liver tissue were done. Results in diabetic group, blood glucose level, HOMA index, cholesterol level, triglycerides level, bilirubin level, liver enzymes (ALT& AST), and MDA were significantly increased. While insulin level, GSH, and CAT activity were significantly decreased when compared to normal control group. Biochemical parameters and Oxidative stress markers were improved in the treated groups, the improvement was more significant in exercise treated group than Stevia treated group. Histopathological examination confirmed our results. Conclusion Stevia rebaudiana and exercise could protect against liver injury induced by T2DM, as they improve glycemic state, liver enzymes, and oxidative stress markers. Key words: NAFLD, T2DM, Stevia, Exercise.

Authors

Reem Mohie ElDin Emam,Asaad Elmileegy,Fayza R El-Menabawy,Gad El Mawla A Gad

Journal

Bulletin of Egyptian Society for Physiological Sciences

Published Date

2021/10/1

Curcumin offsets PTZ-induced epilepsy: involving inhibition of apoptosis, wnt/β-catenin, and autophagy pathways

Curcumin is a natural antioxidant isolated from Curcuma longa that proved to protect against experimentally induced epilepsy in animals. In the current work, we aimed to explore the possible molecular mechanisms of curcumin neuroprotective effect on PTZ-induced epilepsy in rats. Thirty male Sprague Dawley rats were subdivided into three groups: (a) normal control group, (b) PTZ group: rats received PTZ (50 mg/kg i.p. every other day) for 14 days, (c) curcumin + PTZ group: received PTZ as in PTZ group in addition to curcumin (200 mg/kg per oral daily) for 14 days. After each PTZ injection, the seizure stage, time to the first seizure and the duration of seizures were recorded. Also, at the end of experiment, oxidative stress markers, the expression of splitted caspase-3, β-catenin, LC3 (marker of autophagy), and alpha synuclein proteins were measured in brain tissues. treatment with curcumin caused significant …

Authors

Mona Hamdy,Ashraf Antar,Mohamed El-Mesery,Mohammed El-Shafey,Amr N Ali,Khaled M Abbas,Osama Ali Abulseoud,Abdelaziz M Hussein

Journal

Egyptian Journal of Basic and Applied Sciences

Published Date

2020/1/1

Exercise and Stevia rebaudiana (R) extracts attenuate diabetic cardiomyopathy in type 2 diabetic rats: possible underlying mechanisms

Background and Aims: In the current work, we studied the effects of exercise and stevia rebaudiana (R) extracts on diabetic cardiomyopathy (DCM) in type 2 diabetic rats and their possible underlying mechanisms. Methods: Thirty-two male Sprague Dawley rats were randomly allocated into 4 equal groups; a) normal control group, b) DM group, type 2 diabetic rats received 2 ml oral saline daily for 4 weeks, c) DM+ Exercise, type 2 diabetic rats were treated with exercise for 4 weeks and d) DM+ stevia R extracts: type 2 diabetic rats received methanolic stevia R extracts. By the end of the experiment, serum blood glucose, HOMA-IR, insulin and cardiac enzymes (LDH, CK-MB), cardiac histopathology, oxidative stress markers (MDA, GSH and CAT), myocardial fibrosis by Masson trichrome, the expression of p53, caspase-3, α-SMA and tyrosine hydroxylase (TH) by immunostaining in myocardial tissues were measured …

Authors

Abdelaziz M Hussein,Elsayed A Eid,Ismaeel Bin-Jaliah,Medhat Taha,Lashin S Lashin

Journal

Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders)

Published Date

2020/9/1

442-P: Comparative Study of the Effects of GLP-1 Analog and SGLT2 Inhibitor against Diabetic Cardiomyopathy in Type 2 Diabetic Rats: Possible Underlying Mechanisms

Objectives: In the present study we investigated the possible cardioprotective e ects of GLP-1 and SGLTi against diabetic cardiomyopathy (DCM) in type 2 diabetic rats and the mechanisms underlying these e ects.Methods: 32-male Sprague Dawley rats were randomly subdivided into 4 equal groups; a) control group, b) DM group, type 2 diabetic rats received 2ml oral saline daily for 4 weeks, c) DM+ GLP-1, type 2 diabetic rats were treated with GLP-1 analogue (liraglutide) at a dose of 75 µg/kg via SC injection for 4 weeks and d) DM+ SGLTi: type 2 diabetic rats received SGLT2 inhibitor (dapagli ozin) at a dose of 1mg/kg via oral gastric gavage. By the end of treatment (4 weeks), serum blood glucose, HOMA-IR, insulin and cardiac enzymes (LDH, CK-MB) were measured. Also, the cardiac histopathology, myocardial oxidative stress markers (MDA, GSH and CAT) and norepinephrine (NE), myocardial brosis, the expression of caspase-3, TGF-β, TNF-α and tyrosine hydroxylase (TH) in myocardial tissues were measured.Results: T2DM caused signi cant increase in serum glucose, HOMA-IR index, serum CK-MB and LDH (p< 0.05). This was associated with signi cant myocardial damage and brosis, elevation of myocardial MDA, NE with upregulation of myocardial caspase-3, TNF-α, TGF-β and TH and signi cant decrease in serum

