Abdelahhad Barbour

Abdelahhad Barbour

University of Toronto

H-index: 9

North America-Canada

Professor Information

University

University of Toronto

Position

___

Citations(all)

358

Citations(since 2020)

270

Cited By

135

hIndex(all)

9

hIndex(since 2020)

8

i10Index(all)

8

i10Index(since 2020)

7

Email

University Profile Page

University of Toronto

Research & Interests List

Molecular Microbiology

Top articles of Abdelahhad Barbour

Practical 10‐Color T‐Cell Panel for Phenotyping Diverse Populations Using Spectral Flow Cytometry: A Beginner's Guide

Flow cytometry stands as the most employed high‐throughput single‐cell analysis technique, facilitating the profiling of remarkably diverse samples, such as blood, bone marrow and body fluids. In addition, it allows for the discrimination of diverse immune cell subsets, including infrequently encountered types like T regulatory cells and exhausted CD28Null T cells. However, analyzing rare immune cell subsets with conventional flow cytometry poses challenges stemming from factors like fluorophore overlap, compensation issues, and limited flexibility in fluorophore selection. Therefore, spectral flow cytometry offers advantages over traditional flow cytometry. It measures the full emission spectrum and then separates it to identify different fluorochromes. This enables the use of fluorochromes with significant overlap in a single test, allowing for the analysis of more protein markers. Following this, spectral technology …

Authors

Sina Zargaran,Morvarid Oveisi,Chunxiang Sun,Abdelahhad Barbour,Michael Glogauer

Journal

Current Protocols

Published Date

2024/3

Effects of a stabilized stannous fluoride dentifrice on clinical, immunomodulatory, and microbial outcomes in a human experimental gingivitis model

Background Stannous fluoride dentifrice is well established for its beneficial clinical effects. In this study, we evaluated the effects of stannous fluoride on inflammation and oral microbiome. Methods In this randomized, parallel‐arm, double‐blind, controlled clinical trial, we compared clinical resolution of experimental gingivitis by evaluating bleeding on probing, gingival index, and plaque index between stannous fluoride stabilized with zinc phosphate (test) and sodium fluoride (control) dentifrices. Further, these groups were compared for oral neutrophil counts, systemic priming of neutrophils, gingival crevicular fluid (GCF) expression of inflammatory markers, and the oral microbiome. Results We found significant reduction in bleeding on probing in the test group compared to the control group in experimental gingivitis when participants used the test dentifrice prior to induction of experimental gingivitis. The test …

Authors

Noah Fine,Abdelahhad Barbour,Kamini Kaura,Kristopher A Kerns,Dandan Chen,Harsh M Trivedi,Juliana Gomez,Amarpreet Sabharwal,Jeffrey S McLean,Richard P Darveau,Michael Glogauer

Journal

Journal of Periodontology

Published Date

2023/10/27

Discovery of phosphorylated lantibiotics with proimmune activity that regulate the oral microbiome

Lantibiotics are ribosomally synthesized and posttranslationally modified peptides (RiPPs) that are produced by bacteria. Interest in this group of natural products is increasing rapidly as alternatives to conventional antibiotics. Some human microbiome–derived commensals produce lantibiotics to impair pathogens’ colonization and promote healthy microbiomes. Streptococcus salivarius is one of the first commensal microbes to colonize the human oral cavity and gastrointestinal tract, and its biosynthesis of RiPPs, called salivaricins, has been shown to inhibit the growth of oral pathogens. Herein, we report on a phosphorylated class of three related RiPPs, collectively referred to as salivaricin 10, that exhibit proimmune activity and targeted antimicrobial properties against known oral pathogens and multispecies biofilms. Strikingly, the immunomodulatory activities observed include upregulation of neutrophil-mediated …

Authors

Abdelahhad Barbour,Leif Smith,Morvarid Oveisi,McKinley Williams,Ruo Chen Huang,Cara Marks,Noah Fine,Chunxiang Sun,Fereshteh Younesi,Sina Zargaran,Ravi Orugunty,Thomas D Horvath,Sigmund J Haidacher,Anthony M Haag,Amarpreet Sabharwal,Boris Hinz,Michael Glogauer

Journal

Proceedings of the National Academy of Sciences

Published Date

2023/5/30

Metabolites of the oral microbiome: Important mediators of multikingdom interactions

The oral cavity hosts over 700 different microbial species that produce a rich reservoir of bioactive metabolites critical to oral health maintenance. Over the last two decades, new insights into the oral microbiome and its importance in health and disease have emerged mainly due to the discovery of new oral microbial species using next-generation sequencing. This advancement has revolutionized the documentation of unique microbial profiles associated with different niches and health/disease states within the oral cavity and the relation of the oral bacteria to systemic diseases. However, less work has been done to identify and characterize the unique oral microbial metabolites that play critical roles in maintaining equilibrium between the various oral microbial species and their human hosts. This article discusses the most significant microbial metabolites produced by these diverse communities of oral bacteria …

Authors

Abdelahhad Barbour,Omnia Elebyary,Noah Fine,Morvarid Oveisi,Michael Glogauer

Published Date

2022/1

The effect of intensity-modulated radiotherapy to the head and neck region on the oral innate immune response and oral microbiome: A prospective cohort study of head and neck …

