Abbasali Eskandarian

About Abbasali Eskandarian

Abbasali Eskandarian, With an exceptional h-index of 11 and a recent h-index of 10 (since 2020), a distinguished researcher at Isfahan University of Medical Sciences, specializes in the field of malaria, toxoplasmosis, medical protozology.

His recent articles reflect a diverse array of research interests and contributions to the field:

Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence

Inhibition of mouse colon cancer growth following immunotherapy with a fraction of hydatid cyst fluid

Evaluation of expression variations in virulence-related genes of Leishmania major after several culture passages compared with Phlebotomus papatasi isolated …

Misdiagnosis of Plasmodium vivax in a Case of Mixed Malaria, Lead to Wrong Anti-Cancer Chemotherapy, Splenectomy, and Partial Hepatectomy Due to Relapse: A Case Report

A Triplex Nested PCR for Diagnosis of Plasmodium, Babesia and Toxoplasma in Blood to Promote Safety of Blood Transfusion Samples

Evaluation of Th17 immune responses of recombinant DNA vaccine encoding GRA14 and ROP13 genes against Toxoplasma gondii in BALB/c mice

A Triplex Nested Polymerase Chain Reaction for Simultaneously Diagnosis of Plasmodium, Babesia, and Toxoplasma in Blood to Promote Safety of Blood Transfusion

Knocking down of the DHFR-TS gene in Toxoplasma gondii using siRNA and assessing the subsequences on toxoplasmosis in mice

Abbasali Eskandarian Information

University

Isfahan University of Medical Sciences

Position

Associate professor of medical parasitology

Citations(all)

334

Citations(since 2020)

194

Cited By

213

hIndex(all)

11

hIndex(since 2020)

10

i10Index(all)

12

i10Index(since 2020)

10

Email

University Profile Page

Isfahan University of Medical Sciences

Abbasali Eskandarian Skills & Research Interests

malaria

toxoplasmosis

medical protozology

Top articles of Abbasali Eskandarian

Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence

Authors

Tayebeh Zohrabi,Mojtaba Azimi-Resketi,Fereshteh Talaei,Maryam Yaghoubi,Mohamad Reza Ganjalikhany,Farzaneh Mohamadi Farsani,Abbasali Eskandarian

Journal

Experimental Parasitology

Published Date

2023/8/1

Toxoplasmosis is a serious parasitic infection and novel therapeutic options are highly demanded to effectively eliminate it. In current study, Toxoplasma gondii myosin A, C and F genes were knocked down using small interference RNA (siRNA) method and the parasite survival and virulence was evaluated in vitro and in vivo. The parasites were transfected with specific siRNA, virtually designed for myosin mRNAs, and co-cultured with human foreskin fibroblasts. The transfection rate and the viability of the transfected parasites were measured using flow cytometry and methyl thiazole tetrazolium (MTT) assays, respectively. Finally, the survival of BALB/c mice infected with siRNAs-transfected T. gondii was assessed. It was demonstrated that a transfection rate of 75.4% existed for siRNAs, resulting in 70% (P = 0.032), 80.6% (P = 0.017) and 85.5% (P = 0.013) gene suppression for myosin A, C and F in affected …

Inhibition of mouse colon cancer growth following immunotherapy with a fraction of hydatid cyst fluid

Authors

Shahla Rostamirad,Shima Daneshpour,Mohammad Reza Mofid,Alireza Andalib,Abbasali Eskandariyan,Somayeh Mousavi,Hossein Yousofi Darani

Journal

Experimental Parasitology

Published Date

2023/6/1

BackgroundHydatid cyst is the larval stage of the tape worm Echinococcus granulosus which is located in human and livestock viscera. There are some scientific evidences indicating that parasitic infections induce antitumor activity against certain types of cancers. In this study, the effects of a fraction of hydatid cyst fluid on colon cancer tumor in BALB/c mice were investigated.Materials and methodsIn this experimental work six groups of mice were challenged with mouse colon cancer cells. 5 days later when the sign of tumor growth in mice was seen, group 1–4 were injected with hydatid cyst fluid, the 78 kDa fraction, live protoscolices and BCG respectively. Group five was injected with alum alone and the sixth group left intact without any injection. The size of the tumor was measured and compared in all groups. Then blood samples of mice were evaluated for serum cytokine levels.ResultIn mice injected with …

Evaluation of expression variations in virulence-related genes of Leishmania major after several culture passages compared with Phlebotomus papatasi isolated …

Authors

Taher Nemati Haravani,Parviz Parvizi,Seyed Hossein Hejazi,Mohammad Mehdi Sedaghat,Abbasali Eskandarian,Mahmoud Nateghi Rostami