Authors

Elsayed A Eid,Abdelaziz Hussein,Lashin Lashin,Medhat Taha

Journal

Diabetes

Published Date

2020/6/1

2137-PUB: Exercise and Stevia R. Extracts Attenuate Diabetic Cardiomyopathy in Type 2 Diabetic Rats via Upregulation of Nrf2 and Suppression of Oxidative Stress, Fibrosis …

Objectives: In the current work, we studied the effects of exercise and stevia extracts on diabetic cardiomyopathy in type 2 diabetic rats and their possible underlying mechanisms.Methods: Thirty-two male Sprague Dawley rats were randomly allocated into 4 equal groups; a) normal control group, b) DM group, type 2 diabetic rats received 2ml oral saline daily for 4 weeks, c) DM+ Exercise, type 2 diabetic rats were treated with exercise for 4 weeks and d) DM+ stevia extracts: type 2 diabetic rats received methanolic stevia extracts. By the end of experiment, serum blood glucose, HOMA-IR, insulin and cardiac enzymes (LDH, CK-MB), cardiac histopathology, oxidative stress markers (MDA, GSH and CAT), myocardial fibrosis by Masson trichrome, the expression of p53, α-SMA and tyrosine hydroxylase (TH) by immunostaining in myocardial tissues were measured. Also, the expression of nuclear factor erythroid-like …

Authors

Abdelaziz Hussein,Elsayed A Eid,Medhat Taha,Lashin Lashin

Journal

Diabetes

Published Date

2020/6/1

Impact of dehydroepiandrosterone (DHEA) on bone mineral density and bone mineral content in a rat model of male hypogonadism

Objectives The present study examined the effect DHEA (dehydroepiandrosterone) on bone mineral content (BMC) and bone mineral density (BMD) and biomarkers of bone remodeling in orchidectomized male rats. Material and Methods A total of 32 male rats were divided equally into four groups (n = 8): (i) control group (C), (ii) control treated with DHEA (Control + DHEA), (iii) orchidectomized (ORCH) group that underwent bilateral orchidectomy and (iv) orchidectomized (ORCH) rats treated with DHEA (ORCH+DHEA). DHEA treatment started 4 weeks after orchidectomy and continued for 12 weeks. After 12 weeks the bone mineral density (BMD) and bone mineral content (BMC) were assayed in the tibia and femur of the right hind limb of each rat. We also measured the serum levels of the bone turnover markers deoxypyridinoline (Dpd), N-telopeptide of type I collagen (NTx), alkaline phosphatase (ALP), tartrate-resistant acid phosphatase 5b (TRAP-5b) and osteocalcin (OC) as well as receptor activator of nuclear factor kappa B (RANK) and osteoprotegerin (OPG). Results Orchidectomy in rats caused significant reduction in BMD, BMC, serum levels of testosterone, PTH (parathyroid hormone), OPG, OC and ALP with significant rise in serum levels of TRAP-5B, RANK, Dpd and NTx1 (p < 0.05). On the other hand, DHEA therapy for 12 weeks caused significant improvement in all studied parameters except NTx1 (p < 0.05). Conclusions DHEA corrected hypogonadism-induced osteoporosis in male rats probably via inhibiting osteoclastogenesis, stimulating the activity of osteoblasts and stimulating the secretion of PTH and testosterone.

