Abdelaziz Abbad

Abdelaziz Abbad

Université Cadi Ayyad

H-index: 28

Africa-Morocco

Abdelaziz Abbad Information

University

Université Cadi Ayyad

Position

Faculty of Sciences Semlalia Morocco

Citations(all)

2551

Citations(since 2020)

1486

Cited By

1670

hIndex(all)

28

hIndex(since 2020)

23

i10Index(all)

56

i10Index(since 2020)

44

Email

University Profile Page

Université Cadi Ayyad

Abdelaziz Abbad Skills & Research Interests

Valorization and Conservation of Medicinal and Aromatic Plants

Top articles of Abdelaziz Abbad

Antihyperlipidemic effect and GC-MS analysis of phytoconstituents from Laurus nobilis essential oil in rat

This study aimed to evaluate the effect of Laurus nobilis (L. nobilis) essential oil (EOs) (80 mg/kg) on Triton WR-1339-induced dyslipidemia in Wistar rats. The effect of L. nobilis essential oil (80 mg/kg) on lipid and lipoprotein profile was examined on Triton WR-1339-induced dyslipidemia in rats. Furthermore, the phytochemical evaluation was performed by GC-MS. In Addition, the acute toxicity of this EO was evaluated at a dose of 2 g/kg. The results revealed that the main constituents of L. nobilis EO were 1,8-cineole (39.5%), linalool (13.09%), and a-terpineol (11.55%). Furthermore, the EO did not cause any signs of toxicity or mortality, and the acute lethal dose 50 (LD50) was estimated to be higher than 2 g/kg. L. nobilis EO ameliorated lipid parameters and atherogenic indices. In conclusion, the study demonstrates that L. nobilis essential oils possess antidyslipidemic activity in acute model of hyperlipidaemia.

Authors

Ayoub Amssayef,Bouchra Soulaimani,Adil Qabouche,Abdelaziz Abbad,Mohamed Eddouks

Journal

Natural Product Research

Published Date

2024/4/6

Origanum Compactum Essential Oil Ameliorates Triton WR-1339-Induced Hyperlipidemia in Rats: Chemical Composition and Acute Toxicity

The impact of Origanum compactum essential oil (EO) at 80 mg kg−1 on Triton WR-1339-induced hyperlipidemia in rats was investigated.The EO’s phytochemical composition revealed the predominance of carvacrol (35.71%), p-cymene (13.74%), and carvacrol methyl ether (11.71%). Triton WR-1339 led to elevations in total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), as well asatherogenic indices encompassing the atherogenic coefficient (AC), Castelli’s risk index I (CRI-I), and Castelli’s risk index II (CRI-II). Conversely, pretreatment with O. Compactum EO for sevendays exerted a preventive effect against the increase of TC, TGs, LDL-C, VLDL-C, AC, CRI-I.

Authors

Ayoub Amssayef,Bouchra Soulaimani,Adil Qabouche,Abdelaziz Abbad,Mohamed Eddouks

Journal

Journal of Herbs, Spices & Medicinal Plants

Published Date

2024/4/2

Optimization of herbicidal activity of essential oil mixtures from Satureja alpina, Thymus satureioides and Myrtus communis on seed germination and post-emergence growth of …

Essential oil-based herbicides are promising candidates for the development of safe and eco-friendly botanical pesticides. This study aimed to develop an efficacious preparation by combining the essential oils (EOs) of Satureja alpina (L.), Thymus satureioides Coss and Myrtus communis (L.) and assessing its effects on the germination and early seedling growth of the weed species Amaranthus retroflexus (L.). The result showed that all EOs tested individually and in different combinations, significantly inhibited the germination of A. retroflexus in dose-dependent manner, with median effective dose (ED50) values ranging from 1.4 to 2.18 μL/mL. The mixture design analysis indicated synergistic interactions between the studied EOs, with the optimal combination predicted to be 22% M. communis, 23% S. alpina and 55% T. satureioides. Remarkably, the predicted and validated data showed a significant lower ED50 …

Authors

Soukaina Miloudi,Imane Abbad,Bouchra Soulaimani,Abderrahim Ferradous,Abdelaziz Abbad

Published Date

2024/3/4

Mixture-efficient identification of high-order herbal essential oil synergy using a normalized DiaMOND design

In this study, the effect of high-order combinations of Rosmarinus officinalis L. (R) EO with other essential oils obtained from selected Moroccan medicinal and aromatic plants, namely, Thymus satureioides Coss. (T), Origanum compactum Benth. (O), Pelargonium roseum L. (P), Artemisia herba-alba Asso. (A), and Salvia officinalis L. (S), was evaluated. The chemical composition of EOs was determined by GC/MS. Their antibacterial effect was determined using the microdilution method, whereas their antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) methods. The type of interaction between EOs was evaluated by the diagonal measurement of n-way drug interactions (DiaMOND). Antioxidant and antibacterial activity results demonstrate that O. compactum, T. satureioides, and P. roseum EOs tested alone showed …

Authors

Meriem Rafya,Abdelaziz Abbad,Abdellatif Hafidi,Naïma Zehhar,Fatiha Benkhalti

Journal

Biochemical Systematics and Ecology

Published Date

2024/2/1

Chemical composition, insecticidal and allelopathic properties of essential oils obtained from wild and cultivated Moroccan Satureja calamintha (L.)