Journal

Plos one

Published Date

2023/4/13

Cutaneous leishmaniasis (CL) is a prevalent infectious disease with considerable morbidity annually. Here, we aimed to investigate the likely variations in gene expression of glycoprotein63 (gp63), heat shock protein 70 (HSP70), histone, arginase, cysteine protease B (CPB), Leishmania homologue of receptors for activated C kinase (LACK), small hydrophilic endoplasmic reticulum-associated protein (SHERP) in metacyclic promastigotes of L. major isolated from Phlebotomus papatasi sand flies and promastigotes excessively cultured in culture medium. The parasites were collected from suspected CL cases in Pasteur Institute of Iran, cultured and inoculated into the female BALB/c mice (2×106 promastigotes). Sand flies were trapped in Qom province, fed with the blood of euthanized infected mice and subsequently dissected in order to isolate the midgut including stomodeal valve. The metacyclic promastigotes were isolated from Ph. papatasi (Pro-Ppap) using peanut agglutinin test (PNA), then continuously cultured in RPMI-1640 medium enriched with fetal bovine serum, penicillin (100 U/ml) and streptomycin (100 mg/ml) to reach stationary phase (Pro-Stat). The gene expression was evaluated in both parasitic stages (Pro-Ppap and Pro-Stat) using qRT-PCR. Out results showed a significant increased gene expression at Pro-Ppap stage for gp63 (P = 0.002), SHERP (P = 0.001) and histone (P = 0.026) genes, in comparison with Pro-Stat stage. Noticeably, significant changes were, also, demonstrated in 10th to 15th passages [gp63 (P = 0.041), arginase (P = 0.016), LACK (P = 0.025)] and in 5th to 20th passage (SHERP) (P = 0.029). In …

Misdiagnosis of Plasmodium vivax in a Case of Mixed Malaria, Lead to Wrong Anti-Cancer Chemotherapy, Splenectomy, and Partial Hepatectomy Due to Relapse: A Case Report

Authors

Elahe Nasri,Abbasali Eskandarian

Journal

Iranian Journal of Parasitology

Published Date

2022/7

Malaria is a multilateral parasitic infection, which causes wonderful mortality and morbidity worldwide. It sometimes accompanied a quaint appearance. An Iranian 50-year-old man was admitted to Omid, hospital, a specialized cancer hospital in Isfahan, Iran. Because of a 15-year persisted anemia due to misdiagnose of vivax malaria led him to three courses of anticancer chemotherapy and splenectomy. His blood smears were sent to the Department of Parasitology, School of Medicine, Isfahan University of Medical Sciences, Iran. Our findings from his history, file documents, clinical signs and symptoms, and parasitological and molecular assessments revealed an interesting case, which is reported.

A Triplex Nested PCR for Diagnosis of Plasmodium, Babesia and Toxoplasma in Blood to Promote Safety of Blood Transfusion Samples

Authors

Abbasali Eskandarian

Journal

Iranian Journal of Parasitology

Published Date

2022/4

Background:The risk of transmission of some infectious agents has always been an important life-threatening side effect of blood transfusion. The aim of this study was to develop a triplex nested Polymerase Chain Reaction (tnPCR) to assess the presence of protozoan parasites Plasmodium, Toxoplasma and Babesia in blood samples concurrently. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of developed tnPCR method compared to gold standard methods were determined for all three parasite genera.Methods:After selecting the genus level specific primers and setting up of tnPCR, blood samples were collected partially from Isfahan Blood Transfusion Organization (IBTO). Some different samples from human and animal were tested by this method in comparison with the gold standard methods (microscopic method for Plasmodium, Babesia and ELISA …

Evaluation of Th17 immune responses of recombinant DNA vaccine encoding GRA14 and ROP13 genes against Toxoplasma gondii in BALB/c mice

Authors

Mohammad Fatollahzadeh,Abbasali Eskandarian,Hossein Yousofi Darani,Abdol Sattar Pagheh,Ehsan Ahmadpour

Journal

Infection, Genetics and Evolution

Published Date

2021/12/1

Toxoplasma gondii, a worldwide opportunistic parasite, causes serious diseases in both humans and fetuses with defective immune systems. The development of an effective vaccine is urgently required to prevent and control the spread of toxoplasmosis, caused by the apicomplexan parasite Toxoplasma gondii which is one of the most damaging zoonotic diseases of global importance. Plasmid DNA vaccination is a promising procedure for vaccine development and following the previous studies, pcROP13 + pcGRA14 cocktail DNA vaccine was evaluated for Th17 immune responses. Four groups of BALB/c mice were immunized intramuscularly three times at 2-week intervals. Subsequently, the production of anti- T. gondii antibodies and serum levels of cytokines IL-17, and IL-22 were evaluated against the RH strain of T. gondii. In addition, both the reactive oxygen species (ROS) and parasite load were assessed …