Neutrophils, also known as polymorphonuclear leukocytes (PMNs), form a significant component of the innate host response, and the consequence of the interaction between the oral microbiota and PMNs is a crucial determinant of oral health status. The impact of radiation therapy (RT) for head and neck tumour (HNT) treatment on the oral innate immune system, neutrophils in particular, and the oral microbiome has not been thoroughly investigated. Therefore, the objective of this study was to characterize RT-mediated changes in oral neutrophils (oPMNs) and the oral microbiome in patients undergoing RT to treat HNTs. Oral rinse samples were collected prior to, during and post-RT from HNT patients receiving RT at Dental Oncology at Princess Margaret Cancer Centre. The oPMNs counts and activation states were analysed using flow cytometry, and the oral microbiome was analysed using 16S rRNA gene sequencing. Statistically significant (p < 0.05) drops in oPMN counts and the activation states of the CD11b, CD16, CD18, CD64 and H3Cit markers from pre-RT to post-RT were observed. Moreover, exposure to RT caused a significant reduction in the relative abundance of commensal Gram-negative bacteria and increased the commensal Gram-positive microbes. Ionizing radiation for the treatment of HNTs simultaneously decreased the recruitment of oPMNs into the oral cavity and suppressed their activation state. The oral microbiome composition post-RT was altered significantly due to RT which may favour the colonization of specific microbial communities unfavourable for the long-term development of a balanced oral microbiome.

Authors

Zahra Dorna Mojdami,Abdelahhad Barbour,Morvarid Oveisi,Chunxiang Sun,Noah Fine,Sourav Saha,Cara Marks,Omnia Elebyary,Erin Watson,Howard Tenenbaum,Amir Azarpazhooh,Michael Glogauer

Journal

International Journal of Molecular Sciences

Published Date

2022/8/24

Periodontal inflammation primes the systemic innate immune response

The presence of periodontal diseases (PDs) often strongly correlates with other severe chronic inflammatory conditions, including cardiovascular disease, diabetes, and arthritis. However, the mechanisms through which these diseases interact are unclear. In PD, tissue and bone destruction in the mouth is driven by elevated recruitment of polymorphonuclear neutrophils (PMNs), which are primed and recruited from the circulation to sites of inflammation. We predicted that systemic effects on PMN mobilization or priming could account for the interaction between PD and other inflammatory conditions. We tested this using a mouse model of ligature-induced PD and found elevated PMN counts specifically in bone marrow, supporting a systemic effect of periodontal tissue inflammation on PMN production. In contrast, mice with induced peritonitis had elevated PMN counts in the blood, peritoneum, and colon. These …

Authors

N Fine,JW Chadwick,C Sun,KK Parbhakar,N Khoury,A Barbour,M Goldberg,HC Tenenbaum,M Glogauer

Journal

Journal of dental research

Published Date

2021/3

The crossroads of periodontitis and oral squamous cell carcinoma: immune implications and tumor promoting capacities

Periodontitis (PD) is increasingly considered to interact with and promote a number of inflammatory diseases, including cancer. In the case of oral squamous cell carcinoma (OSCC) the local inflammatory response associated with PD is capable of triggering altered cellular events that can promote cancer cell invasion and proliferation of existing primary oral carcinomas as well as supporting the seeding of metastatic tumor cells into the gingival tissue giving rise to secondary tumors. Both the immune and stromal components of the periodontium exhibit phenotypic alterations and functional differences during PD that result in a microenvironment that favors cancer progression. The inflammatory milieu in PD is ideal for cancer cell seeding, migration, proliferation and immune escape. Understanding the interactions governing this attenuated anti-tumor immune response is vital to unveil unexplored preventive or therapeutic possibilities. Here we review the many commonalities between the oral-inflammatory microenvironment in PD and oral-inflammatory responses that are associated with OSCC progression, and how these conditions can act to promote and sustain the hallmarks of cancer.

Authors

Omnia Elebyary,Abdelahhad Barbour,Noah Fine,Howard C Tenenbaum,Michael Glogauer

Published Date

2021/1/20

Evolution of Lantibiotic Salivaricins: New Weapons to Fight Infectious Diseases

Lantibiotic salivaricins are polycyclic peptides containing lanthionine and/or β-methyllanthionine residues produced by certain strains of Streptococcus salivarius, which almost exclusively reside in the human oral cavity. The importance of these molecules stems from their antimicrobial activity towards relevant oral pathogens which has so far been applied through the development of salivaricin-producing probiotic strains. However, salivaricins may also prove to be of great value in the development of new and novel antibacterial therapies in this era of emerging antibiotic resistance. In this review, we describe the biosynthesis, antimicrobial activity, structure, and mode of action of the lantibiotic salivaricins characterized to date. Moreover, we also provide an expert opinion and suggestions for future development of this important field of microbiology.

Authors

Abdelahhad Barbour,Philip Wescombe,Leif Smith

Journal

Trends In Microbiology

Published Date

2020

Professor FAQs

What is Abdelahhad Barbour's h-index at University of Toronto?

The h-index of Abdelahhad Barbour has been 8 since 2020 and 9 in total.

What are Abdelahhad Barbour's research interests?

The research interests of Abdelahhad Barbour are: Molecular Microbiology

What is Abdelahhad Barbour's total number of citations?

Abdelahhad Barbour has 358 citations in total.

What are the co-authors of Abdelahhad Barbour?

The co-authors of Abdelahhad Barbour are Michael Glogauer, Howard C Tenenbaum, Celine M Levesque, Osama K. Abou-Zied.

Co-Authors

H-index: 69
Michael Glogauer

Michael Glogauer

University of Toronto

H-index: 58
Howard C Tenenbaum

Howard C Tenenbaum

University of Toronto

H-index: 32
Celine M Levesque

Celine M Levesque

University of Toronto

H-index: 28
Osama K. Abou-Zied

Osama K. Abou-Zied

Sultan Qaboos University

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