This study aimed to investigate the chemical composition and biopesticide potential of essential oils (EOs) obtained from wild and cultivated Moroccan Satureja calamintha. GC/MS analysis revealed the presence of 15 compounds, accounting for 99.88% and 98.14% of the total oils obtained from wild and cultivated plants, respectively. Pulegone (72.93–68.58%), menthone (12.07–10.15%), and menthol (6.31–9.83%) were found as the main constituents of S. calamintha EOs. The results showed that cultivation of the species induced an accumulation of menthol and inversely a decrease of pulegone content. The insecticidal assays showed that both oils obtained from wild and cultivated plant exhibited strong toxicity towards the three insects tested (Tribolium confusum (Duv.) Rhyzopertha dominica (F.) and Sitophilus oryzae (L.)), and that for the two tested methods (LD50 ranged from 0.004 µL/cm2 to 0.011 µL/cm2 …

Authors

Imane Abbad,Bouchra Soulaimani,Abdelaziz Abbad

Journal

Journal of Natural Pesticide Research

Published Date

2023/3/1

Comparative Evaluation of the Essential Oil Chemical Variability and Antimicrobial Activity between Moroccan Lavender Species

Background: The present study was conceived to compare the chemical composition and the related antimicrobial activity of essential oils (EOs) obtained from nine commonly used Moroccan lavender species, namely L. mairei, L. multifida, L. tenuisecta, L. maroccana, L. latifolia, L. pedunculata subsp. atlantica, L. stoechas, L. angustifolia and L. dentata. It aimed to classify these widely used Moroccan lavenders, based on their chemical profiles and antimicrobial effectiveness against some human pathogenic microorganisms.Methods: Plant aerial parts were steam-distilled and the obtained EOs were initially analyzed using gas chromatography/mass spectrometry (GC/MS), then investigated for their antimicrobial activity against six bacteria and four candida strains, using disc diffusion and broth microdilution methods.Results: The chemical analyses showed that all the EOs investigated were classified as oxygenated …

Authors

Bouchra Soulaimani,Imane Abbad,Ayoub Amssayef,Elena M Varoni,Marcello Iriti,Nour-Eddine Mezrioui,Lahcen Hassani,Abdelaziz Abbad

Journal

JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS

Published Date

2023/10/1

Molecular Survey and Microscopic Examination of Haemoparasites Infecting Lizards from Morocco

PurposeUnderstanding the factors influencing parasite diversification, their distribution and infection patterns among hosts provides valuable information on the impact of parasitism on natural populations and the dynamics and evolution of disease outbreaks. In this study, we investigated prevalence and intensity of infection by blood parasites, the diversity, distribution, host specificity and infection patterns of apicomplexan parasites in lizards of Morocco.MethodsSamples from lizard specimens were collected between 2019 and 2021 from different prospected localities in Morocco. In total, 1500 lizards were collected, and their blood and tail tips were screened for blood parasites.ResultsUsing a PCR approach, we detected two distinct apicomplexan parasite groups (haemogregarines and haemococcidians) and also Stramenopiles (Proteromonas). A significant difference was identified between hosts families, with no …

Authors

Omar Er-Rguibi,David James Harris,Abdessamad Aglagane,El-Mustapha Laghzaoui,Latifa Kimdil,Abdelaziz Abbad,El Hassan El Mouden

Journal

Acta Parasitologica

Published Date

2023/9

Effect of Adding Thymus leptobotrys on Extra Virgin Olive Oil’s Quality and Physicochemical Parameters

The present study aimed to evaluate the changes in extra virgin olive oil (EVOO) after enrichment treatments with Thymus leptobotrys, a Moroccan endemic aromatic plant. The EVOO sample was supplemented with two materials obtained from the aerial parts of T. leptobotrys: powder (2.5 and 5%) or essential oil (100 and 200 ppm). The chemical composition analysis of T. leptobotrys extracts (essential oil and powder methanolic extract) allowed the determination of its phenolic profile. Quality parameters (acidity, peroxide value, and specific extinction coefficients), color, fatty acids composition, minor components (pigments and phenol), and Rancimat oxidative stability of the control and enriched oils were measured. Rosmarinic acid and thymusin were the main identified compounds in the powder; thymusin was identified for the first time in the essential oil where it was predominant. Generally, the enrichments did not downgrade the oil from its initial category (EVOO), according to quality indexes. Adding T. leptobotrys to EVOO, especially the essential oil, enriched its phenolic profile by allowing the migration of thymusin, a lipophile flavone, without affecting its quality parameters or color.

Authors

Aouatif Aboudia,Manal Zefzoufi,Meriem Rafya,Naïma Zehhar,Abderraouf El Antari,Abdelaziz Abbad,Fatiha Benkhalti

Journal

Journal of Food Quality

Published Date

2023/6/2

Arbuscular mycorrhizal fungi associated with endemic Moroccan lavender (Lavandula maroccana Murb.): effects on plant growth, volatile oil composition and …

The increasing demand for Moroccan Lavender (Lavandula maroccana) accompanied by the drastic decline of its wild populations, requires a sustainable exploitation of this vulnerable Moroccan endemic medicinal plant. The aim of this study was to assess the effect of cultivation and the application of arbuscular mycorrhizal fungi (AMF) on the plant growth, chemical profile and the antimicrobial properties of volatile oil of L. maroccana. The results revealed that the inoculated plants achieved the highest biomass production. GC/MS investigation identified 48 compounds, representing 97.73% - 97.85% of the total essential oils (EOs), with carvacrol (44.58% - 55.12%), p-cymen-8-ol (4.84% - 6.41%), α-farnesene (4.11% - 4.74%) and 2-methoxy-4-vinylphenol (3.71% - 5.35%) as major constituents. From the comparative chemical compositions of cultivated and wild lavender EOs, it appears that cultivation induced a …