Authors

Hussein F Sakr,Abdelaziz M Hussein,Elsayed A Eid,Ammar Boudaka,Lashin S Lashin

Journal

Veterinary Sciences

Published Date

2020/11/23

Blockade of renin angiotensin system ameliorates the cardiac arrhythmias and sympathetic neural remodeling in hearts of type 2 DM rat model

Objective The present study was designed to investigate the effects of renin angiotensin system (RAS) blockade on cardiac arrhythmias and sympathetic nerve remodelling in heart tissues of type 2 diabetic rats. Methods Thirty-two male Sprague Dawley rats were randomly allocated into 4 equal groups; a) normal control group: normal rats, b) DM group; after type 2 diabetes induction, rats received 2ml oral saline daily for 4 weeks, c) DM+ ACEi: after type 2 diabetes induction, rats were treated with enalapril (10 mg/kg, orally for 4 weeks) and d) DM+ ARBs: after type 2 diabetes induction, rats were treated with losartan (30 mg/kg, orally for 4 weeks). Results In type 2 diabetic rats, the results demonstrated significant prolongation in Q-T interval and elevation of blood sugar, HOMA-IR index, TC, TGs, LDL, serum CK-MB, myocardial damage, myocardial MDA, myocardial norepinephrine and tyrosine hydroxylase (TH …

Authors

Yomna M Yehya,Abdelaziz M Hussein,Khaled Ezam,Elsayed A Eid,Eman M Ibrahim,Mohamed AFE Sarhan,Aya Elsayed,Mohamed E Sarhan

Journal

Endocrine, Metabolic & Immune Disorders-Drug Targets (Formerly Current Drug Targets-Immune, Endocrine & Metabolic Disorders)

Published Date

2020/4/1

Chronic valproic acid administration increases plasma, liver, and brain ammonia concentration and suppresses glutamine synthetase activity

Asymptomatic valproic acid (VPA)-induced hyperammonemia in the absence of liver impairment is fairly common. However, the underlying mechanisms through which VPA causes elevation in plasma ammonia (NH4) remains under investigation. Male Sprague Dawley rats (n = 72) were randomly allocated to receive VPA 400 mg/kg, 200 mg/kg, or vehicle IP daily for either 8, 14, or 28 consecutive days. The behavioral effects of VPA were assessed. Plasma, liver, and prefrontal cortex (PFC), striatum (Str), and cerebellum (Cere) were collected 1 h post last injection and assayed for NH4 concentration and glutamine synthetase (GS) enzyme activity. Chronic VPA treatment caused attenuation of measured behavioral reflexes (p < 0.0001) and increase in plasma NH4 concentration (p < 0.0001). The liver and brain also showed significant increase in tissue NH4 concentrations (p < 0.0001 each) associated with significant reduction in GS activity (p < 0.0001 and p = 0.0003, respectively). Higher tissue NH4 concentrations correlated with reduced GS activity in the liver (r = −0.447, p = 0.0007) but not in the brain (r = −0.058, p = 0.4). Within the brain, even though NH4 concentrations increased in the PFC (p = 0.001), Str (p < 0.0001), and Cere (p = 0.01), GS activity was reduced only in the PFC (p < 0.001) and not in Str (p = 0.2) or Cere (p = 0.1). These results suggest that VPA-induced elevation in plasma NH4 concentration could be related, at least in part, to the suppression of GS activity in liver and brain tissues. However, even though GS is the primary mechanism in brain NH4 clearance, the suppression of brain GS does not seem to be the main factor …

Authors

Abdelnaser A Badawy,Rasha Elghaba,Mohamed Soliman,Abdelaziz M Hussein,Sana A AlSadrah,Amira Awadalla,Osama A Abulseoud

Journal

Brain Sciences

Published Date

2020/10/21

Comparative study of the effects of GLP1 analog and SGLT2 inhibitor against diabetic cardiomyopathy in type 2 diabetic rats: possible underlying mechanisms

The present study investigated the possible cardioprotective effects of GLP1 and SGLT2i against diabetic cardiomyopathy (DCM) in type 2 diabetic rats and the possible underlying mechanisms. Methods Thirty-two male Sprague Dawley rats were randomly subdivided into 4 equal groups: (a) control group, (b) DM group, type 2 diabetic rats with saline daily for 4 weeks, (c) DM + GLP1, as DM group with GLP1 analogue (liraglutide) at a dose of 75 µg/kg for 4 weeks, and (d) DM + SGLT2i as DM group with SGLT2 inhibitor (dapagliflozin) at a dose of 1 mg/kg for 4 weeks. By the end of treatment (4 weeks), serum blood glucose, homeostasis model assessment insulin resistance (HOMA-IR), insulin, and cardiac enzymes (LDH, CK-MB) were measured. Also, the cardiac histopathology, myocardial oxidative stress markers (malondialdehyde (MDA), glutathione (GSH) and CAT) and norepinephrine (NE), myocardial fibrosis, the expression of caspase-3, TGF-β, TNF-α, and tyrosine hydroxylase (TH) in myocardial tissues were measured. Results T2DM caused significant increase in serum glucose, HOMA-IR, serum CK-MB, and LDH (p < 0.05). Also, DM caused significant myocardial damage and fibrosis; elevation of myocardial MDA; NE with upregulation of myocardial caspase-3, TNF-α, TGF-β, and TH; and significant decrease in serum insulin and myocardial GSH and CAT (p < 0.05). Administration of either GLP1 analog or SGLT2i caused a significant improvement in all studied parameters (p < 0.05). Conclusion We concluded that both GLP1 and SGLT2i exhibited cardioprotective effects against DCM in T2DM, with the upper hand for SGLT2i. This might …