A Triplex Nested Polymerase Chain Reaction for Simultaneously Diagnosis of Plasmodium, Babesia, and Toxoplasma in Blood to Promote Safety of Blood Transfusion

Authors

Abbasali Eskandarian,Alireza Najafirad

Journal

Journal of Isfahan Medical School

Published Date

2021/9/23

Background Diagnosis and remove of blood transmittable infections has a vital importance in blood transfusion. The aim of this study was to concurrently assess the presence of protozoan parasites plasmodium, toxoplasma, and babesia in samples of blood transfusion.Methods In this experimental study, the development of a triplex nested-Polymerase chain reaction (PCR) for extol of blood transfusion process at 2020 in Isfahan City, Iran, was done. After selecting the genus specific primers, at the first step, nested PCRs were set up for each parasite specifically at the genus level using blood samples with a determined number of each parasite, and then multiplex nested PCR was set up.Findings The nested PCR triple reaction (TN-PCR) has a higher diagnostic validity compared to the microscopic and enzyme-linked immunosorbent assay (ELISA) methods. Sensitivity, specificity, and positive and negative predictive values were as 100%, 100%, 100%, and 100%, respectively, for detection Plasmodium, 100%, 91%, 92%, and 100%, for Toxoplasma, and 94%, 100%, 100%, and 89% for Babesia. The validity of this test for Plasmodium was the highest compared to the other two parasites.Conclusion Based on the results of this study, the TN-PCR method can simultaneously identify one, two or all three dangerous pathogens in biological samples as well as blood samples in the blood transfusion process.

Knocking down of the DHFR-TS gene in Toxoplasma gondii using siRNA and assessing the subsequences on toxoplasmosis in mice

Authors

Mojtaba Azimi-Resketi,Abbasali Eskandarian,Mazdak Ganjalikhani-Hakemi,Tayebeh Zohrabi

Journal

Acta tropica

Published Date

2020/7/1

Toxoplasma gondii (T. gondii), an obligatory intracellular parasite, is the etiologic agent of toxoplasmosis. Dihydrofolate reductase-thymidylate synthase (DHFR-TS) is one of the most important enzymes in toxoplasma folic acid cycle. Due to the emergence of resistance in RH strain of T. gondii against pyrimethamine that acts via DHFR-TS inhibition and also the crucial role of small interference RNA (siRNA) technology in gene silencing, we aimed to use siRNA to knock down DHFR-TS gene expression in T. gondii as a therapeutic target against toxoplasmosis in a mouse model.Based on the DHFR-TS gene sequence, siRNA was designed. The siRNAs were transfected into the parasites by electroporation. Total RNA was extracted using RNX-Plus kit. The viability of parasite was assessed by methylthiazole tetrazolium (MTT). The survival time of mice challenged with siRNA-treated T.gondii were compared to the …

See List of Professors in Abbasali Eskandarian University(Isfahan University of Medical Sciences)

Abbasali Eskandarian FAQs

What is Abbasali Eskandarian's h-index at Isfahan University of Medical Sciences?

The h-index of Abbasali Eskandarian has been 10 since 2020 and 11 in total.

What are Abbasali Eskandarian's top articles?

The articles with the titles of

Knocking down the expression of the molecular motors, myosin A, C and F genes in Toxoplasma gondii to decrease the parasite virulence

Inhibition of mouse colon cancer growth following immunotherapy with a fraction of hydatid cyst fluid

Evaluation of expression variations in virulence-related genes of Leishmania major after several culture passages compared with Phlebotomus papatasi isolated …

Misdiagnosis of Plasmodium vivax in a Case of Mixed Malaria, Lead to Wrong Anti-Cancer Chemotherapy, Splenectomy, and Partial Hepatectomy Due to Relapse: A Case Report

A Triplex Nested PCR for Diagnosis of Plasmodium, Babesia and Toxoplasma in Blood to Promote Safety of Blood Transfusion Samples

Evaluation of Th17 immune responses of recombinant DNA vaccine encoding GRA14 and ROP13 genes against Toxoplasma gondii in BALB/c mice

A Triplex Nested Polymerase Chain Reaction for Simultaneously Diagnosis of Plasmodium, Babesia, and Toxoplasma in Blood to Promote Safety of Blood Transfusion

Knocking down of the DHFR-TS gene in Toxoplasma gondii using siRNA and assessing the subsequences on toxoplasmosis in mice

are the top articles of Abbasali Eskandarian at Isfahan University of Medical Sciences.

What are Abbasali Eskandarian's research interests?

The research interests of Abbasali Eskandarian are: malaria, toxoplasmosis, medical protozology

What is Abbasali Eskandarian's total number of citations?

Abbasali Eskandarian has 334 citations in total.

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