Authors

Bouchra Soulaimani,Abdelilah Meddich,Soufiane Lahbouki,Imane Abbad,Abderrahim Ouarghidi,Nour Eddine Mezrioui,Lahcen Hassani,Abdelaziz Abbad

Journal

Journal of Essential Oil Bearing Plants

Published Date

2023/5/4

New locality and host records of mites and ticks (Chelicerata: Acari) parasitizing lizards of Morocco

The aim of this study was to investigate the parasitic relationships of mites and ticks infesting lizards in Morocco and their determinant factors. A total of 1498 lizards representing 27 species, 8 families and 18 genera were randomly captured and examined in their natural habitats. The overall prevalence of ectoparasite infestation was 22.4%, with a mean intensity of 14.5 (95% CI: 12.2 – 16.8). Four families of Acari (Chelicerata) were identified: Ixodidae, Macronyssidae, Pterygosomatidae, and Trombiculidae, with eleven new host records and new data on geographical distributions. Trombiculid mites were the most prevalent (17.2%), followed by the Pterygosomatidae (4.1%), the Ixodidae (1.5%), and the Macronyssidae (0.4%). Nevertheless, Macronyssids showed the highest mean intensity 31.7 (14.3 – 54.2), followed by the Pterygosomatidae 22.0 (16.2 – 30.5), the Trombiculidae 12.7 (10.9 – 14.8), and the Ixodidae 1.7 (1.3 – 2.2). In total, nine species of parasites were found, and co-infestations were recorded on three lizard species. Ixodid prevalence and intensity were significantly related to altitude and host species, whereas Macronyssid prevalence and intensity were associated with host age and altitude. Ectoparasite intensity was associated with lizard sex for all mite families, with males carrying heavier loads than females, while prevalence was related to sex only for Pterygosomatid and Trombiculid mites. Season was important for Macronyssid and Trombiculid infestation intensity. Finally, host characteristics (species, size, age class), along with altitude, were determinant factors for infestation prevalence and intensities in Pterygosomatid and …

Authors

Omar Er-Rguibi,El-Mustapha Laghzaoui,Abdessamad Aglagane,Latifa Kimdil,Alexandr A Stekolniko,Abdelaziz Abbad

Journal

Acarologia

Published Date

2023/4/12

Antibacterial Activity of Essential Oils Combinations based on Thymus broussonnetii, and Their Synergism with some Antibiotics

The present study aimed to evaluate the antibacterial activity of the essential oil (EO) of Moroccan endemic Thymus broussonnetii alone, and in combination with EOs obtained from selected medicinal plants, namely Myrtus communis, Artemisia herba alba, Thymus pallidus, Thymus satureioides, Teucrium polium, and Rosmarinus officinalis. The synergistic interactions between the most effective combinations based on T. broussonnetii EO with two conventional antibiotics (streptomycin and ciprofloxacin) were also investigated. T. broussonnetii EO possessed a higher inhibitory activity against tested pathogenic bacteria with inhibition zone diameter (IZ) ranging from 21.61 ± 0.03 to 40.09 ± 0.02 mm, and MIC values between 0.140 mg/mL and 0.280 mg/mL. M. communis, A. herba alba, T. pallidus, T. satureioides, T. polium, and R. officinalis EOs showed moderate to weak antibacterial activity. Among tested EO …

Authors

Oumaima Amassmoud,Imane Abbad,Marcello Iriti,Lahcen Hassani,Noureddine Mezrioui,Abdelaziz Abbad

Journal

Current Microbiology

Published Date

2023/12

Optimization of the insecticidal response of combined essential oils from Satureja alpina, Rosmarinus officinalis and Ammodaucus leucotrichus using a designed …

The main objective of the present study is to investigate the fumigant toxicity of EOs extracted from Satureja alpina (L.) Scheele (Lamiaceae), Rosmarinus officinalis (L.) (Lamiaceae) and Ammodaucus leucotrichus(Coss. & Durieu) (Apiaceae) and to predict specific EO mixtures that provide optimal insecticidal activities against two common secondary insect pests, namely Stegobium paniceum (L.) (Coleoptera: Anobiidae) and Tribolium confusum du Val (Coleoptera: Tenebrionidae). GC/MS analysis showed that A. leucotrichus EO was characterized by the abundance of L-perillaldehyde and D-limonene, while the main constituents of R. officinalis EO were 1.8-cineole, camphor, and α-pinene. S. alpina EO was mainly composed of pulegone. Concerning the insecticidal activity, the results showed that all EOs tested individually exerted high efficacy, with LC50 from 3.4 to 6.6 µL/L air for S. paniceum, and from 25.3 to 41 …

Authors

Bouchra Soulaimani,El-Mustapha Laghzaoui,Mohamed Ait Babram,Robert Spooner-Hart,Lahcen Hassani,Abdelaziz Abbad

Journal

International Journal of Tropical Insect Science

Published Date

2022/6

Cross-sectional study of ticks in the vulnerable free-living spur-thighed tortoise Testudo graeca (Testudines: Testudinidae) from Morocco

