Aaron Welch

Aaron Welch

Florida International University

H-index: 6

North America-United States

About Aaron Welch

Aaron Welch, With an exceptional h-index of 6 and a recent h-index of 5 (since 2020), a distinguished researcher at Florida International University,

His recent articles reflect a diverse array of research interests and contributions to the field:

A Yeast-Based Screening System for Differential Identification of Poisons and Suppressors of Human Topoisomerase I

Aaron Welch Information

University

Florida International University

Position

Assistant Professor of Biology

Citations(all)

187

Citations(since 2020)

119

Cited By

118

hIndex(all)

6

hIndex(since 2020)

5

i10Index(all)

5

i10Index(since 2020)

4

Email

University Profile Page

Florida International University

Top articles of Aaron Welch

A Yeast-Based Screening System for Differential Identification of Poisons and Suppressors of Human Topoisomerase I

Authors

Ahmed Seddek,Christian Madeira,Thirunavukkarasu Annamalai,Christopher Mederos,Purushottam B. Tiwari,Aaron Z. Welch,Yuk-Ching Tse-Dinh

Journal

Frontiers in Bioscience (Landmark Ed)

Published Date

2022

Background Inhibition of human topoisomerase I (TOP1) by camptothecin and topotecan has been shown to reduce excessive transcription of PAMP (Pathogen-Associated Molecular Pattern)-induced genes in prior studies, preventing death from sepsis in animal models of bacterial and SARS-CoV-2 infections. The TOP1 catalytic activity likely resolves the topological constraints on DNA that encodes these genes to facilitate the transcription induction that leads to excess inflammation. The increased accumulation of TOP1-DNA covalent complex (TOP1cc) following DNA cleavage is the basis for the anticancer efficacy of the TOP1 poisons developed for anticancer treatment. The potential cytotoxicity and mutagenicity of TOP1 targeting cancer drugs pose serious concerns for employing them as therapies in sepsis prevention. Methods In this study we set up a novel yeast-based screening system that employs yeast strains expressing wild-type or a dominant lethal mutant recombinant human TOP1. The effect of test compounds on growth is monitored with and without overexpression of the recombinant human TOP1. Results This yeast-based screening system can identify human TOP1 poisons for anticancer efficacy as well as TOP1 suppressors that can inhibit TOP1 DNA binding or cleavage activity in steps prior to the formation of the TOP1cc. Conclusions This yeast-based screening system can distinguish between TOP1 suppressors and TOP1 poisons. The assay can also identify compounds that are likely to be cytotoxic based on their effect on yeast cell growth that is independent of recombinant human TOP1 overexpression.

See List of Professors in Aaron Welch University(Florida International University)

Aaron Welch FAQs

What is Aaron Welch's h-index at Florida International University?

The h-index of Aaron Welch has been 5 since 2020 and 6 in total.

What are Aaron Welch's top articles?

The articles with the titles of

A Yeast-Based Screening System for Differential Identification of Poisons and Suppressors of Human Topoisomerase I

are the top articles of Aaron Welch at Florida International University.

What is Aaron Welch's total number of citations?

Aaron Welch has 187 citations in total.

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