Aaron Stevens

Aaron Stevens

University of Otago

H-index: 11

Oceania-New Zealand

About Aaron Stevens

Aaron Stevens, With an exceptional h-index of 11 and a recent h-index of 10 (since 2020), a distinguished researcher at University of Otago, specializes in the field of epigenetics, inflammation and DNA structure.

His recent articles reflect a diverse array of research interests and contributions to the field:

Chromosomal instability and its effect on cell lines

Site-specific decreases in DNA methylation in replicating cells following exposure to oxidative stress

G-quadruplex ligands as therapeutic agents against cancer, neurological disorders and viral infections

The dynamic regulation of G-quadruplex DNA structures by cytosine methylation

Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells

The driver of extreme human-specific Olduvai repeat expansion remains highly active in the human genome

Author Correction: Human gut microbiome changes during a 10 week Randomised Control Trial for micronutrient supplementation in children with attention deficit hyperactivity …

Aaron Stevens Information

University

University of Otago

Position

NZ

Citations(all)

376

Citations(since 2020)

313

Cited By

183

hIndex(all)

11

hIndex(since 2020)

10

i10Index(all)

11

i10Index(since 2020)

10

Email

University Profile Page

University of Otago

Aaron Stevens Skills & Research Interests

epigenetics

inflammation and DNA structure

Top articles of Aaron Stevens

Chromosomal instability and its effect on cell lines

Authors

Zichen He,Andrew Wilson,Fenella Rich,Diane Kenwright,Aaron Stevens,Yee Syuen Low,Michelle Thunders

Published Date

2023/6

Background Cancer cell lines are invaluable model systems for biomedical research because they provide an almost unlimited supply of biological materials. However, there is considerable skepticism regarding the reproducibility of data derived from these in vitro models. Recent findings Chromosomal instability (CIN) is one of the primary issues associated with cell lines, which can cause genetic heterogeneity and unstable cell properties within a cell population. Many of these problems can be avoided with some precautions. Here we review the underlying causes of CIN, including merotelic attachment, telomere dysfunction, DNA damage response defects, mitotic checkpoint defects and cell cycle disturbances. Conclusion In this review we summarize studies highlighting the consequences of CIN in various cell lines and provide suggestions on monitoring and controlling CIN during cell culture.

Site-specific decreases in DNA methylation in replicating cells following exposure to oxidative stress

Authors

Annika R Seddon,Andrew B Das,Mark B Hampton,Aaron J Stevens

Journal

Human Molecular Genetics

Published Date

2023/2/15

Oxidative stress is a common feature of inflammation-driven cancers, and it promotes genomic instability and aggressive tumour phenotypes. It is known that oxidative stress transiently modulates gene expression through the oxidation of transcription factors and associated regulatory proteins. Neutrophils are our most abundant white blood cells and accumulate at sites of infection and inflammation. Activated neutrophils produce hypochlorous acid and chloramines, which can disrupt DNA methylation by oxidizing methionine. The goal of the current study was to determine whether chloramine exposure results in sequence-specific modifications in DNA methylation that enable long-term alterations in transcriptional output. Proliferating Jurkat T-lymphoma cells were exposed to sublethal doses of glycine chloramine and differential methylation patterns were compared using Illumina EPIC 850 K bead chip arrays …

G-quadruplex ligands as therapeutic agents against cancer, neurological disorders and viral infections

Authors

Mock Phooi Yan,Chua Eng Wee,Khor Poh Yen,Aaron Stevens,Lam Kok Wai

Published Date

2023/11

G-quadruplexes (G4s) within the human genome have undergone extensive molecular investigation, with a strong focus on telomeres, gene promoters and repetitive regulatory sequences. G4s play central roles in regulating essential biological processes, including telomere maintenance, replication, transcription and translation. Targeting these molecular processes with G4-binding ligands holds substantial therapeutic potential in anticancer treatments and has also shown promise in treating neurological, skeletal and muscular disorders. The presence of G4s in bacterial and viral genomes also suggests that G4-binding ligands could be a critical tool in fighting infections. This review provides an overview of the progress and applications of G4-binding ligands, their proposed mechanisms of action, challenges faced and prospects for their utilization in anticancer treatments, neurological disorders and antiviral …

The dynamic regulation of G-quadruplex DNA structures by cytosine methylation

Authors

Aaron John Stevens,Lucy de Jong,Martin Alexander Kennedy

Published Date

2022/2/22

It is well known that certain non B-DNA structures, including G-quadruplexes, are key elements that can regulate gene expression. Here, we explore the theory that DNA modifications, such as methylation of cytosine, could act as a dynamic switch by promoting or alleviating the structural formation of G-quadruplex structures in DNA or RNA. The interaction between epigenetic DNA modifications, G4 formation, and the 3D architecture of the genome is a complex and developing area of research. Although there is growing evidence for such interactions, a great deal still remains to be discovered. In vivo, the potential effect that cytosine methylation may have on the formation of DNA structures has remained largely unresearched, despite this being a potential mechanism through which epigenetic factors could regulate gene activity. Such interactions could represent novel mechanisms for important biological functions, including altering nucleosome positioning or regulation of gene expression. Furthermore, promotion of strand-specific G-quadruplex formation in differentially methylated genes could have a dynamic role in directing X-inactivation or the control of imprinting, and would be a worthwhile focus for future research.

Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells

Authors

Annika R Seddon,Yusmiati Liau,Paul E Pace,Allison L Miller,Andrew B Das,Martin A Kennedy,Mark B Hampton,Aaron J Stevens

Journal

Epigenetics & Chromatin

Published Date

2021/12

Background Environmental factors, such as oxidative stress, have the potential to modify the epigenetic landscape of cells. We have previously shown that DNA methyltransferase (DNMT) activity can be inhibited by sublethal doses of hydrogen peroxide (H2O2). However, site-specific changes in DNA methylation and the reversibility of any changes have not been explored. Using bead chip array technology, differential methylation was assessed in Jurkat T-lymphoma cells following exposure to H2O2. Results Sublethal H2O2 exposure was associated with an initial genome-wide decrease in DNA methylation in replicating cells, which was largely corrected 72 h later. However, some alterations were conserved through subsequent cycles of cell division. Significant changes to the variability of DNA methylation were also observed both globally and at the …

The driver of extreme human-specific Olduvai repeat expansion remains highly active in the human genome

Authors

Ilea E Heft,Yulia Mostovoy,Michal Levy-Sakin,Walfred Ma,Aaron J Stevens,Steven Pastor,Jennifer McCaffrey,Dario Boffelli,David I Martin,Ming Xiao,Martin A Kennedy,Pui-Yan Kwok,James M Sikela

Journal

Genetics

Published Date

2020/1/1

Olduvai (formerly DUF1220) protein domains have undergone the largest human-specific increase in copy number of any coding region in the genome (∼300 copies of which 165 are human-specific) and have been implicated in human brain evolution... Sequences encoding Olduvai protein domains (formerly DUF1220) show the greatest human lineage-specific increase in copy number of any coding region in the genome and have been associated, in a dosage-dependent manner, with brain size, cognitive aptitude, autism, and schizophrenia. Tandem intragenic duplications of a three-domain block, termed the Olduvai triplet, in four NBPF genes in the chromosomal 1q21.1-0.2 region, are primarily responsible for the striking human-specific copy number increase. Interestingly, most of the Olduvai triplets are adjacent to, and transcriptionally coregulated with, three human-specific NOTCH2NL genes that …

Author Correction: Human gut microbiome changes during a 10 week Randomised Control Trial for micronutrient supplementation in children with attention deficit hyperactivity …

Authors

Aaron J Stevens,Rachel V Purcell,Kathryn A Darling,Matthew JF Eggleston,Martin A Kennedy,Julia J Rucklidge

Journal

Scientific Reports

Published Date

2020

“Pairwise difference plots for Alpha diversity metrics. Treatment group is represented on the x-axis and pairwise change between pre and post measurements are demonstrated on the y-axis. The asterisk denotes significance (p= 0.05).(A) Pairwise difference comparison in observed OTUs (Alpha diversity).(B) Pairwise difference comparison of community richness using Shannon indices (Beta diversity).” should read:

See List of Professors in Aaron Stevens University(University of Otago)

Aaron Stevens FAQs

What is Aaron Stevens's h-index at University of Otago?

The h-index of Aaron Stevens has been 10 since 2020 and 11 in total.

What are Aaron Stevens's top articles?

The articles with the titles of

Chromosomal instability and its effect on cell lines

Site-specific decreases in DNA methylation in replicating cells following exposure to oxidative stress

G-quadruplex ligands as therapeutic agents against cancer, neurological disorders and viral infections

The dynamic regulation of G-quadruplex DNA structures by cytosine methylation

Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells

The driver of extreme human-specific Olduvai repeat expansion remains highly active in the human genome

Author Correction: Human gut microbiome changes during a 10 week Randomised Control Trial for micronutrient supplementation in children with attention deficit hyperactivity …

are the top articles of Aaron Stevens at University of Otago.

What are Aaron Stevens's research interests?

The research interests of Aaron Stevens are: epigenetics, inflammation and DNA structure

What is Aaron Stevens's total number of citations?

Aaron Stevens has 376 citations in total.

    academic-engine

    Useful Links