The spur-thighed tortoise (Testudo graeca L., 1758) is classified as a vulnerable species by the IUCN. In this context, it is important to explore the factors affecting its populations to set up an effective conservation plan. The present study was carried out in seven localities of Morocco with the objective to determine the ticks parasitizing this tortoise and their prevalence in relation to host characteristics and environmental factors. We examined 392 free-living tortoises for the presence of ticks during the spring 2018. A total number of 2238 adult ticks (no immature tick detected) were collected and all identified as Hyalomma aegyptium. Infection with at least one tick was detected in 77.6% ± 6.5 of the total tortoises. The infestation prevalence varied according to localities. Infestation intensity varied from 1.5 to 12, while tick abundance ranged from 0.65 to 12 ticks/tortoise. Male tortoises were more infested than females (P < …

Authors

El-Mustapha Laghzaoui,Abdellah Bouazza,Abdelaziz Abbad,El Hassan El Mouden

Journal

International Journal of Acarology

Published Date

2022/1/2

Optimization of antibacterial activity of essential oil mixture obtained from three medicinal plants: Evaluation of synergism with conventional antibiotics and nanoemulsion …

The present study aimed to optimize the antibacterial activity of essential oil (EO) mixture obtained from Ammodaucus leucotrichus Cosson, Thymus vulgaris L. and Lavandula maroccana (Murb.) against three multi-drug resistant bacteria, using augmented simplex-centroid design. GC–MS analysis showed that A. leucotrichus EO was characterized by the abundance of l-perillaldehyde (50.39%), d-limonene (21.61%) and bornyl angelate (7.09%). T. vulgaris EO was dominated by thymol (42.34%), p-cymene (19.71%) and γ-terpinene (11.50%), while carvacrol (71.62%) represents the main component of L. maroccana EO. Regarding antibacterial assays, individual EOs displayed moderate activity with MIC and MBC values ranging from 0.06% to 5.00%, while the coefficient terms of the different tested combinations revealed synergistic interactions. The results of predicted optimization showed that the optimal EOs …

Authors

Bouchra Soulaimani,Imane Abbad,Elena Varoni,Marcello Iriti,Nour-Eddine Mezrioui,Lahcen Hassani,Abdelaziz Abbad

Journal

South African Journal of Botany

Published Date

2022/12/1

A phylogenetic assessment of nematodes (Oxyuroidea: Pharyngodonidae) infecting Moroccan lizards

Molecular tools can be used to estimate the phylogeny of species and to identify cryptic diversity, but their use for parasites has lagged behind that of free-ranging organisms. As an example, in North Africa there is minimal molecular data available for helminth parasites of lizards. In this work we used two molecular markers (the nuclear 18S rRNA and the mitochondrial Cytochrome c Oxidase subunit 1) to investigate the diversity of nematodes of the family Pharyngodonidae parasitizing three genera of lizards from Morocco (Chalcides, Quedenfeldtia and Tarentola) and to explore their co-evolutionary history. Morphological assessments indicated that members of three genera were present: Spauligodon, Thelandros, and Parapharyngodon. Phylogenetic analysis of 18S rRNA sequences indicated the monophyly of the genus Spauligodon, and that some lineages could be distinguished, including Spauligodon auziensis from the host species Tarentola mauritanica, and another unnamed lineage from hosts of the genus Chalcides. However, with this slow-evolving marker some species could not be distinguished. The genus Thelandros was not monophyletic, although relationships were not strongly supported. Analysis of the faster evolving mitochondrial marker clearly separated various species of Spauligodon, as well as distinct unnamed lineages identified in the host genus

Authors

O Er-Rguibi,DJ Harris,A Aglagane,EM Laghzaoui,L Kimdil,A Abbad,EH El Mouden

Journal

Helminthologia

Published Date

2022/9/1

Acaricidal activity of Mentha suaveolens subsp. timija, Chenopodium ambrosioides, and Laurus nobilis essential oils, and their synergistic combinations against the …

The main objective of the present study was to evaluate under laboratory conditions the toxicity of essential oils (EOs) extracted from Mentha suaveolens L. subsp. timija (Briq.) Harley, Chenopodium ambrosioides L. and Laurus nobilis L. against Varroa destructor adult mites. Also, various combinations were prepared by mixing equal concentrations of any two and all three studied EOs and used to evaluate the combinational acaricidal effects of mixtures against adult Varroa mites. The GC/MS analysis indicated that the main chemical compounds of M. suaveolens subsp. timija EO were menthone (40.42%) and pulegone (19.22%), whereas C. ambrosioides EO is rich in α-terpinene (34.08%), isoascaridol (13.6%), p-cymene (10.95%), thymol (10.26%), ascaridole (10.25%) and carvacrol (7.75%). L. nobilis EO is mainly composed with 1,8-cineol (37.5%) and linalool (14.09%). The fumigation bioassays showed that the …

Authors

Abdessamad Aglagane,El-Mustapha Laghzaoui,Bouchra Soulaimani,Omar Er-Rguibi,Abdelaziz Abbad,El Hassan El Mouden,Mohamed Aourir

Journal

Journal of Apicultural Research

Published Date

2022/1/1

Insecticidal activity of some plant essential oils against the Opuntia cochineal scale insect, Dactylopius opuntiae Cockerell (Hemiptera: Dactylopiidae)