Authors

Abdelaziz M Hussein,Elsayed A Eid,Medhat Taha,Rami M Elshazli,Raouf Fekry Bedir,Lashin Saad Lashin

Journal

Biomedicines

Published Date

2020/2/25

Possible mechanisms for the renoprotective effects of date palm fruits and seeds extracts against renal ischemia/reperfusion injury in rats

PurposeThis work investigates the possible renoprotective effects of date palm fruits and seeds extract against renal ischemia and their underlying mechanisms.Methods108-Sprague Dawle male rats were randomly allocated into 6 equal groups differently receiving aqueous or methanolic fruit and seed extracts. Assay of serum creatinine, BUN and TNF-α, morphological examination of the left kidney, markers of the redox state (MDA, CAT, and GSH), the expression of TNFα and Nrf2 genes at the level of mRNA, the expression of caspase-3 and TGF-β proteins by immunohistochemistry were performed.Results45-min renal I/R caused significant deterioration of kidney functions (increase in serum creatinine and BUN) and morphology (P < 0.001) and significant reduction in CAT activity and GSH levels with significant increase in serum TNF-α and MDA concentration and the expression of Nrf2, caspase-3, TNF-α, and …

Authors

Mansour A Alghamdi,Abdelaziz M Hussein,Laith N Al-Eitan,Eman Elnashar,Ahmed Elgendy,Asim M Abdalla,Seham Ahmed,Wael A Khalil

Journal

Biomedicine & Pharmacotherapy

Published Date

2020/10/1

Abdelaziz M. Hussein FAQs

What is Abdelaziz M. Hussein's h-index at Mansoura University?

The h-index of Abdelaziz M. Hussein has been 19 since 2020 and 22 in total.

What are Abdelaziz M. Hussein's top articles?

The articles with the titles of

Effect of intermittent fasting on adriamycin-induced nephropathy: Possible underlying mechanisms

Human Wharton's jelly-derived mesenchymal stromal stem cells preconditioned with valproic acid promote cell migration and reduce renal inflammation in ischemia/reperfusion …

LC/MS analysis of mushrooms provided new insights into dietary management of diabetes mellitus in rats

New Insight into the Possible Roles of L-Carnitine in a Rat Model of Multiple Sclerosis

Possible Role of Oxidative Stress and Nrf2/HO-1 Pathway in Pentylenetetrazole-induced Epilepsy in Aged Rats

Vanillic Acid Ameliorates Demyelination in a Cuprizone-Induced Multiple Sclerosis Rat Model: Possible Underlying Mechanisms

Effect of melatonin on alpha synuclein and autophagy in dopaminergic neuronal differentiation of adipose mesenchymal stem cells

Impact of Cerium Oxide Nanoparticles on Metabolic, Apoptotic, Autophagic and Antioxidant Changes in Doxorubicin-Induced Cardiomyopathy: Possible Underlying Mechanisms

...

are the top articles of Abdelaziz M. Hussein at Mansoura University.

What are Abdelaziz M. Hussein's research interests?

The research interests of Abdelaziz M. Hussein are: Renal Physiology, Neurophysiology, Endocrine Physiology

What is Abdelaziz M. Hussein's total number of citations?

Abdelaziz M. Hussein has 1,501 citations in total.

What are the co-authors of Abdelaziz M. Hussein?

The co-authors of Abdelaziz M. Hussein are Ahmed M. Harraz, MBBCh, MSc, MD, MRCS, FRCS, EBU, Azza Abdel-Aziz, Mohamed Sarhan.

    Co-Authors

    H-index: 21
    Ahmed M. Harraz, MBBCh, MSc, MD, MRCS, FRCS, EBU

    Ahmed M. Harraz, MBBCh, MSc, MD, MRCS, FRCS, EBU

    Mansoura University

    H-index: 18
    Azza Abdel-Aziz

    Azza Abdel-Aziz

    Mansoura University

    H-index: 9
    Mohamed Sarhan

    Mohamed Sarhan

    Mansoura University

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