Opuntia ficus-indica (L.) is largely cultivated in Morocco because of its remarkable adaptation to arid and semi-arid climates and significant economic importance. However, in recent years, a severe invasion of these species plantations by Dactylopius opuntiae has been detected in many regions in Morocco. The present study evaluates under laboratory conditions the toxicity of some Moroccan plant essential oils (EOs) against the mobile stages (adult males and crawlers) of D. opuntiae in direct contact and fumigation bioassays. All EOs tested were found to be toxic to adult males and crawlers of D. opuntiae, but with variable degrees. Thymus vulgaris EO exhibited the highest activity against adult males in both contact and fumigation assays. EOs obtained from Mentha suaveolens subsp. timija, Chenopodium ambrosioides, Mentha piperita, Myrtus communis and Laurus nobilis expressed a moderate activity …

Authors

El-Mustapha Laghzaoui,Abdessamad Aglagane,Bouchra Soulaimani,Imane Abbad,Latifa Kimdil,Omar Er-Rguibi,Abdelaziz Abbad,El Hassan El Mouden

Journal

Phytoparasitica

Published Date

2022/9

New host and locality records of helminths’ infection of seven lizards from Morocco

Morocco has a great diversity of reptiles; more than 26% of the herpetofauna species are considered to be endemic. Nevertheless, there is little information available on helminth parasites of Moroccan lizards. The purpose of this article is to establish a helminth list using non-invasive methods for natural populations of seven lizard species: Acanthodactylus maculatus, Chalcides mionecton, Chalcides montanus, Chalcides polylepis, Quedenfeldtia moerens, Quedenfeldtia trachyblepharus, and Tarentola mauritanica. For each species, prevalence and intensity of infection were given as well as their relationship to some determinant parameters (sex, age class, size, altitude, and seasons). Four species of nematodes, Parapharyngodon micipsae, Pharyngodon mamillatus, Spauligodon auziensis, Thelandros alatus, and unassigned Spauligodon were found. Mean helminth intensity for the seven lizard species was 7.3 …

Authors

Omar Er-Rguibi,Charles Robert Bursey,El-Mustapha Laghzaoui,Abdessamad Aglagane,Latifa Kimdil,Abdelaziz Abbad,El Hassan El Mouden

Journal

Parasitology Research

Published Date

2022/9

Synergistic Anticandidal Effects of Six Essential Oils in Combination with Fluconazole or Amphotericin B against Four Clinically Isolated Candida Strains

The development of opportunistic pathogenic Candida strains insensitive to several classes of antifungals has emerged as a major health care problem during the last years. Combinational therapy of natural products (e.g., essential oils, EOs) with conventional antifungals has been suggested as a promising alternative to overcome this medical problem. The present study investigates the potential antifungal activity of EOs extracted from some selected medicinal plants, alone and in combination with two common conventional antifungals (fluconazole and amphotericin B) against four clinical Candida isolates. MIC assays indicated that EOs induced strong anticandidal activities with MIC values ranging from 0.162 to 4.950 mg/mL. The combination of amphotericin B with Thymus leptobotrys, Origanum compactum and Artemisia herba alba EOs provided a synergistic effect against C. krusei only, with MIC gain of four-fold, and additive effect against remaining strains (MIC gain = two-fold). Interesting synergistic interactions were observed by combining all studied EOs with fluconazole, with reduction rates of their MICs ranging from 16 to 512-fold. This synergistic effect was very pronounced with the combination of T. leptobotrys EO and fluconazole. These findings indicate that studied EOs can be used as anti-candidals in combination with antifungals, particularly fluconazole, to counteract the emergence of resistant Candida spp.

Authors

Bouchra Soulaimani,Elena Varoni,Marcello Iriti,Nour-Eddine Mezrioui,Lahcen Hassani,Abdelaziz Abbad

Journal

Antibiotics

Published Date

2021/8/27

Essential oils as sustainable control agents against Varroa destructor (Acari, Varroidae), an ectoparasitic mite of the western honeybees Apis mellifera (Hymenoptera: Apidae …

The western honeybee (Apis mellifera L.) is one of the most important insect species that, unfortunately, is being confronted with multiple biological stressors that threaten their existence. Varroa destructor (Anderson and Trueman) is the most devastating ectoparasitic mite known today. In this review, we offer an up-to-date review of the literature on the use of essential oils (EOs) against V. destructor, particularly the last decade (2010-onwards). We find that 40 aromatic plants were extracted for EOs and screened for their varroacidal activity under laboratory and/or field conditions. Through this review, we urge the scientific community to pay more attention to the following points: 1) the laboratory bioassays should be completed with field trials as they are the ones capable of revealing the true potency of the studied EO, 2) sub-lethal effects of the screened EO on honeybees as non-target organisms as well as their …

Authors

Abdessamad Aglagane,El-Mustapha Laghzaoui,Sana Ben Elfakir,Omar Er-Rguibi,Abdelaziz Abbad,El Hassan El Mouden,Mohamed Aourir

Published Date

2021/7/4

Determinants of prevalence and co-infestation by ecto- and endoparasites in the Atlas day gecko, Quedenfeldtia trachyblepharus, an endemic species of Morocco

The present work was undertaken to investigate the occurrence of ectoparasites (mites and ticks) and endoparasites (haemogregarines and helminths) in the Atlas day gecko, Quedenfeldtia trachyblepharus (Boettger, 1874), a high-altitude Moroccan endemic lizard. The study examinated also the effect of some host parameters (age, sex, size, body condition), in addition to the season and altitude on the prevalence and intensity of parasite infestations. The study was conducted in three localities from May to September 2019. The results indicated that 35% of juvenile geckos were found to be parasited by one type of parasite. Contrarily, up to three types of parasites were detected in the adults. The prevalence of mite infestations was 48.02%, with a mean intensity of 11.80 ± 15.69. The patterns of mite infestations was found to be mainly related to the altitude, while the prevalence and intensity of infestations …

Authors

Omar Er-Rguibi,El-Mustapha Laghzaoui,Abdessamad Aglagane,Latifa Kimdil,Abdelaziz Abbad,El Hassan El Mouden

Journal

Parasitology Research

Published Date

2021/7

Combined antibacterial activity of essential oils extracted from Lavandula maroccana (Murb.), Thymus pallidus Batt. and Rosmarinus officinalis L. against antibiotic-resistant …

IntroductionCombinations of plant essential oils (EOs) provide a novel approach to increasing effectiveness against resistant bacteria. In this context, the combined antibacterial effects of EOs obtained from Lavandula maroccana (Murb.), Thymus pallidus Batt., and Rosmarinus officinalis L. against three multi-drug resistant Gram-negative bacteria were evaluated.MethodsThe antibacterial effect of each EO alone was evaluated by agar diffusion and broth microdilution methods, while the effects of EO binary combinations were evaluated through the determination of the fractional inhibitory concentration index (FICI) using the checkerboard method.ResultsGC-MS analysis of EOs showed that T. pallidus was dominated by γ-terpinene, thymol and p-cymene, while the main constituents of R. officinalis were 1,8-cineole, camphor, and α-pinene. L. maroccana EO was characterized by a high percentage of carvacrol. EOs …

Authors

Bouchra Soulaimani,Najoua El Hidar,Sana Ben El Fakir,Noureddine Mezrioui,Lahcen Hassani,Abdelaziz Abbad

Journal

European Journal of Integrative Medicine

Published Date

2021/4/1

Chemical composition, antibacterial activity and effect of Rhus albida Schousb essential oil on the inhibition of NorA efflux pump in Staphylococcus aureus

Human infectious diseases once treatable with antibiotics are now becoming a serious life-threatening problem due to the emergence of multidrug resistant (MDR) micro-organisms. For this, essential oils (EOs) could constitute a viable source of bioactive compounds capable of inhibiting the mechanisms of this antimicrobial resistance.In this context, our goals were to determine for the first time the chemical composition of EO extracted from aerial parts of Rhus albida, its antibacterial and synergistic interactions with fluoroquinolones and finally to evaluate its ability to inhibit the Staphylococcus aureus NorA efflux pump.The chemical profile and antibacterial activity of R. albida EO were determined using Gas Chromatography coupled to Mass Spectrometry and microdilution assays, respectively. While its NorA efflux-pump-inhibiting activity was assessed using a fluorescent real-time efflux method (based on ethidium …

Authors

Najoua Elhidar,Bouchra Soulaimani,André Goehler,Jürgen A Bohnert,Abdelaziz Abbad,Lahcen Hassani,Nour-Eddine Mezrioui

Journal

South African Journal of Botany

Published Date

2021/11/1

Novel DiOC3 96-well real-time efflux assay for discovery of NorA efflux pump inhibitors in Staphylococcus aureus

The NorA efflux pump is one of the most studied efflux systems in Staphylococcus aureus and confers multidrug resistance to a variety of dyes and antimicrobial compounds. Hence, inhibition of the NorA efflux pump might be a viable option for restoring susceptibility to antibiotics like fluoroquinolones. Fluorescent real-time efflux assays are important tools to identify putative efflux pump inhibitors. Nevertheless, the number of available compounds for usage in Staphylococcus aureus is limited. Previously, a 3-dipropyloxacarbocyanine iodide (DiOC3) efflux assay was published that circumvented problems associated with the usage of ethidium bromide, namely slow efflux and suggested mutagenicity. However, the DiOC3 assay protocol was cuvette – based and therefore needs to be adapted to the 96-well plate format. Hence, we optimized this assay for usage with 96-well plates. The new assay allows for rapid high …

Authors

Najoua Elhidar,Ahmed Nafis,André Goehler,Abdelaziz Abbad,Lahcen Hassani,Nour-Eddine Mezrioui,Jürgen A Bohnert

Journal

Journal of Microbiological Methods

Published Date

2021/2/1

New insight into antimicrobial activities of Linaria ventricosa essential oil and its synergetic effect with conventional antibiotics

The purpose of the present study was to determine for the first time the volatile constituents, the antioxidant and antimicrobial activities of the essential oil (EO) of the endemic Moroccan Linaria ventricosa, alone or in combination with four known antibiotics. The major constituents were 2-methoxy-4-vinylphenol (17.4%), α-terpinene (13.64%) and 3,5-dimethylphenyl isocyanate (12.21%). The EO had moderate antioxidant potency, as measured by DPPH free radical scavenging (1.233 ± 0.031 mg/mL), ferric reducing antioxidant power assay (0.373 ± 0.019 mg/mL) and β-carotene/linoleic acid (0.922 ± 0.026 mg/mL). EO showed microbicidal activity against all microorganisms tested. The highest effectiveness was recorded against Candida albicans (IZ = 24 mm, MIC = 4.87 mg/mL and MMC = 9.75 mg/mL) and Candida glabrata (IZ = 22 mm, MIC = MMC = 4.87 mg/mL). Gram negative …

Authors

Ahmed Nafis,Fatima Ezzahraa Saad,Fatima El Khalloufi,Ayoub Kasrati,Abdelaziz Abbad,Noureddine Mezrioui,Brahim Oudra,Vitor Vasconcelos,Lahcen Hassani

Journal

Archives of microbiology

Published Date

2021/9

Prevalence and intensity of gastrointestinal parasites in the vulnerable spur-thighed tortoise (Testudo graeca) from the central-western of Morocco

The aim of this study was to determine the prevalence and intensity of gastrointestinal parasites in donkeys slaughtered in Kinamba, Mogotio, and Lodwar slaughterhouses and their association with several host factors. A survey was done between July and September 2017 in three slaughterhouses. Faecal samples were collected per rectum from all the study donkeys for faecal egg counts (EPG) and morphological identification of the eggs. At slaughter, the gastrointestinal tracts were opened and examined visually, and all helminth parasites collected were subjected to morphological identification. Prevalence and intensity were calculated based on the helminth identified and EPG. A total of 282 donkeys were sampled. A majority of the donkeys (89%) were in poor body condition. Ten helminth parasite species were identified in 85.5% donkeys. They were Strongylus vulgaris (52.8%), Parascaris equorum (20.2%), Strongylus edentatus (12.1%), Anaplocephala perfoliata (10.3%), Setaria equina (3.5%) Anaplocephala magna (2.5%), Cylicocyclus auriculatus (2.1%), Cyathostomum species (1.8%), Strongylus equinus (0.4%), and Triodontophorus serratus (0.4%). A significant percentage (55.3%) had no eggs in their feces, 39% had low infection, 5% had medium, and only 0.7% were heavily infected. Prevalence rates via use of the EPG showed Strongyles (44.7%), Parascaris equorum (5.3%), Oxyuris equi (11%), Triodontophorus tenuicolis (0.7%), Habronema species (0.7%), and cestodes eggs (0.4%). No significant differences were observed between fecal Strongylus egg count and age, sex, and pregnancy status. However, donkeys with poor …

Authors

Nancy Mulwa,Samuel Githigia,Davis Karanja,Cecilia Mbae,Eberhard Zeyhle,Erastus Mulinge,Japhet Magambo,Kennedy Ogola

Journal

Scientifica

Published Date

2020/3/9

Absence of Hemolivia mauritanica (Apicomplexa: Haemogregarinidae) in natural populations of Testudo graeca in Morocco

During spring 2018, we captured 101 spur-thighed tortoises, Testudo graeca, from seven localities in central Morocco. All tortoises were examined for the presence of blood parasites Hemolivia mauritanica and Hyalomma aegyptium ticks, the known vectors. We looked for H. mauritanica infection by examination of blood smears and by genetic analysis with PCR using extractions from both tortoises and ticks. On all tortoises collected, 71.29% were infested with at least one tick, with a mean infestation intensity of 7.12 ticks/tortoise and maximum of 15.55 ticks/tortoises in Had Draa locality (Essaouira region). Although the definitive host is present and abundant in all tortoise populations, all blood samples were Hemolivia-negative. Our results support and confirm the finding of studies previously conducted in other populations of Morocco and indicate that H. mauritanica has a narrower distribution range than its tick …

Authors

El-Mustapha Laghzaoui,Dimitra Sergiadou,Ana Perera,D James Harris,Abdelaziz Abbad,El Hassan El Mouden

Journal

Parasitology Research

Published Date

2020/12

Host-parasite association of Placobdella costata (Glossiphoniidae: Hirudinea) and Mauremys leprosa (Geoemydidae: Testudinoidea) in aquatic ecosystems of …

Emys orbicularis (Linnaeus, 1758) was considered as a specific host of Placobdella costata (Fr. Mûller, 1846). However, since the parasite was recorded from outside the distribution area of its host, some authors suggested a possible relationship with other hosts. Although two accidental associations were found with another turtle, Mauremys leprosa (Schweigger, 1812), the obtained data remain insufficient to better understand this discovered host-parasite ecological system. In this context, the present study was carried out to evaluate the relationship between the Mediterranean pond turtle, M. leprosa, and the freshwater rhynchobdellid leech, P. costata (Hirudinida: Glossiphoniidae), in aquatic ecosystems of Morocco. During the period from April to June 2018, we found leeches attached to turtles in five out of 30 populations sampled with a prevalence of infection significantly higher in adult than that in …

Authors

El-Mustapha Laghzaoui,Abdelaziz Abbad,El Hassan El Mouden

Journal

Parasitology Research

Published Date

2020/10

Seaweed essential oils as a new source of bioactive compounds for cyanobacteria growth control: innovative ecological biocontrol approach

The application of natural compounds extracted from seaweeds is a promising eco-friendly alternative solution for harmful algae control in aquatic ecosystems. In the present study, the anti-cyanobacterial activity of three Moroccan marine macroalgae essential oils (EOs) was tested and evaluated on unicellular Microcystis aeruginosa cyanobacterium. Additionally, the possible anti-cyanobacterial response mechanisms were investigated by analyzing the antioxidant enzyme activities of M. aeruginosa cells. The results of EOs GC–MS analyses revealed a complex chemical composition, allowing the identification of 91 constituents. Palmitic acid, palmitoleic acid, and eicosapentaenoic acid were the most predominant compounds in Cystoseira tamariscifolia, Sargassum muticum, and Ulva lactuca EOs, respectively. The highest anti-cyanobacterial activity was recorded for Cystoseira tamariscifolia EO (ZI = 46.33 mm, MIC = 7.81 μg mL−1, and MBC = 15.62 μg mL−1). The growth, chlorophyll-a and protein content of the tested cyanobacteria were significantly reduced by C. tamariscifolia EO at both used concentrations (inhibition rate >67% during the 6 days test period in liquid media). Furthermore, oxidative stress caused by C. tamariscifolia EO on cyanobacterium cells showed an increase of the activities of superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA) concentration was significantly elevated after 2 days of exposure. Overall, these experimental findings can open a promising new natural pathway based on the use of seaweed essential oils to the fight against potent toxic harmful cyanobacterial blooms (HCBs).

Authors

Soukaina El Amrani Zerrifi,Fatima El Khalloufi,Richard Mugani,Redouane El Mahdi,Ayoub Kasrati,Bouchra Soulaimani,Lillian Barros,Isabel CFR Ferreira,Joana S Amaral,Tiane Cristine Finimundy,Abdelaziz Abbad,Brahim Oudra,Alexandre Campos,Vitor Vasconcelos

Journal

Toxins

Published Date

2020/8/17

A case of massive infestation of a female Spur-thighed tortoise Testudo graeca by blood-sucking ticks Hyalomma aegyptium (Acari: Ixodidae)

Infestation by ticks affects several vertebrate groups, including tortoises. Testudo species are among chelonian fauna that are infested in their natural habitat by several tick species, with Hyalomma aegyptium as the dominant one. We report a heavy tick infestation found in female T. graeca in May 2018 in Had Draa (Essaouira region, Morocco). The female, with carapace length of 175 mm and body weight of 779.3 g was found to be infested by 288 H. aegyptium in different stages. It was more infested by female ticks than males, and the hind limbs (59.6%) and forelimbs (30.25%) were the most frequent predilection sites of ticks. Our data provide the first record of massive parasitism by H. aegyptium in this vulnerable and threatened tortoise.

Authors

El Hassan El Mouden,El-Mustapha Laghzaoui,Abderrafea Elbahi,Abdelaziz Abbad

Journal

International journal of acarology

Published Date

2020/1/2

Essential oils from Moroccan plants as promising ecofriendly tools to control toxic cyanobacteria blooms

The use of natural compounds obtained from many plant resources are considered the most promising and eco-friendly alternative approach for controlling harmful algae in aquatic ecosystems. In this study, the anti-cyanobacterial activity of essential oils (EOs) extracted from seven Moroccan aromatic and medicinal plants against unicellular Microcystis aeruginosa has been evaluated. The chemical composition of EOs was analyzed by GC–MS, and a total of 55 constituents were identified accounting for 97.6% to 100% of the total oils. The potential algicidal activity was evaluated qualitatively by the application of the paper disk diffusion method in solid medium and quantitatively by the application of broth microdilution methods in liquid medium. The results of solid medium showed that Chenopodium ambrosioides, Thymus broussonetii, Thymus maroccanus and Thymus satureioides essential oils inhibited totally the …

Authors

Soukaina El Amrani Zerrifi,Ayoub Kasrati,Zakaria Tazart,Fatima El Khalloufi,Abdelaziz Abbad,Brahim Oudra,Alexandre Campos,Vitor Vasconcelos

Journal

Industrial Crops and Products

Published Date

2020/1/1

Abdelaziz Abbad FAQs

What is Abdelaziz Abbad's h-index at Université Cadi Ayyad?

The h-index of Abdelaziz Abbad has been 23 since 2020 and 28 in total.

What are Abdelaziz Abbad's top articles?

The articles with the titles of

Antihyperlipidemic effect and GC-MS analysis of phytoconstituents from Laurus nobilis essential oil in rat

Origanum Compactum Essential Oil Ameliorates Triton WR-1339-Induced Hyperlipidemia in Rats: Chemical Composition and Acute Toxicity

Optimization of herbicidal activity of essential oil mixtures from Satureja alpina, Thymus satureioides and Myrtus communis on seed germination and post-emergence growth of …

Mixture-efficient identification of high-order herbal essential oil synergy using a normalized DiaMOND design

Chemical composition, insecticidal and allelopathic properties of essential oils obtained from wild and cultivated Moroccan Satureja calamintha (L.)

Comparative Evaluation of the Essential Oil Chemical Variability and Antimicrobial Activity between Moroccan Lavender Species

Molecular Survey and Microscopic Examination of Haemoparasites Infecting Lizards from Morocco

Effect of Adding Thymus leptobotrys on Extra Virgin Olive Oil’s Quality and Physicochemical Parameters

...

are the top articles of Abdelaziz Abbad at Université Cadi Ayyad.

What are Abdelaziz Abbad's research interests?

The research interests of Abdelaziz Abbad are: Valorization and Conservation of Medicinal and Aromatic Plants

What is Abdelaziz Abbad's total number of citations?

Abdelaziz Abbad has 2,551 citations in total.

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